Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pk8
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 8pk9
Q-score: 0.661
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex from Homo sapiens)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8pka
Q-score: 0.624
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schunemann V, Murphy BJ, Lill R
Nat Commun (2024) 15 pp. 3269-3269 [ DOI: doi:10.1038/s41467-024-47310-8 Pubmed: 38627381 ]
- Water (18 Da, Ligand)
- Cysteine desulfurase, lyr motif-containing protein 4 and iron-sulfur cluster assembly enzyme iscu (Complex from Homo sapiens)
- Iron-bound human nfs1-isd11-acp1-iscu2-fxn (fe-niaux) complex during persulfide transfer (Complex)
- Cysteine desulfurase (44 kDa, Protein from Homo sapiens)
- Acyl carrier protein (Complex from Escherichia coli BL21(DE3))
- Fe (ii) ion (55 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
SARS-CoV-2 S 2P trimer in complex with monovalent DARPin R2 (State 1) - Composite Map
Rothenberger S, Hurdiss DL, Walser M, Malvezzi F, Mayor J, Ryter S, Moreno H, Liechti N, Bosshart A, Iss C, Calabro V, Cornelius A, Hospodarsch T, Neculcea A, Looser T, Schlegel A, Fontaine S, Villemagne D, Paladino M, Schiegg D, Mangold S, Reichen C, Radom F, Kaufmann Y, Schaible D, Schlegel I, Zitt C, Sigrist G, Straumann M, Wolter J, Comby M, Sacarcelik F, Drulyte I, Lyoo H, Wang C, Li W, Du W, Binz HK, Herrup R, Lusvarghi S, Neerukonda SN, Vassell R, Wang W, Adler JM, Eschke K, Nascimento M, Abdelgawad A, Gruber AD, Bushe J, Kershaw O, Knutson CG, Balavenkatraman KK, Ramanathan K, Wyler E, Teixeira Alves LG, Lewis S, Watson R, Haeuptle MA, Zurcher A, Dawson KM, Steiner D, Weiss CD, Amstutz P, van Kuppeveld FJM, Stumpp MT, Bosch BJ, Engler O, Trimpert J
Nat Biotechnol (2022) [ Pubmed: 35864170 DOI: doi:10.1038/s41587-022-01382-3 ]
- Monovalent darpin r2 (Protein from synthetic construct)
- Sars-cov-2 spike glycoprotein (Complex from Severe acute respiratory syndrome-related coronavirus)
- Monovalent darpin r2 (Complex from Synthetic construct)
- Sars-cov-2 s 2p trimer in complex with monovalent darpin r2 (state 1) (464 kDa, Complex)
- Sars-cov-2 spike glycoprotein (Protein from Severe acute respiratory syndrome-related coronavirus)
SARS-CoV-2 S 2P trimer in complex with monovalent DARPin R2 (State 2)
Rothenberger S, Hurdiss DL, Walser M, Malvezzi F, Mayor J, Ryter S, Moreno H, Liechti N, Bosshart A, Iss C, Calabro V, Cornelius A, Hospodarsch T, Neculcea A, Looser T, Schlegel A, Fontaine S, Villemagne D, Paladino M, Schiegg D, Mangold S, Reichen C, Radom F, Kaufmann Y, Schaible D, Schlegel I, Zitt C, Sigrist G, Straumann M, Wolter J, Comby M, Sacarcelik F, Drulyte I, Lyoo H, Wang C, Li W, Du W, Binz HK, Herrup R, Lusvarghi S, Neerukonda SN, Vassell R, Wang W, Adler JM, Eschke K, Nascimento M, Abdelgawad A, Gruber AD, Bushe J, Kershaw O, Knutson CG, Balavenkatraman KK, Ramanathan K, Wyler E, Teixeira Alves LG, Lewis S, Watson R, Haeuptle MA, Zurcher A, Dawson KM, Steiner D, Weiss CD, Amstutz P, van Kuppeveld FJM, Stumpp MT, Bosch BJ, Engler O, Trimpert J
Nat Biotechnol (2022) [ Pubmed: 35864170 DOI: doi:10.1038/s41587-022-01382-3 ]
- Monovalent darpin r2 (Protein from synthetic construct)
- Sars-cov-2 spike glycoprotein (Complex from Severe acute respiratory syndrome-related coronavirus)
- Sars-cov-2 s 2p trimer in complex with monovalent darpin r2 (state 2) (464 kDa, Complex)
- Monovalent darpin r2 (Complex from synthetic construct)
- Sars-cov-2 spike glycoprotein (Protein from Severe acute respiratory syndrome-related coronavirus)
SARS-CoV-2 S 2P trimer in complex with monovalent DARPin R2 (State 1) - Consensus Map
Rothenberger S, Hurdiss DL, Walser M, Malvezzi F, Mayor J, Ryter S, Moreno H, Liechti N, Bosshart A, Iss C, Calabro V, Cornelius A, Hospodarsch T, Neculcea A, Looser T, Schlegel A, Fontaine S, Villemagne D, Paladino M, Schiegg D, Mangold S, Reichen C, Radom F, Kaufmann Y, Schaible D, Schlegel I, Zitt C, Sigrist G, Straumann M, Wolter J, Comby M, Sacarcelik F, Drulyte I, Lyoo H, Wang C, Li W, Du W, Binz HK, Herrup R, Lusvarghi S, Neerukonda SN, Vassell R, Wang W, Adler JM, Eschke K, Nascimento M, Abdelgawad A, Gruber AD, Bushe J, Kershaw O, Knutson CG, Balavenkatraman KK, Ramanathan K, Wyler E, Teixeira Alves LG, Lewis S, Watson R, Haeuptle MA, Zurcher A, Dawson KM, Steiner D, Weiss CD, Amstutz P, van Kuppeveld FJM, Stumpp MT, Bosch BJ, Engler O, Trimpert J
Nat Biotechnol (2022) 40 pp. 1845-1854 [ Pubmed: 35864170 DOI: doi:10.1038/s41587-022-01382-3 ]
- Monovalent darpin r2 (Protein from synthetic construct)
- Sars-cov-2 spike glycoprotein (Complex from Severe acute respiratory syndrome-related coronavirus)
- Sars-cov-2 s 2p trimer in complex with monovalent darpin r2 (464 kDa, Complex)
- Monovalent darpin r2 (Complex from synthetic construct)
- Sars-cov-2 spike glycoprotein (Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 S 2P trimer in complex with monovalent DARPin R2 (State 1) - Focused Refinement
Rothenberger S, Hurdiss DL, Walser M, Malvezzi F, Mayor J, Ryter S, Moreno H, Liechti N, Bosshart A, Iss C, Calabro V, Cornelius A, Hospodarsch T, Neculcea A, Looser T, Schlegel A, Fontaine S, Villemagne D, Paladino M, Schiegg D, Mangold S, Reichen C, Radom F, Kaufmann Y, Schaible D, Schlegel I, Zitt C, Sigrist G, Straumann M, Wolter J, Comby M, Sacarcelik F, Drulyte I, Lyoo H, Wang C, Li W, Du W, Binz HK, Herrup R, Lusvarghi S, Neerukonda SN, Vassell R, Wang W, Adler JM, Eschke K, Nascimento M, Abdelgawad A, Gruber AD, Bushe J, Kershaw O, Knutson CG, Balavenkatraman KK, Ramanathan K, Wyler E, Teixeira Alves LG, Lewis S, Watson R, Haeuptle MA, Zurcher A, Dawson KM, Steiner D, Weiss CD, Amstutz P, van Kuppeveld FJM, Stumpp MT, Bosch BJ, Engler O, Trimpert J
Nat Biotechnol (2022) 40 pp. 1845-1854 [ Pubmed: 35864170 DOI: doi:10.1038/s41587-022-01382-3 ]
- Monovalent darpin r2 (Protein from synthetic construct)
- Sars-cov-2 spike glycoprotein (Complex from Severe acute respiratory syndrome-related coronavirus)
- Sars-cov-2 s 2p trimer in complex with monovalent darpin r2 (464 kDa, Complex)
- Monovalent darpin r2 (Complex from synthetic construct)
- Sars-cov-2 spike glycoprotein (Protein from Severe acute respiratory syndrome coronavirus 2)
Active state of GluA1/2 in complex with TARP gamma 8, L-glutamate and CTZ
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7qhb
Q-score: 0.453
Herguedas B, Kohegyi BK, Dohrke JN, Watson JF, Zhang D, Ho H, Shaikh SA, Lape R, Krieger JM, Greger IH
Nat Commun (2022) 13 pp. 734-734 [ Pubmed: 35136046 DOI: doi:10.1038/s41467-022-28404-7 ]
- Cyclothiazide (389 Da, Ligand)
- Isoform flip of glutamate receptor 2 (96 kDa, Protein from Rattus norvegicus)
- Palmitoleic acid (254 Da, Ligand)
- Complex between glua1/2 ampa receptor and auxiliary subunit tarp gamma8 (490 kDa, Complex from Rattus norvegicus)
- (2r)-2,3-dihydroxypropyl (9z)-octadec-9-enoate (356 Da, Ligand)
- Glutamic acid (147 Da, Ligand)
- (2s)-2,3-dihydroxypropyl (7z)-hexadec-7-enoate (328 Da, Ligand)
- Voltage-dependent calcium channel gamma-8 subunit (43 kDa, Protein from Rattus norvegicus)
- Isoform flip of glutamate receptor 1 (102 kDa, Protein from Rattus norvegicus)
Desensitized state of GluA1/2 AMPA receptor in complex with TARP-gamma 8 (TMD-LBD)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7qhh
Q-score: 0.359
Herguedas B, Kohegyi BK, Dohrke JN, Watson JF, Zhang D, Ho H, Shaikh SA, Lape R, Krieger JM, Greger IH
Nat Commun (2022) 13 pp. 734-734 [ Pubmed: 35136046 DOI: doi:10.1038/s41467-022-28404-7 ]
- Isoform flip of glutamate receptor 2 (96 kDa, Protein from Rattus norvegicus)
- Palmitoleic acid (254 Da, Ligand)
- Complex between glua1/2 ampa receptor and auxiliary subunit tarp gamma8 (490 kDa, Complex from Rattus norvegicus)
- (2r)-2,3-dihydroxypropyl (9z)-octadec-9-enoate (356 Da, Ligand)
- Glutamic acid (147 Da, Ligand)
- (2s)-2,3-dihydroxypropyl (7z)-hexadec-7-enoate (328 Da, Ligand)
- Voltage-dependent calcium channel gamma-8 subunit (43 kDa, Protein from Rattus norvegicus)
- Isoform flip of glutamate receptor 1 (102 kDa, Protein from Rattus norvegicus)
Structure of human SGLT2-MAP17 complex bound with empagliflozin
Niu Y, Liu R, Guan C, Zhang Y, Chen Z, Hoerer S, Nar H, Chen L
Nature (2022) 601 pp. 280-284 [ Pubmed: 34880493 DOI: doi:10.1038/s41586-021-04212-9 ]
- Sodium/glucose cotransporter 2 (72 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Human soluble guanylate cyclase (Complex from Homo sapiens)
- Pdzk1-interacting protein 1 (5 kDa, Protein from Homo sapiens)
- (2s,3r,4r,5s,6r)-2-[4-chloranyl-3-[[4-[(3s)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol (450 Da, Ligand)