Cryo-EM map of the Saccharomyces cerevisiae PCNA clamp unloader Elg1-RFC complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8thb
Q-score: 0.489
Zheng F, Yao NY, Georgescu RE, Li H, O'Donnell ME
Sci Adv (2024) 10 pp. eadl1739-eadl1739 [ Pubmed: 38427736 DOI: doi:10.1126/sciadv.adl1739 ]
- Subunits rfc 2-5 of pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Elg1 subunit of pcna clamp unloader elg1-rfc (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (37 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Pcna clamp unloader elg1-rfc (Complex)
- Magnesium ion (24 Da, Ligand)
- Elg1 isoform 1 (91 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7thj
Q-score: 0.423
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Replication factor c (rfc), the eukaryotic clamp loader (336 kDa, Complex from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Rfc3 (RNA from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7tic
Q-score: 0.395
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch PhD BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Replication factor c (rfc), the eukaryotic clamp loader (336 kDa, Complex from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Rfc3 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7thv
Q-score: 0.406
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch PhD BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Pcna (Protein from Saccharomyces cerevisiae)
- Replication factor c (rfc), the eukaryotic clamp loader (336 kDa, Complex from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Rfc5 (RNA from Saccharomyces cerevisiae)
- Rfc3 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7tku
Q-score: 0.405
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch PhD BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Rfc3 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
- Structure of the yeast clamp loader (replication factor c rfc) bound to the open sliding clamp (proliferating cell nuclear antigen pcna) (336 kDa, Complex from Saccharomyces cerevisiae)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7tib
Q-score: 0.452
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch PhD BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Template (DNA from Saccharomyces cerevisiae)
- Replication factor c (rfc) bound to the open sliding clamp proliferating cell nuclear antigen (pcna) (336 kDa, Complex from Saccharomyces cerevisiae)
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Primer (DNA from Saccharomyces cerevisiae)
- Rfc3 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7tid
Q-score: 0.463
Gaubitz C, Liu X, Pajak J, Stone NP, Hayes JA, Demo G, Kelch PhD BA
eLife (2022) 11 [ Pubmed: 35179493 DOI: doi:10.7554/eLife.74175 ]
- Template (DNA from Saccharomyces cerevisiae)
- Replication factor c (rfc) bound to the open sliding clamp proliferating cell nuclear antigen (pcna) (336 kDa, Complex from Saccharomyces cerevisiae)
- Rfc5 (Protein from Saccharomyces cerevisiae)
- Pcna (Protein from Saccharomyces cerevisiae)
- Rfc1 (Protein from Saccharomyces cerevisiae)
- Primer (DNA from Saccharomyces cerevisiae)
- Rfc3 (Protein from Saccharomyces cerevisiae)
- Rfc4 (Protein from Saccharomyces cerevisiae)
- Rfc2 (Protein from Saccharomyces cerevisiae)
