Structure of the C. elegans Intron Lariat Spliceosome double-primed for disassembly (ILS'')
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ro1
Q-score: 0.373
Vorlander MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C
Nature (2024) 632 pp. 443-450 [ DOI: doi:10.1038/s41586-024-07741-1 Pubmed: 38925148 ]
- Intron lariat spliceosome (ils'') (Complex from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (36 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor spf27 (27 kDa, Protein from Caenorhabditis elegans)
- Replication stress response regulator sde2 (57 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein f (9 kDa, Protein from Caenorhabditis elegans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Probable small nuclear ribonucleoprotein e (10 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor syf1 (99 kDa, Protein from Caenorhabditis elegans)
- Cell division cycle 5-like protein (85 kDa, Protein from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (54 kDa, Protein from Caenorhabditis elegans)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Caenorhabditis elegans)
- Tr-type g domain-containing protein (110 kDa, Protein from Caenorhabditis elegans)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor syf-2 (27 kDa, Protein from Caenorhabditis elegans)
- Inositol hexakisphosphate (660 Da, Ligand)
- Intron lariat rna (10 kDa, RNA from Caenorhabditis elegans)
- Peptidyl-prolyl cis-trans isomerase e (8 kDa, Protein from Caenorhabditis elegans)
- U5 snrna (35 kDa, RNA from Caenorhabditis elegans)
- U6 snrna (32 kDa, RNA from Caenorhabditis elegans)
- Pre-mrna-processing factor 19 (53 kDa, Protein from Caenorhabditis elegans)
- Gcf c-terminal domain-containing protein (94 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein g (8 kDa, Protein from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Caenorhabditis elegans)
- U2 snrna (68 kDa, RNA from Caenorhabditis elegans)
- Protein isy-1 (31 kDa, Protein from Caenorhabditis elegans)
- Intron-binding protein aquarius (170 kDa, Protein from Caenorhabditis elegans)
- Wd_repeats_region domain-containing protein (65 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor rbm22 (45 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor 8 homolog (272 kDa, Protein from Caenorhabditis elegans)
- Protein bud31 homolog (17 kDa, Protein from Caenorhabditis elegans)
- Coiled-coil domain-containing protein 12 (8 kDa, Protein from Caenorhabditis elegans)
- Probable u2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Caenorhabditis elegans)
- Uncharacterized protein t27f2.1 (60 kDa, Protein from Caenorhabditis elegans)
- Pre-mrna-splicing factor atp-dependent rna helicase ddx-15 (84 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Caenorhabditis elegans)
- Cwf19-like protein 2 homolog (53 kDa, Protein from Caenorhabditis elegans)
- Rrm domain-containing protein (24 kDa, Protein from Caenorhabditis elegans)
- Magnesium ion (24 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase (18 kDa, Protein from Caenorhabditis elegans)
- Small nuclear ribonucleoprotein sm d3 (14 kDa, Protein from Caenorhabditis elegans)
- Cwf19-like protein 1 homolog (59 kDa, Protein from Caenorhabditis elegans)
- Tpr_region domain-containing protein (88 kDa, Protein from Caenorhabditis elegans)
- Septin and tuftelin-interacting protein 1 homolog (94 kDa, Protein from Caenorhabditis elegans)
- Probable small nuclear ribonucleoprotein-associated protein b (16 kDa, Protein from Caenorhabditis elegans)
Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qdv
Q-score: 0.278
Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K
Science (2019) 363 pp. 710-714 [ DOI: doi:10.1126/science.aaw5569 Pubmed: 30705154 ]
- Nf-kappa-b-activating protein (3 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (101 kDa, Protein from Homo sapiens)
- Human post-catalytic p complex spliceosome (3 MDa, Complex)
- Cactin (15 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
- Replication stress response regulator sde2 (4 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Snw domain-containing protein 1 (33 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (34 kDa, Protein from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
- Eukaryotic initiation factor 4a-iii, n-terminally processed (44 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (197 kDa, Protein from Homo sapiens)
- Protein mago nashi homolog 2 (16 kDa, Protein from Homo sapiens)
- Minx introns and exons (Complex from Human adenovirus 2)
- Protein fam32a (7 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein f (8 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (2 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (33 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
- Prkr-interacting protein 1 (14 kDa, Protein from Homo sapiens)
- Rna-binding protein 8a (10 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (Complex from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (10 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf2 (17 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (35 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (18 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Intron lariat: minx rna (27 kDa, RNA from Human adenovirus 2)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
- Ligated exons: minx mrna (4 kDa, RNA from Human adenovirus 2)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (9 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 17 (59 kDa, Protein from Homo sapiens)
- Human post-catalytic p complex spliceosome (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)