Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9jm0
Q-score: 0.492
Wang Y, Wang C, Guan Z, Cao J, Xu J, Wang S, Cui Y, Wang Q, Chen Y, Yin Y, Zhang D, Liu H, Sun M, Jin S, Tao P, Zou T
Cell Rep (2024) 43 pp. 114857-114857 [ DOI: doi:10.1016/j.celrep.2024.114857 Pubmed: 39395169 ]
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Retron ec86 reverse transcriptase (37 kDa, Protein from Escherichia coli)
- [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl [hydroxy-[[(2r,3s,4r,5s)-3,4,5-trihydroxyoxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (559 Da, Ligand)
- Rna (82-mer) (26 kDa, RNA from Escherichia coli)
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli)
- Nicotinamide (122 Da, Ligand)
- Retron ec86-effector fiber (Complex from Escherichia coli)
- Rna (5'-r(p*cp*gp*up*ap*ap*gp*gp*gp*up*gp*cp*gp*cp*a)-3') (4 kDa, RNA from Escherichia coli)
Retron-Eco1 filament with ADP-ribosylated Effector (local map with 1 segment)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8qbk
Q-score: 0.468
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl [hydroxy-[[(2r,3s,4r,5s)-3,4,5-trihydroxyoxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (559 Da, Ligand)
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1 in complex with adpr, containing rt-msr-msdna-effector complex (1 segment) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
Retron-Eco1 filament with inactive effector (E106A, 2 segments)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8qbl
Q-score: 0.387
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ DOI: doi:10.1016/j.molcel.2024.05.001 Pubmed: 38788717 ]
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1-e106a mutant containing rt-msr-msdna-effector complex (2 segments) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
Retron-Eco1 filament with ADP-ribosylated Effector (full map with 2 segments)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8qbm
Q-score: 0.365
Carabias A, Camara-Wilpert S, Mestre MR, Lopez-Mendez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G
Mol Cell (2024) 84 pp. 2185- [ Pubmed: 38788717 DOI: doi:10.1016/j.molcel.2024.05.001 ]
- Retron-eco1-a2 (4 kDa, RNA from Escherichia coli BL21(DE3))
- Retron ec86 putative ribosyltransferase/dna-binding protein (35 kDa, Protein from Escherichia coli BL21(DE3))
- Retron-eco1-msr (26 kDa, RNA from Escherichia coli BL21(DE3))
- Retron ec86 reverse transcriptase (39 kDa, Protein from Escherichia coli BL21(DE3))
- [(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl [hydroxy-[[(2r,3s,4r,5s)-3,4,5-trihydroxyoxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (559 Da, Ligand)
- Retron-eco1 msdna (26 kDa, DNA from Escherichia coli BL21(DE3))
- Filamentous assembly of retron-eco1 in complex with adpr, containing rt-msr-msdna-effector complex (2 segments) (21 MDa, Complex from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand from Escherichia coli BL21(DE3))