Cryo-EM structure of the PSI-LHCI-Lhcp supercomplex from Ostreococcus tauri
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 7yca
Q-score: 0.514
Ishii A, Shan J, Sheng X, Kim E, Watanabe A, Yokono M, Noda C, Song C, Murata K, Liu Z, Minagawa J
eLife (2023) 12 [ Pubmed: 36951548 DOI: doi:10.7554/eLife.84488 ]
- Psag (14 kDa, Protein from Ostreococcus tauri)
- Phylloquinone (450 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (26 kDa, Protein from Ostreococcus tauri)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Ostreococcus tauri)
- Chlorophyll a-b binding protein, chloroplastic (29 kDa, Protein from Ostreococcus tauri)
- Beta-carotene (536 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Ostreococcus tauri)
- Photosystem i psan, reaction centre subunit n (14 kDa, Protein from Ostreococcus tauri)
- Photosystem i psah, reaction centre subunit vi (17 kDa, Protein from Ostreococcus tauri)
- Psi subunit v (21 kDa, Protein from Ostreococcus tauri)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Lhca6 (26 kDa, Protein from Ostreococcus tauri)
- Photosystem i reaction center subunit ii, chloroplastic (20 kDa, Protein from Ostreococcus tauri)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Ostreococcus tauri)
- Chlorophyll b (907 Da, Ligand)
- Chlorophyll c2 (608 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Ostreococcus tauri)
- Water (18 Da, Ligand)
- Photosystem i-light-harvesting complex i-prasinophyte-specific lhc protein (psi-lhci-lhcp) supercomplex (Complex from Ostreococcus tauri)
- Psao (15 kDa, Protein from Ostreococcus tauri)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Photosystem i reaction center subunit iii (24 kDa, Protein from Ostreococcus tauri)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction centre subunit iv (11 kDa, Protein from Ostreococcus tauri)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Ostreococcus tauri)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Ostreococcus tauri)
- (1~{s})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaenyl]cyclohex-3-en-1-ol (570 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Ostreococcus tauri)
- (3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-1-[(1~{s},4~{s})-2,2-dimethyl-6-methylidene-1,4-bis(oxidanyl)cyclohexyl]-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-2-one (600 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- Lhca1 (24 kDa, Protein from Ostreococcus tauri)
- Photosystem i iron-sulfur center (8 kDa, Protein from Ostreococcus tauri)
- Photosystem i psag/psak protein (13 kDa, Protein from Ostreococcus tauri)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Cryo-EM structure of the Lhcp complex from Ostreococcus tauri
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8hg3
Q-score: 0.479
Ishii A, Shan J, Sheng X, Kim E, Watanabe A, Yokono M, Noda C, Song C, Murata K, Liu Z, Minagawa J
eLife (2023) 12 [ Pubmed: 36951548 DOI: doi:10.7554/eLife.84488 ]
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- Chlorophyll b (907 Da, Ligand)
- Chlorophyll c2 (608 Da, Ligand)
- Prasinophyte-specific lhc protein (lhcp) complex (Complex from Ostreococcus tauri)
- Chlorophyll a (893 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaenyl]cyclohex-3-en-1-ol (570 Da, Ligand)
- (3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-1-[(1~{s},4~{s})-2,2-dimethyl-6-methylidene-1,4-bis(oxidanyl)cyclohexyl]-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-2-one (600 Da, Ligand)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8hg5
Q-score: 0.486
Ishii A, Shan J, Sheng X, Kim E, Watanabe A, Yokono M, Noda C, Song C, Murata K, Liu Z, Minagawa J
eLife (2023) 12 [ Pubmed: 36951548 DOI: doi:10.7554/eLife.84488 ]
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- Cryo-em structure of the prasinophyte-specific light-harvesting complex (lhcp)from ostreococcus tauri (Complex from Ostreococcus tauri)
- Chlorophyll b (907 Da, Ligand)
- Chlorophyll c2 (608 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaenyl]cyclohex-3-en-1-ol (570 Da, Ligand)
- (3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-1-[(1~{s},4~{s})-2,2-dimethyl-6-methylidene-1,4-bis(oxidanyl)cyclohexyl]-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-2-one (600 Da, Ligand)
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8hg6
Q-score: 0.412
Ishii A, Shan J, Sheng X, Kim E, Watanabe A, Yokono M, Noda C, Song C, Murata K, Liu Z, Minagawa J
eLife (2023) 12 [ Pubmed: 36951548 DOI: doi:10.7554/eLife.84488 ]
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Ostreococcus tauri)
- Chlorophyll b (907 Da, Ligand)
- Chlorophyll c2 (608 Da, Ligand)
- Prasinophyte-specific lhc protein (lhcp) complex (Complex from Ostreococcus tauri)
- Chlorophyll a (893 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaenyl]cyclohex-3-en-1-ol (570 Da, Ligand)
- (3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-1-[(1~{s},4~{s})-2,2-dimethyl-6-methylidene-1,4-bis(oxidanyl)cyclohexyl]-3,7,12,16-tetramethyl-18-[(1~{r},4~{r})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-3,5,7,9,11,13,15,17-octaen-2-one (600 Da, Ligand)