Electron Microscopy Data Bank

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Structure of paused transcription complex Pol II-DSIF-NELF

Release date: Sept. 5, 2018
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6gml
Q-score: 0.34

Vos SM, Farnung L, Urlaub H, Cramer P

Nature (2018) 560 pp. 601-606 [ DOI: doi:10.1038/s41586-018-0442-2 Pubmed: 30135580 ]

Sample:

Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
Negative elongation factor (nelf) (Complex from Homo sapiens)
Negative elongation factor a (57 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
Negative elongation factor e,negative elongation factor e (45 kDa, Protein from Homo sapiens)
Hiv-1 pause transcription scaffold (Complex from Human immunodeficiency virus 1)
Rna polymerase ii subunit c (30 kDa, Protein from Sus scrofa)
Transcription elongation factor spt4 (13 kDa, Protein from Homo sapiens)
Rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
Sus scrofa rna polymerase ii (Complex from Sus scrofa)
Rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa)
Drb sensitivity inducing factor (dsif) (Complex from Human immunodeficiency virus 1)
Negative elongation factor c/d (64 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
Tar rna (14 kDa, RNA from Human immunodeficiency virus 1)
Rna polymerase ii subunit g (19 kDa, Protein from Sus scrofa)
Magnesium ion (24 Da, Ligand)
Uncharacterized protein (7 kDa, Protein from Sus scrofa)
Zinc ion (65 Da, Ligand)
Template dna (14 kDa, DNA from Human immunodeficiency virus 1)
Rna polymerase ii elongation complex bound to dsif and nelf (927 kDa, Complex)
Uncharacterized protein (7 kDa, Protein from Sus scrofa)
Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa)
Non-template dna (14 kDa, DNA from Human immunodeficiency virus 1)
Negative elongation factor b,negative elongation factor b (64 kDa, Protein from Homo sapiens)
Uncharacterized protein (13 kDa, Protein from Sus scrofa)
Rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa)

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