Cryo-EM structure of CII-dependent transcription activation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8igr
Q-score: 0.52
Zhao M, Gao B, Wen A, Feng Y, Lu YQ
Structure (2023) 31 pp. 968- [ DOI: doi:10.1016/j.str.2023.05.008 Pubmed: 37269829 ]
- Cii-dependent transcription activation complex (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Nontemplate strand dna (26 kDa, DNA from Escherichia coli (strain K12))
- Template strand dna (26 kDa, DNA from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Transcriptional activator ii (11 kDa, Protein from Escherichia phage Lambda)
Cryo-EM structure of RNAP-promoter open complex at lambda promoter PRE
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8igs
Q-score: 0.469
Zhao M, Gao B, Wen A, Feng Y, Lu YQ
Structure (2023) 31 pp. 968- [ DOI: doi:10.1016/j.str.2023.05.008 Pubmed: 37269829 ]
- Zinc ion (65 Da, Ligand)
- Rnap-promoter open complex (Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Non-template strand dna (26 kDa, DNA from Escherichia coli (strain K12))
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Template strand dna (26 kDa, DNA from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
CryoEM structure of sigma appropriation complex
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 6k4y
Q-score: 0.364
Shi J, Wen A, Zhao M, You L, Zhang Y, Feng Y
Nucleic Acids Res (2019) 47 pp. 9423-9432 [ DOI: doi:10.1093/nar/gkz682 Pubmed: 31392983 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli K-12)
- Dna (60-mer) (18 kDa, DNA from Enterobacteria phage T4)
- Zinc ion (65 Da, Ligand)
- 10 kda anti-sigma factor (12 kDa, Protein from Enterobacteria phage T4)
- Middle transcription regulatory protein mota (23 kDa, Protein from Enterobacteria phage T4)
- Sigma appropriation complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (60-mer) (18 kDa, DNA from Enterobacteria phage T4)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)