The structure of type III CRISPR-associated deaminase in complex cA4
Li Y, Li Z, Yan P, Hua C, Kong J, Wu W, Cui Y, Duan Y, Li S, Li G, Ji S, Chen Y, Zhao Y, Yang P, Hu C, Lu M, Chen M, Xiao Y
Science (2025) 387 pp. eadr0393-eadr0393 [ DOI: doi:10.1126/science.adr0393 Pubmed: 39666823 ]
- Adenosine deaminase domain-containing protein (70 kDa, Protein from Limisphaera ngatamarikiensis)
- Zinc ion (65 Da, Ligand)
- Crispr-associated adenosine deaminase (Complex from Limisphaera ngatamarikiensis)
- 3'-o-[(r)-{[(2s,3as,4s,6s,6as)-6-(6-amino-9h-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2h-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine (658 Da, Ligand)
Shan Y, Zhao L, Chen M, Li X, Zhang M, Pei D
Nat Commun (2024) 15 pp. 7292- [ Pubmed: 39181878 ]
- Archaeal-type opsin 2 (34 kDa, Protein from Chlamydomonas reinhardtii)
- Endochr2 (Complex from Chlamydomonas reinhardtii)
- Retinal (284 Da, Ligand)
Cryo-EM structure of cryptophyte photosystem II
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8xr6
Q-score: 0.574
Zhang YZ, Li K, Qin BY, Guo JP, Zhang QB, Zhao DL, Chen XL, Gao J, Liu LN, Zhao LS
Nat Commun (2024) 15 pp. 4999-4999 [ Pubmed: 38866834 DOI: doi:10.1038/s41467-024-49453-0 ]
- Chlorophyll c2 (608 Da, Ligand)
- Photosystem ii reaction center protein x (4 kDa, Protein from Chroomonas placoidea)
- Psbq (15 kDa, Protein from Chroomonas placoidea)
- Photosystem ii reaction center protein l (4 kDa, Protein from Chroomonas placoidea)
- Photosystem ii d2 protein (39 kDa, Protein from Chroomonas placoidea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem ii reaction center protein k (5 kDa, Protein from Chroomonas placoidea)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psbw (13 kDa, Protein from Chroomonas placoidea)
- Photosystem ii reaction center protein i (4 kDa, Protein from Chroomonas placoidea)
- (6'r,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene (536 Da, Ligand)
- (1~{r})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e})-3,7,12,16-tetramethyl-18-[(4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol (564 Da, Ligand)
- Ca-mn4-o5 cluster (339 Da, Ligand)
- Acpii-2 (22 kDa, Protein from Chroomonas placoidea)
- Acpii-6 (25 kDa, Protein from Chroomonas placoidea)
- Photosystem ii reaction center protein j (3 kDa, Protein from Chroomonas placoidea)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Bicarbonate ion (61 Da, Ligand)
- Photosystem ii reaction center protein psb30 (3 kDa, Protein from Chroomonas placoidea)
- Photosystem ii reaction center protein t (3 kDa, Protein from Chroomonas placoidea)
- Pheophytin a (871 Da, Ligand)
- Photosystem ii protein d1 (39 kDa, Protein from Chroomonas placoidea)
- Fe (ii) ion (55 Da, Ligand)
- Psbm (3 kDa, Protein from Chroomonas placoidea)
- Psb-gama_linker (31 kDa, Protein from Chroomonas placoidea)
- (1~{r})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol (550 Da, Ligand)
- Psbc_cp43 (52 kDa, Protein from Chroomonas placoidea)
- Cytochrome b559 subunit alpha (9 kDa, Protein from Chroomonas placoidea)
- Acpii-3 (24 kDa, Protein from Chroomonas placoidea)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Photosystem ii reaction center protein z (6 kDa, Protein from Chroomonas placoidea)
- Photosystem ii of cryptophyte (1 MDa, Complex from Chroomonas placoidea)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Psbo (32 kDa, Protein from Chroomonas placoidea)
- Psbu (13 kDa, Protein from Chroomonas placoidea)
- Photosystem ii cytochrome c550 (17 kDa, Protein from Chroomonas placoidea)
- Water (18 Da, Ligand)
- Photosystem ii reaction center protein h (7 kDa, Protein from Chroomonas placoidea)
- Cytochrome b559 subunit beta (4 kDa, Protein from Chroomonas placoidea)
- Chloride ion (35 Da, Ligand)
- Acpii-5 (23 kDa, Protein from Chroomonas placoidea)
- Photosystem ii cp47 reaction center protein (56 kDa, Protein from Chroomonas placoidea)
- Acpii-4 (23 kDa, Protein from Chroomonas placoidea)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Acpii-1 (23 kDa, Protein from Chroomonas placoidea)
Cryo-EM structure of Symbiodinium photosystem I
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8jjr
Q-score: 0.575
Zhao LS, Wang N, Li K, Li CY, Guo JP, He FY, Liu GM, Chen XL, Gao J, Liu LN, Zhang YZ
Nat Commun (2024) 15 pp. 2392-2392 [ Pubmed: 38493166 DOI: doi:10.1038/s41467-024-46791-x ]
- Chlorophyll c2 (608 Da, Ligand)
- Psae (13 kDa, Protein from Symbiodinium sp.)
- Peridinin (630 Da, Ligand)
- Psad (32 kDa, Protein from Symbiodinium sp.)
- Beta-carotene (536 Da, Ligand)
- Pcpi-7 (27 kDa, Protein from Symbiodinium sp.)
- (3s,3'r,5r,6s,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol (582 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Pcpi-1 (28 kDa, Protein from Symbiodinium sp.)
- Psac (16 kDa, Protein from Symbiodinium sp.)
- Pcpi-3 (27 kDa, Protein from Symbiodinium sp.)
- Psaj (15 kDa, Protein from Symbiodinium sp.)
- Psam (14 kDa, Protein from Symbiodinium sp.)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Psal (38 kDa, Protein from Symbiodinium sp.)
- Pcpi-13 (23 kDa, Protein from Symbiodinium sp.)
- Pcpi-8 (30 kDa, Protein from Symbiodinium sp.)
- Pcp-11 (21 kDa, Protein from Symbiodinium sp.)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Photosystem i of symbiodinium (Complex from Symbiodinium sp.)
- Psat (13 kDa, Protein from Symbiodinium sp.)
- Psaf (29 kDa, Protein from Symbiodinium sp.)
- Psab (74 kDa, Protein from Symbiodinium sp.)
- Pcpi-4 (24 kDa, Protein from Symbiodinium sp.)
- Phylloquinone (450 Da, Ligand)
- Pcpi-5 (26 kDa, Protein from Symbiodinium sp.)
- Psaa (76 kDa, Protein from Symbiodinium sp.)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Psar (17 kDa, Protein from Symbiodinium sp.)
- Pcpi-2 (15 kDa, Protein from Symbiodinium sp.)
- Pcpi-9 (22 kDa, Protein from Symbiodinium sp.)
- Psai (19 kDa, Protein from Symbiodinium sp.)
- Psau (24 kDa, Protein from Symbiodinium sp.)
- [(1~{s},5~{r})-3,3,5-trimethyl-5-oxidanyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{s},4~{s},6~{r})-2,2,6-trimethyl-4-oxidanyl-7-oxabicyclo[4.1.0]heptan-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenylidene]cyclohexyl] ethanoate (642 Da, Ligand)
- Pcpi-12 (21 kDa, Protein from Symbiodinium sp.)
- Pcpi-10 (27 kDa, Protein from Symbiodinium sp.)
- Iron/sulfur cluster (351 Da, Ligand)
- Pcpi-6 (19 kDa, Protein from Symbiodinium sp.)
Rat Kir4.1 in complex with PIP2
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8i5m
Q-score: 0.486
Zhou X, Zhao C, Xu H, Xu Y, Zhan L, Wang P, He J, Lu T, Gu Y, Yang Y, Xu C, Chen Y, Liu Y, Zeng Y, Tian F, Chen Q, Xie X, Liu J, Hu H, Li J, Zheng Y, Guo J, Gao Z
Nat Chem Biol (2024) 20 pp. 857-866 [ DOI: doi:10.1038/s41589-024-01555-y Pubmed: 38355723 ]
- Rat kir4.1 in complex with pip2 (Complex from Rattus norvegicus)
- Atp-sensitive inward rectifier potassium channel 10 (38 kDa, Protein from Rattus norvegicus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Rat Kir4.1 in complex with PIP2 and Lys05
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8i5n
Q-score: 0.414
Zhou X, Zhao C, Xu H, Xu Y, Zhan L, Wang P, He J, Lu T, Gu Y, Yang Y, Xu C, Chen Y, Liu Y, Zeng Y, Tian F, Chen Q, Xie X, Liu J, Hu H, Li J, Zheng Y, Guo J, Gao Z
Nat Chem Biol (2024) 20 pp. 857-866 [ DOI: doi:10.1038/s41589-024-01555-y Pubmed: 38355723 ]
- Atp-sensitive inward rectifier potassium channel 10 (38 kDa, Protein from Rattus norvegicus)
- Rat kir4.1 in complex with pip2 and lys05 (Complex from Rattus norvegicus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8jru
Q-score: 0.438
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to beta-arrestin 1 (Complex from Homo sapiens)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Bos taurus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jrv
Q-score: 0.431
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023) 620 pp. 904-910 [ Pubmed: 37558880 DOI: doi:10.1038/s41586-023-06420-x ]
- The glucagon receptor bound to glucagon and beta-arrestin 1 (Complex from Homo sapiens)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Nanobody 32 (13 kDa, Protein from Escherichia phage EcSzw-2)
- Beta-arrestin 1 and single-chain fragment variable 30 (scfv30) (69 kDa, Protein from Homo sapiens)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Ha signal peptide,hpc4 purification tag,glucagon receptor,c-terminal tail of vasopressin v2 receptor (54 kDa, Protein from Homo sapiens)
Cryo-EM structure of cryptophyte photosystem I
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 7y7b
Q-score: 0.607
Zhao LS, Wang P, Li K, Zhang QB, He FY, Li CY, Su HN, Chen XL, Liu LN, Zhang YZ
Plant Cell (2023) 35 pp. 2449-2463 [ DOI: doi:10.1093/plcell/koad087 Pubmed: 36943796 ]
- Acpi-14 (23 kDa, Protein from Chroomonas placoidea)
- Chlorophyll c2 (608 Da, Ligand)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Chroomonas placoidea)
- Acpi-3 (24 kDa, Protein from Chroomonas placoidea)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Acpi-6 (22 kDa, Protein from Chroomonas placoidea)
- Acpi-7 (24 kDa, Protein from Chroomonas placoidea)
- Psao (10 kDa, Protein from Chroomonas placoidea)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chroomonas placoidea)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psar (13 kDa, Protein from Chroomonas placoidea)
- Acpi-9 (27 kDa, Protein from Chroomonas placoidea)
- (6'r,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene (536 Da, Ligand)
- Acpi-5 (24 kDa, Protein from Chroomonas placoidea)
- (1~{r})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e})-3,7,12,16-tetramethyl-18-[(4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-3,5,7,9,11,13,15-heptaen-1,17-diynyl]cyclohex-3-en-1-ol (564 Da, Ligand)
- (1~{r})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol (566 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Chroomonas placoidea)
- Photosystem i reaction center subunit iii (20 kDa, Protein from Chroomonas placoidea)
- Acpi-s (25 kDa, Protein from Chroomonas placoidea)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chroomonas placoidea)
- Photosystem i reaction center subunit psak (8 kDa, Protein from Chroomonas placoidea)
- Acpi-11 (22 kDa, Protein from Chroomonas placoidea)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Acpi-12 (23 kDa, Protein from Chroomonas placoidea)
- Acpi-13/10 (22 kDa, Protein from Chroomonas placoidea)
- (1~{r})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohexen-1-yl)octadeca-3,5,7,9,11,13,15,17-octaen-1-ynyl]cyclohex-3-en-1-ol (550 Da, Ligand)
- Photosystem i of cryptophyte (890 kDa, Complex from Chroomonas placoidea)
- Acpi-4 (23 kDa, Protein from Chroomonas placoidea)
- Phylloquinone (450 Da, Ligand)
- Unk1 (13 kDa, Protein from Chroomonas placoidea)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chroomonas placoidea)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chroomonas placoidea)
- Acpi-8 (23 kDa, Protein from Chroomonas placoidea)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Chroomonas placoidea)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Chroomonas placoidea)
- Acpi-1 (23 kDa, Protein from Chroomonas placoidea)
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chroomonas placoidea)
- Acpi-2 (23 kDa, Protein from Chroomonas placoidea)