Cryo-EM structure of the TnpB-omegaRNA-target DNA ternary complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8h1j
Q-score: 0.453
Nakagawa R, Hirano H, Omura SN, Nety S, Kannan S, Altae-Tran H, Yao X, Sakaguchi Y, Ohira T, Wu WY, Nakayama H, Shuto Y, Tanaka T, Sano FK, Kusakizako T, Kise Y, Itoh Y, Dohmae N, van der Oost J, Suzuki T, Zhang F, Nureki O
Nature (2023) 616 pp. 390-397 [ Pubmed: 37020030 DOI: doi:10.1038/s41586-023-05933-9 ]
- Tnpb-omegarna-target dna ternary complex (Complex from Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)
- Non-target strand (10 kDa, DNA from Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)
- Zinc ion (65 Da, Ligand)
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)
- Target strand (10 kDa, DNA from Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)
- Omegarna (130-mer) (79 kDa, RNA from Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)
Cryo-EM structure of RNC-RAC complex in presence of Ssb from S. cerevisiae 2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7x34
Q-score: 0.285
Nat Commun (2022) 13 pp. 3410-3410 [ DOI: doi:10.1038/s41467-022-31127-4 Pubmed: 35701497 ]
- Rnc-rac complex in presence of ssb from s. cerevisiae 2 (Complex from Saccharomyces cerevisiae)
- Zuotin (10 kDa, Protein from Saccharomyces cerevisiae)
- Rna (130-mer) (42 kDa, RNA from Saccharomyces cerevisiae)
Cryo-EM structure of Cas12g ternary complex
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6xmg
Q-score: 0.165
Li Z, Zhang H, Xiao R, Han R, Chang L
Nat Chem Biol (2021) 17 pp. 387-393 [ DOI: doi:10.1038/s41589-020-00721-2 Pubmed: 33495647 ]
- Crisp-cas complex (Complex from metagenome)
- Rna (5'-r(p*up*up*ap*ap*up*gp*cp*gp*gp*up*ap*gp*up*up*up*ap*up*cp*ap*cp*ap*gp*up*u)-3') (7 kDa, RNA from metagenome)
- Crispr-cas (89 kDa, Protein from metagenome)
- Zinc ion (65 Da, Ligand)
- Rna (130-mer) (43 kDa, RNA from metagenome)
Kappel K, Zhang K, Su Z, Watkins AM, Kladwang W, Li S, Pintilie G, Topkar VV, Rangan R, Zheludev IN, Yesselman JD, Chiu W, Das R
Nat Methods (2020) 17 pp. 699-707 [ DOI: doi:10.1038/s41592-020-0878-9 Pubmed: 32616928 ]
- Rna (130-mer) (42 kDa, RNA from synthetic construct)
- Atp-ttr-3 with amp (42 kDa, Complex from synthetic construct)
Kappel K, Zhang K, Su Z, Watkins AM, Kladwang W, Li S, Pintilie G, Topkar VV, Rangan R, Zheludev IN, Yesselman JD, Chiu W, Das R
Nat Methods (2020) 17 pp. 699-707 [ DOI: doi:10.1038/s41592-020-0878-9 Pubmed: 32616928 ]
- Rna (130-mer) (42 kDa, RNA from synthetic construct)
- Apo atp-ttr-3 (42 kDa, Complex from synthetic construct)