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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"RPB7" AND author_name:"Grob" AND author_name:"Kassube"

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EMD-48071

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Cryo-EM structure of Human RNA polymerase II Elongation Complex in an Intermediate Translocation State

Release date: March 5, 2025
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9ehz

Ariza AJF, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025) [ Pubmed: 39975028 DOI: doi:10.1101/2025.01.29.634372 ]

Sample:

Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
Template dna, nucleic acid scaffold (8 kDa, DNA from Homo sapiens)
Rna, nucleic acid scaffold (6 kDa, RNA from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
Non-template dna, nucleic acid scaffold (13 kDa, DNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
Free stalled human rna polymerase ii elongation complex in an intermediate translocation state (430 kDa, Complex from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)

[Show more]

EMD-48073

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Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the absence of nucleotide

Release date: March 5, 2025
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ei1

Ariza AJF, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025) [ Pubmed: 39975028 DOI: doi:10.1101/2025.01.29.634372 ]

Sample:

Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
Template dna, nucleic acid scaffold (8 kDa, DNA from Homo sapiens)
Zinc ion (65 Da, Ligand)
Stalled human rna polymerase ii elongation complex bound to an apo recql5 helicase (490 kDa, Complex from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
Rna, nucleic acid scaffold (6 kDa, RNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
Atp-dependent dna helicase q5 (108 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
Non-template dna, nucleic acid scaffold (13 kDa, DNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)

[Show more]

EMD-48075

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Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of AMPPNP

Release date: March 5, 2025
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ei3

Ariza AJF, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025) [ Pubmed: 39975028 DOI: doi:10.1101/2025.01.29.634372 ]

Sample:

Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
Atp-dependent dna helicase q5 (109 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Template dna, nucleic acid scaffold (8 kDa, DNA from Homo sapiens)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
Rna, nucleic acid scaffold (6 kDa, RNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
Non-template dna, nucleic acid scaffold (13 kDa, DNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
Stalled human rna polymerase ii elongation complex bound to an amppnp-bound recql5 helicase (490 kDa, Complex from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)

[Show more]

EMD-48076

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Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP

Release date: March 5, 2025
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 9ei4

Ariza AJF, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025) [ Pubmed: 39975028 DOI: doi:10.1101/2025.01.29.634372 ]

Sample:

Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
Atp-dependent dna helicase q5 (109 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
Template dna, nucleic acid scaffold (8 kDa, DNA from Homo sapiens)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
Stalled human rna polymerase ii elongation complex bound to an adp-bound recql5 helicase (490 kDa, Complex from Homo sapiens)
Rna, nucleic acid scaffold (6 kDa, RNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
Non-template dna, nucleic acid scaffold (13 kDa, DNA from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)

[Show more]

EMD-2188

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human RNA polymerase II in complex with scAlu RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Rpb3 (30 kDa, Protein from Homo sapiens)
Scalu (38 kDa, RNA from Homo sapiens)
Rpb10 (10 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with scalu rna (520 kDa, Sample)
Rpb6 (10 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb5 (20 kDa, Protein from Homo sapiens)
Rpb8 (20 kDa, Protein from Homo sapiens)
Rpb11 (10 kDa, Protein from Homo sapiens)
Rpb2 (130 kDa, Protein from Homo sapiens)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
Rpb12 (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-2189

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human RNA polymerase II in complex with Alu RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Scalu (38 kDa, RNA from Homo sapiens)
Rpb10 (10 kDa, Protein from Homo sapiens)
Rpb2 (220 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Rpb8 (220 kDa, Protein from Homo sapiens)
Rpb5 (20 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb3 (30 kDa, Protein from Homo sapiens)
Rpb11 (10 kDa, Protein from Homo sapiens)
Rpb12 (10 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with alu rna (520 kDa, Sample)
Rpb6 (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-2190

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human RNA polymerase II in complex with AluRA RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Rpb10 (10 kDa, Protein from Homo sapiens)
Rpb8 (20 kDa, Protein from Homo sapiens)
Rpb2 (130 kDa, Protein from Homo sapiens)
Rpb12 (10 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with alura rna (520 kDa, Sample)
Rpb11 (10 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
Rpb3 (30 kDa, Protein from Homo sapiens)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Rpb5 (20 kDa, Protein from Homo sapiens)
Alura (47 kDa, RNA from Homo sapiens)
Rpb6 (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-2191

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human RNA polymerase II in complex with B2 RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Rpb10 (10 kDa, Protein from Homo sapiens)
Rpb8 (20 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Rpb2 (130 kDa, Protein from Homo sapiens)
Rpb5 (20 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
B2 rna (57 kDa, RNA from Mus musculus)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb3 (30 kDa, Protein from Homo sapiens)
Rpb11 (10 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with b2 rna (520 kDa, Sample)
Rpb12 (10 kDa, Protein from Homo sapiens)
Rpb6 (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-2192

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human RNA polymerase II in complex with B2 81-131 RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Rpb10 (10 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with b2 81-131 rna (520 kDa, Sample)
Rpb8 (20 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Rpb2 (130 kDa, Protein from Homo sapiens)
Rpb5 (20 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb3 (30 kDa, Protein from Homo sapiens)
Rpb11 (10 kDa, Protein from Homo sapiens)
Rpb12 (10 kDa, Protein from Homo sapiens)
B2 81-131 rna (16 kDa, RNA from Mus musculus)
Rpb6 (10 kDa, Protein from Homo sapiens)

[Show more]

EMD-2193

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human RNA polymerase II in complex with B1 RNA

Release date: Jan. 16, 2013
Resolution: 25.0 Å
EM Method: Single-particle

Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E

J Mol Biol (2013) 425 pp. 3639-3648 [ DOI: doi:10.1016/j.jmb.2012.08.024 Pubmed: 22954660 ]

Sample:

Rpb10 (10 kDa, Protein from Homo sapiens)
Rpb8 (20 kDa, Protein from Homo sapiens)
Rpb4 (20 kDa, Protein from Homo sapiens)
Rpb2 (130 kDa, Protein from Homo sapiens)
Human rna polymerase ii in complex with b1 rna (520 kDa, Sample)
Rpb5 (20 kDa, Protein from Homo sapiens)
Rpb9 (10 kDa, Protein from Homo sapiens)
Rpb1 (220 kDa, Protein from Homo sapiens)
Rpb7 (20 kDa, Protein from Homo sapiens)
Rpb3 (30 kDa, Protein from Homo sapiens)
B1 rna (45 kDa, RNA from Mus musculus)
Rpb11 (10 kDa, Protein from Homo sapiens)
Rpb12 (10 kDa, Protein from Homo sapiens)
Rpb6 (10 kDa, Protein from Homo sapiens)

[Show more]

Sort by: Download

Showing 1 - 10 of 11. Page 1 of 2

Display: Statistics

Download

Select database:

EMDB [11]

Format:

Select the script type:

Shell (Unix-based) Batch (Windows-based)

Preview first 10

Field: