the apo structure of human mTORC2 complex
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 7tzo
Q-score: 0.353
Yu Z, Chen J, Takagi E, Wang F, Saha B, Liu X, Joubert LM, Gleason CE, Jin M, Li C, Nowotny C, Agard D, Cheng Y, Pearce D
J Biol Chem (2022) 298 pp. 102288-102288 [ Pubmed: 35926713 DOI: doi:10.1016/j.jbc.2022.102288 ]
- Rapamycin-insensitive companion of mtor (193 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (37 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (302 kDa, Protein from Homo sapiens)
- Target of rapamycin complex 2 subunit mapkap1 (60 kDa, Protein from Homo sapiens)
- Apostate of mtorc2 complex, composed of mtor, rictor, mlst8 and msin1 (Complex from Homo sapiens)
cryo-EM structure of DEPTOR bound to human mTOR complex 2, overall refinement
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7pe7
Q-score: 0.401
Walchli M, Berneiser K, Mangia F, Imseng S, Craigie LM, Stuttfeld E, Hall MN, Maier T
eLife (2021) 10 [ Pubmed: 34519268 DOI: doi:10.7554/eLife.70871 ]
- Dep domain-containing mtor-interacting protein (46 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Acetyl group (44 Da, Ligand)
- Rapamycin-insensitive companion of mtor (192 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (291 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Target of rapamycin complex 2 subunit mapkap1 (59 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Mtorc2 in complex with its regulator deptor (1 MDa, Complex from Homo sapiens)
cryo-EM structure of DEPTOR bound to human mTOR complex 2, focussed on one protomer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pe8
Q-score: 0.448
Walchli M, Berneiser K, Mangia F, Imseng S, Craigie LM, Stuttfeld E, Hall MN, Maier T
eLife (2021) 10 [ Pubmed: 34519268 DOI: doi:10.7554/eLife.70871 ]
- Dep domain-containing mtor-interacting protein (46 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Acetyl group (44 Da, Ligand)
- Rapamycin-insensitive companion of mtor (192 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (291 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Target of rapamycin complex 2 subunit mapkap1 (59 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Mtorc2 in complex with its regulator deptor (1 MDa, Complex from Homo sapiens)
cryo-EM structure of DEPTOR bound to human mTOR complex 2, DEPt-bound subset local refinement
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7pe9
Q-score: 0.372
Walchli M, Berneiser K, Mangia F, Imseng S, Craigie LM, Stuttfeld E, Hall MN, Maier T
eLife (2021) 10 [ Pubmed: 34519268 DOI: doi:10.7554/eLife.70871 ]
- Dep domain-containing mtor-interacting protein (46 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Acetyl group (44 Da, Ligand)
- Rapamycin-insensitive companion of mtor (192 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (291 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Target of rapamycin complex 2 subunit mapkap1 (59 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Mtorc2 in complex with its regulator deptor (1 MDa, Complex from Homo sapiens)
cryo-EM structure of human mTOR complex 2, overall refinement
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6zwm
Q-score: 0.396
Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abc1251 Pubmed: 33158864 ]
- Serine/threonine-protein kinase mtor (289 kDa, Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (192 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Acetyl group (44 Da, Ligand)
- Human mtor complex 2 (1 MDa, Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Target of rapamycin complex 2 subunit mapkap1 (59 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
cryo-EM structure of human mTOR complex 2 in absence of ATP-gamma-S
Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abc1251 Pubmed: 33158864 ]
- Target of rapamycin complex 2 subunit mapkap1 (Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (Protein from Homo sapiens)
- Human mtor complex 2 (1 MDa, Complex from Homo sapiens)
Human mTOR complex 2 with additional density close to the mLST8
Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abc1251 Pubmed: 33158864 ]
- Target of rapamycin complex 2 subunit mapkap1 (Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (Protein from Homo sapiens)
- Human mtor complex 2 (1 MDa, Complex from Homo sapiens)
Human mTOR complex 2 with additional density close to the mLST8
Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abc1251 Pubmed: 33158864 ]
- Target of rapamycin complex 2 subunit mapkap1 (Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (Protein from Homo sapiens)
- Human mtor complex 2 (1 MDa, Complex from Homo sapiens)
cryo-EM structure of human mTOR complex 2, focused on one half
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zwo
Q-score: 0.527
Scaiola A, Mangia F, Imseng S, Boehringer D, Berneiser K, Shimobayashi M, Stuttfeld E, Hall MN, Ban N, Maier T
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abc1251 Pubmed: 33158864 ]
- Serine/threonine-protein kinase mtor (289 kDa, Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (192 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Acetyl group (44 Da, Ligand)
- Human mtor complex 2 (1 MDa, Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Target of rapamycin complex 2 subunit mapkap1 (59 kDa, Protein from Homo sapiens)
4.9 Angstrom Cryo-EM structure of human mTOR complex 2
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5zcs
Q-score: 0.164
Chen X, Liu M, Tian Y, Li J, Qi Y, Zhao D, Wu Z, Huang M, Wong CCL, Wang HW, Wang J, Yang H, Xu Y
Cell Res (2018) 28 pp. 518-528 [ DOI: doi:10.1038/s41422-018-0029-3 Pubmed: 29567957 ]
- Target of rapamycin complex 2 subunit mapkap1 (55 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase mtor (289 kDa, Protein from Homo sapiens)
- Rapamycin-insensitive companion of mtor (175 kDa, Protein from Homo sapiens)
- Human mtor complex 2 (1 GDa, Complex from Homo sapiens)