F. tularensis RNAPs70-(MglA-SspA)-iglA DNA complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 6wmr
Q-score: 0.466
Travis BA, Ramsey KM, Prezioso SM, Tallo T, Wandzilak JM, Hsu A, Borgnia M, Bartesaghi A, Dove SL, Brennan RG, Schumacher MA
Mol Cell (2021) 81 pp. 139- [ Pubmed: 33217319 DOI: doi:10.1016/j.molcel.2020.10.035 ]
- Dna-directed rna polymerase subunit beta' (157 kDa, Protein from Francisella tularensis subsp. holarctica (strain LVS))
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (67 kDa, Protein from Francisella tularensis subsp. holarctica LVS)
- Stringent starvation protein a (24 kDa, Protein from Francisella tularensis subsp. holarctica LVS)
- Dna t-strand (7 kDa, DNA from Francisella tularensis subsp. holarctica LVS)
- Francisella rnaps70-(mgla-sspa)-igla complex (Complex from Francisella tularensis subsp. holarctica LVS)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Francisella tularensis subsp. holarctica (strain LVS))
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Francisella tularensis subsp. holarctica (strain LVS))
- Dna t-strand downstream (3 kDa, DNA from Francisella tularensis subsp. holarctica LVS)
- Magnesium ion (24 Da, Ligand)
- Dna nt-strand downstream (3 kDa, DNA from Francisella tularensis subsp. holarctica LVS)
- Macrophage growth locus, subunit a (23 kDa, Protein from Francisella tularensis subsp. holarctica LVS)
- Dna-directed rna polymerase subunit alpha 2 (35 kDa, Protein from Francisella tularensis subsp. holarctica (strain LVS))
- Dna-directed rna polymerase subunit alpha 1 (35 kDa, Protein from Francisella tularensis subsp. holarctica (strain LVS))
- Dna nt-strand (10 kDa, DNA from Francisella tularensis subsp. holarctica LVS)
Resolution: 4.35 Å
EM Method: Single-particle
Fitted PDBs: 6pb4
Q-score: 0.224
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- The e. coli class-ii cap-dependent transcription activation complex with de novo rna transcript at the state 2 (550 kDa, Complex from Escherichia coli)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha, dna-directed rna polymerase subunit beta, dna-directed rna polymerase subunit beta', dna-directed rna polymerase subunit omega, rna polymerase sigma factor rpod, camp-activated global transcriptional regulator crp (Complex from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture
Resolution: 4.52 Å
EM Method: Single-particle
Fitted PDBs: 6pb5
Q-score: 0.082
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- The e coli class-ii cap-dependent transcription activation complex at the state 1 (550 kDa, Complex from Escherichia coli)
The E. coli class-II CAP-dependent transcription activation complex at the state 2
Resolution: 4.29 Å
EM Method: Single-particle
Fitted PDBs: 6pb6
Q-score: 0.223
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna (Complex)
- Zinc ion (65 Da, Ligand)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha, dna-directed rna polymerase subunit beta, dna-directed rna polymerase subunit beta', dna-directed rna polymerase subunit omega, rna polymerase sigma factor rpod, camp-activated global transcriptional regulator crp (Complex from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- The e. coli class-ii cap-dependent transcription activation complex at the state 2 (550 kDa, Complex from Escherichia coli)