Negative stain EM structure of the human SAGA coactivator complex (TRRAP, core, splicing module)
Resolution: 19.09 Å
EM Method: Single-particle
Fitted PDBs: 7kts
Q-score: 0.048
Herbst DA, Esbin MN, Louder RK, Dugast-Darzacq C, Dailey GM, Fang Q, Darzacq X, Tjian R, Nogales E
Nat Struct Mol Biol (2021) 28 pp. 989-996 [ DOI: doi:10.1038/s41594-021-00682-7 Pubmed: 34811519 ]
- Staga complex 65 subunit gamma (Complex from Homo sapiens)
- Taf5-like rna polymerase ii p300/cbp-associated factor-associated factor 65 kda subunit 5l (66 kDa, Protein from Homo sapiens)
- Transcription initiation factor tfiid subunit 10 (21 kDa, Protein from Homo sapiens)
- Isoform 3 of transcription factor spt20 homolog (88 kDa, Protein from Homo sapiens)
- Human saga (1 MDa, Complex)
- Transcription initiation factor tfiid subunit 12 (17 kDa, Protein from Homo sapiens)
- Transcriptional adapter 1 (37 kDa, Protein from Homo sapiens)
- Transcription initiation factor tfiid subunit 9b (27 kDa, Protein from Homo sapiens)
- Taf6-like rna polymerase ii p300/cbp-associated factor-associated factor 65 kda subunit 6l,taf6-like rna polymerase ii p300/cbp-associated factor-associated factor 65 kda subunit 6l,taf6-like rna polymerase ii p300/cbp-associated factor-associated factor 65 kda subunit 6l (62 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Transformation/transcription domain-associated protein,transformation/transcription domain-associated protein,transformation/transcription domain-associated protein,transformation/transcription domain-associated protein,transformation/transcription domain-associated protein (419 kDa, Protein from Homo sapiens)
- Human saga (Complex from Homo sapiens)
- Transcription initiation protein spt3 homolog,transcription initiation protein spt3 homolog,transcription initiation protein spt3 homolog (35 kDa, Protein from Homo sapiens)
- Staga complex 65 subunit gamma, dhaa,staga complex 65 subunit gamma,staga complex 65 subunit gamma, dhaa,staga complex 65 subunit gamma,staga complex 65 subunit gamma, dhaa (83 kDa, Protein from unclassified Rhodococcus)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Ataxin-7 (95 kDa, Protein from Homo sapiens)
Mediator-RNA Polymerase II Pre-Initiation Complex Delta TFIIE-IIH (Focused refinement)
Robinson PJ, Trnka MJ, Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD
Cell (2016) 166 pp. 1411-1422.e16 [ DOI: doi:10.1016/j.cell.2016.08.050 Pubmed: 27610567 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 19 (Protein from yeast)
- Transcription initiation factor iif subunit gamma (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb7 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 5 (Protein from yeast)
- Tata-box-binding protein (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 21 (Protein from yeast)
- Transcription initiation factor iib (Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iia large subunit (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 17 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 18 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb1 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 10 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 31 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 16 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 8 (Protein from yeast)
- Mediator-rna polymerase ii pre-initiation complex delta tfiie-iih (focused refinement) (1 MDa, Complex)
- Mediator of rna polymerase ii transcription subunit 4 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 7 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 3 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 15 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 22 (Protein from yeast)
- Transcription initiation factor iia subunit 2 (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 6 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 11 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb3 (Protein from yeast)
- Transcription initiation factor iif subunit beta (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 9 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb11 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 14 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 20 (Protein from yeast)
- 108 bp his4 promoter fragment (DNA from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit alpha (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb9 (Protein from yeast)
Electron cryo-microscopy of a transcribing Pol IIp-Capping Enzyme complex
Martinez-Rucobo FW, Kohler R, van de Waterbeemd M, Heck AJR, Hemann M, Herzog F, Stark H, Cramer P
Mol Cell (2015) 58 pp. 1079-1089 [ Pubmed: 25959396 DOI: doi:10.1016/j.molcel.2015.04.004 ]
- Transcribing pol iip-capping enzyme complex from yeast (720 kDa, Sample)
- Dna-directed rna polymerase ii (520 kDa, Protein from Saccharomyces cerevisiae)
- Mrna-capping enzyme (180 kDa, Protein from Saccharomyces cerevisiae)
- Dna-rna scaffold (20 kDa, Macromolecule from Saccharomyces cerevisiae)
Architecture of an RNA polymerase II transcription pre-initiation complex
Murakami K, Elmlund H, Kalisman N, Bushnell DA, Adams CM, Azubel M, Elmlund D, Levi-Kalisman Y, Liu X, Levitt M, Kornberg RD
Science (2013) 342 pp. 1238724-1238724 [ DOI: doi:10.1126/science.1238724 Pubmed: 24072820 ]
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Wu YM, Chang JW, Wang CH, Lin YC, Wu PL, Huang SH, Chang CC, Hu X, Gnatt A, Chang WH
EMBO J (2012) 31 pp. 3575-3587 [ Pubmed: 22850672 DOI: doi:10.1038/emboj.2012.205 ]
- Bovine rna polymerase ii elongation complex (520 kDa, Sample)
- Rna polymerase ii elongation complex (520 kDa, Protein from Bos taurus)
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Wu YM, Chang JW, Wang CH, Lin YC, Wu PL, Huang SH, Chang CC, Hu X, Gnatt A, Chang WH
EMBO J (2012) 31 pp. 3575-3587 [ Pubmed: 22850672 DOI: doi:10.1038/emboj.2012.205 ]
- Bovine rna polymerase ii with gdown1 (560 kDa, Sample)
- Rna polymerase ii complex (560 kDa, Protein from Bos taurus)
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Wu YM, Chang JW, Wang CH, Lin YC, Wu PL, Huang SH, Chang CC, Hu X, Gnatt A, Chang WH
EMBO J (2012) 31 pp. 3575-3587 [ Pubmed: 22850672 DOI: doi:10.1038/emboj.2012.205 ]
- Rna polymerase ii elongation complex (620 kDa, Protein from Bos taurus)
- Bovine rna polymerase ii complex (620 kDa, Sample)
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM
Wu YM, Chang JW, Wang CH, Lin YC, Wu PL, Huang SH, Chang CC, Hu X, Gnatt A, Chang WH
EMBO J (2012) 31 pp. 3575-3587 [ Pubmed: 22850672 DOI: doi:10.1038/emboj.2012.205 ]
- Bovine rna polymerase ii elongation complex with gdown1 (560 kDa, Sample)
- Rna polymerase ii elongation complex (560 kDa, Protein from Bos taurus)
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
Resolution: 16.0 Å
EM Method: Single-particle
Fitted PDBs: 3j1n, 3j1o
Q-score: 0.022
Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ
Structure (2012) 20 pp. 899-910 [ DOI: doi:10.1016/j.str.2012.02.023 Pubmed: 22579255 ]
- Tfiif (150 kDa, Protein from Saccharomyces cerevisiae)
- Complex of rna polymerase ii, mediator head module, tfiif, tfiib, tbp, and promoter dna (900 kDa, Sample)
- Head module (240 kDa, Protein from Saccharomyces cerevisiae)
- Rna polymerase ii (540 kDa, Protein from Saccharomyces cerevisiae)