Structure of activated transcription complex Pol II-DSIF-PAF-SPT6 (Map A)
Vos SM, Farnung L, Boehning M, Wigge C, Linden A, Urlaub H, Cramer P
Nature (2018) 560 pp. 607-612 [ Pubmed: 30135578 DOI: doi:10.1038/s41586-018-0440-4 ]
- Cdc73 (1 kDa, Protein from Homo sapiens)
- Paf1,rna polymerase ii-associated factor 1 homolog,rna polymerase ii-associated factor 1 homolog (67 kDa, Protein from Homo sapiens)
- Leo1,leo1,rna polymerase-associated protein leo1 (84 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Rpb1 (217 kDa, Protein from Sus scrofa)
- Rna polymerase ii-dsif-paf-spt6 elongation complex (ec*) (1 MDa, Complex)
- Rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
- Nucleic acids (Complex from synthetic construct)
- Rna (14 kDa, RNA from synthetic construct)
- Rpb12 (7 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Ctr9,rna polymerase-associated protein ctr9 homolog,rna polymerase-associated protein ctr9 homolog (125 kDa, Protein from Homo sapiens)
- Template dna (14 kDa, DNA from synthetic construct)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- Rpb10 (7 kDa, Protein from Sus scrofa)
- Associated proteins (Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Transcription elongation factor spt4 (13 kDa, Protein from Homo sapiens)
- Transcription elongation factor spt6,transcription elongation factor spt6,transcription elongation factor spt6 (198 kDa, Protein from Homo sapiens)
- Non-template dna (14 kDa, DNA from synthetic construct)
- Wd repeat-containing protein 61 (33 kDa, Protein from Homo sapiens)
- Rna polymerase ii (Complex from Sus scrofa)
- Rpb11 (13 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit g (19 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit c (31 kDa, Protein from Sus scrofa)
Structure of mammalian RNA polymerase II elongation complex inhibited by Alpha-amanitin
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6exv
Q-score: 0.482
Liu X, Farnung L, Wigge C, Cramer P
J Biol Chem (2018) 293 pp. 7189-7194 [ Pubmed: 29550768 DOI: doi:10.1074/jbc.RA118.002545 ]
- Dna-directed rna polymerase ii subunit rpb3 (16 kDa, Protein from Pig)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Pig)
- Rna polymerase ii elongation complex (Complex from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Pig)
- Dna (36-mer) (13 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Mammalian rna polymerase ii elongation complex inhibited by alpha-amanitin (607 kDa, Complex)
- Alpha-amanitin (Complex from Amanita phalloides)
- Amatoxin (939 Da, Protein from Amanita phalloides)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (13 kDa, Protein from Pig)
- Rna (5'-r(p*cp*ap*up*ap*ap*ap*gp*ap*cp*cp*ap*gp*gp*c)-3') (6 kDa, RNA from synthetic construct)
- Nucleic acids (Complex from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Pig)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Pig)
- Dna-directed rna polymerase ii subunit rpb2 (133 kDa, Protein from Pig)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Pig)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Pig)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Pig)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (14 kDa, Protein from Pig)
- Dna (25-mer) (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Pig)