Electron Microscopy Data Bank

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Structure of active transcription elongation complex Pol II-DSIF-ELL2-EAF1, composite map 2

Release date: July 14, 2021
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 7oky
Q-score: 0.387

Chen Y, Vos SM, Dienemann C, Ninov M, Urlaub H, Cramer P

Mol Cell (2021) 81 pp. 3386-3399.e10 [ Pubmed: 34265249 DOI: doi:10.1016/j.molcel.2021.06.019 ]

Sample:

Rna polymerase ii elongation factor ell2 (72 kDa, Protein from Homo sapiens)
Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
Transcription elongation factor spt4 (13 kDa, Protein from Homo sapiens)
Rna polymerase ii subunit d (20 kDa, Protein from Sus scrofa)
Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
Rna and dna (Complex from HIV whole-genome vector AA1305#18)
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa)
Rna (15 kDa, RNA from HIV whole-genome vector AA1305#18)
Template dna (14 kDa, DNA from HIV whole-genome vector AA1305#18)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Sus scrofa)
Elongation factor (Complex from Homo sapiens)
Rna_pol_l_2 domain-containing protein (13 kDa, Protein from Sus scrofa)
Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
Zinc ion (65 Da, Ligand)
Transcription elongation complex of rna polymerase ii with elongation factors dsif and ell2, eaf1 (Complex)
Ell-associated factor 1 (29 kDa, Protein from Homo sapiens)
Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
Elongation and association factor (Complex from Homo sapiens)
Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
Rna polymerase (Complex from Sus scrofa)
Dna-directed rna polymerase subunit beta (142 kDa, Protein from Sus scrofa)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
Non-template dna (14 kDa, DNA from HIV whole-genome vector AA1305#18)

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