The cryo-EM structure of E. coli CueR transcription activation complex
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 6ldi
Q-score: 0.471
Fang C, Philips SJ, Wu X, Chen K, Shi J, Shen L, Xu J, Feng Y, O'Halloran TV, Zhang Y
Nat Chem Biol (2021) 17 pp. 57-64 [ DOI: doi:10.1038/s41589-020-00653-x Pubmed: 32989300 ]
- Rna (5'-r(*cp*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli cuer transcription activation complex (507 kDa, Complex from Escherichia coli K-12)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Silver ion (107 Da, Ligand)
- Dna (50-mer) (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Hth-type transcriptional regulator cuer (15 kDa, Protein from Escherichia coli (strain K12))
cryo-EM structure of Escherichia coli Crl transcription activation complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6kj6
Q-score: 0.479
Xu J, Cui K, Shen L, Shi J, Li L, You L, Fang C, Zhao G, Feng Y, Yang B, Zhang Y
eLife (2019) 8 [ DOI: doi:10.7554/eLife.50928 Pubmed: 31846423 ]
- Rna (5'-r(*cp*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Escherichia coli (strain K12))
- Sigma factor-binding protein crl (16 kDa, Protein from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks))
- Dna (51-mer) (15 kDa, DNA from synthetic construct)
- Escherichia coli crl transcription activation complex(crl-tac) (440 kDa, Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (51-mer) (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
CryoEM structure of Escherichia coli sigmaE transcription initiation complex containing 5nt of RNA
Resolution: 4.02 Å
EM Method: Single-particle
Fitted PDBs: 6jbq
Q-score: 0.392
Fang C, Li L, Shen L, Shi J, Wang S, Feng Y, Zhang Y
Nucleic Acids Res (2019) 47 pp. 7094-7104 [ DOI: doi:10.1093/nar/gkz470 Pubmed: 31131408 ]
- Rna (5'-r(*cp*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna (48-mer) (14 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Ecf rna polymerase sigma-e factor (25 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Escherichia coli sigmae transcription initiation complex containing 5nt of rna (443 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (48-mer) (14 kDa, DNA from synthetic construct)