Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8rmr
Q-score: 0.326
Arragain B, Krischuns T, Pelosse M, Drncova P, Blackledge M, Naffakh N, Cusack S
Nat Commun (2024) 15 pp. 6910-6910 [ Pubmed: 39160148 DOI: doi:10.1038/s41467-024-51007-3 ]
- Asymmetric dimer composed of one flupola/h7n9-4m replicase and one flupola/h7n9-4m encapsidase, bridged by human anp32a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Acidic leucine-rich nuclear phosphoprotein 32 family member a (31 kDa, Protein from Homo sapiens)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Arragain B, Krischuns T, Pelosse M, Drncova P, Blackledge M, Naffakh N, Cusack S
Nat Commun (2024) 15 pp. 6910-6910 [ Pubmed: 39160148 DOI: doi:10.1038/s41467-024-51007-3 ]
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Heterotrimeric complex (from the asymmetric dimer composed of one flupola/h7n9-4m replicase and one flupola/h7n9-4m encapsidase, bridged by human anp32a) (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Acidic leucine-rich nuclear phosphoprotein 32 family member a (31 kDa, Protein from Homo sapiens)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8pm0
Q-score: 0.546
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ DOI: doi:10.1038/s41467-024-45205-2 Pubmed: 38316757 ]
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8pnp
Q-score: 0.611
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ DOI: doi:10.1038/s41467-024-45205-2 Pubmed: 38316757 ]
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8pnq
Q-score: 0.586
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ DOI: doi:10.1038/s41467-024-45205-2 Pubmed: 38316757 ]
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna pol ii ctd 6 repeats (site 1a/2a) (4 kDa, Protein from Homo sapiens)
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (89 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- Replication product (10 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Influenza A/H7N9 polymerase symmetric dimer bound to the promoter (PA K289A/C489R)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8poh
Q-score: 0.497
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun (2024) 15 pp. 1064-1064 [ DOI: doi:10.1038/s41467-024-45205-2 Pubmed: 38316757 ]
- 51-mer vrna loop (v51_mut_s) (16 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein, rna-directed rna polymerase catalytic subunit and polymerase basic protein 2 (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 51-mer vrna loop (v51_mut_s) (Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Influenza a/h7n9 polymerase in replicase-like conformation in pre-initiation state with pol ii ps5 ctd peptide mimic bound in site 1a/2a (Complex)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- Polymerase acidic protein (84 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (88 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))