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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

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offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Ribosomal protein L27" AND author_name:"Wilson DN" AND author_name:"Maviza"

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EMD-26486

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70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B

Release date: May 18, 2022
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7ug7
Q-score: 0.536

Wieland M, Holm M, Rundlet EJ, Morici M, Koller TO, Maviza TP, Pogorevc D, Osterman IA, Muller R, Blanchard SC, Wilson DN

PNAS (2022) 119 pp. e2114214119-e2114214119 [ Pubmed: 35500116 DOI: doi:10.1073/pnas.2114214119 ]

Sample:

30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
Guanosine-5'-diphosphate (443 Da, Ligand)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
Argyrin b (874 Da, Protein from Actinoplanes sp.)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
Elongation factor g (77 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
70s ribosome in an intermediate state of translocation bound to ef-g(gdp) and argyrinb (2 MDa, Complex from Escherichia coli)
30s ribosomal subunit (Complex from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli)
Trna-phe (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
1,4-diaminobutane (88 Da, Ligand)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
Spermidine (145 Da, Ligand)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
N-formylmethionine (177 Da, Ligand)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l1 (24 kDa, Protein from Escherichia coli)
50s ribosomal subunit (Complex from Escherichia coli)
23s rrna (941 kDa, RNA from Escherichia coli)
Trna-fmet (24 kDa, RNA from Escherichia coli)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
Ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
16s rrna (497 kDa, RNA from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
5s rrna (38 kDa, RNA from Escherichia coli)
Mrna (19 kDa, RNA from Escherichia coli)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)

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EMD-13058

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Cryo-EM structure of an Escherichia coli 70S ribosome in complex with elongation factor G and the antibiotic Argyrin B

Release date: May 11, 2022
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7otc
Q-score: 0.504

Wieland M, Holm M, Rundlet EJ, Morici M, Koller TO, Maviza TP, Pogorevc D, Osterman IA, Muller R, Blanchard SC, Wilson DN

PNAS (2022) 119 pp. e2114214119-e2114214119 [ Pubmed: 35500116 DOI: doi:10.1073/pnas.2114214119 ]

Sample:

50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli BL21(DE3))
Cryo-em structure of an escherichia coli 70s ribosome in complex with elongation factor g and the antibiotic argyrinb (Complex from Escherichia coli BL21(DE3))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli BL21(DE3))
Guanosine-5'-diphosphate (443 Da, Ligand)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli BL21(DE3))
16s ribosomal rna (497 kDa, RNA from Escherichia coli BL21(DE3))
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli BL21(DE3))
1,4-diaminobutane (88 Da, Ligand)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli BL21(DE3))
Adenosine-5'-triphosphate (507 Da, Ligand)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli BL21(DE3))
Spermidine (145 Da, Ligand)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli BL21(DE3))
Argyrin b (838 Da, Ligand)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli BL21(DE3))
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli BL21(DE3))
5s ribosomal rna (38 kDa, RNA from Escherichia coli BL21(DE3))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli BL21(DE3))
Elongation factor g (77 kDa, Protein from Escherichia coli BL21(DE3))
Zinc ion (65 Da, Ligand)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli BL21(DE3))
23s ribosomal rna (941 kDa, RNA from Escherichia coli BL21(DE3))
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli BL21(DE3))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli BL21(DE3))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli BL21(DE3))

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EMD-10709

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Tetracenomycin X bound to the human ribosome

Release date: July 8, 2020
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6y6x
Q-score: 0.546

Osterman IA, Wieland M, Maviza TP, Lashkevich KA, Lukianov DA, Komarova ES, Zakalyukina YV, Buschauer R, Shiriaev DI, Leyn SA, Zlamal JE, Biryukov MV, Skvortsov DA, Tashlitsky VN, Polshakov VI, Cheng J, Polikanov YS, Bogdanov AA, Osterman AL, Dmitriev SE, Beckmann R, Dontsova OA, Wilson DN, Sergiev PV

Nat Chem Biol (2020) 16 pp. 1071-1077 [ Pubmed: 32601485 DOI: doi:10.1038/s41589-020-0578-x ]

Sample:

60s ribosomal protein l19 (22 kDa, Protein from Homo sapiens)
60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
5s ribosomal rna (38 kDa, RNA from Homo sapiens)
28s ribosomal rna (1 MDa, RNA from Homo sapiens)
60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
60s ribosomal protein l34 (12 kDa, Protein from Homo sapiens)
60s ribosomal protein l14 (16 kDa, Protein from Homo sapiens)
60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
Tetracenomycin bound to the human ribosome (Complex from Homo sapiens)
60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
5.8s ribosomal rna (50 kDa, RNA from Homo sapiens)
60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
60s ribosomal protein l10-like (24 kDa, Protein from Homo sapiens)
Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Homo sapiens)
60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
60s ribosomal protein l7a (27 kDa, Protein from Homo sapiens)
60s ribosomal protein l4 (41 kDa, Protein from Homo sapiens)
60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
60s ribosomal protein l22 (11 kDa, Protein from Homo sapiens)
60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
60s ribosomal protein l31 (12 kDa, Protein from Homo sapiens)
60s ribosomal protein l26 (15 kDa, Protein from Homo sapiens)
60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
60s ribosomal protein l23 (13 kDa, Protein from Homo sapiens)
60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
60s ribosomal protein l8 (26 kDa, Protein from Homo sapiens)
60s ribosomal protein l23a (13 kDa, Protein from Homo sapiens)
60s ribosomal protein l6 (28 kDa, Protein from Homo sapiens)
60s ribosomal protein l7 (26 kDa, Protein from Homo sapiens)
60s ribosomal protein l29 (13 kDa, Protein from Homo sapiens)
60s ribosomal protein l3 (45 kDa, Protein from Homo sapiens)
60s ribosomal protein l24 (14 kDa, Protein from Homo sapiens)
60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
60s ribosomal protein l36 (11 kDa, Protein from Homo sapiens)
60s ribosomal protein l37 (10 kDa, Protein from Homo sapiens)
60s ribosomal protein l30 (10 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Tetracenomycin x (486 Da, Ligand)
60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
60s ribosomal protein l28 (14 kDa, Protein from Homo sapiens)
60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
60s ribosomal protein l17 (17 kDa, Protein from Homo sapiens)
60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)

[Show more]

EMD-10705

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Cryo-EM structure of an Escherichia coli 70S ribosome in complex with antibiotic TetracenomycinX

Release date: July 1, 2020
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 6y69
Q-score: 0.486

Osterman IA, Wieland M, Maviza TP, Lashkevich KA, Lukianov DA, Komarova ES, Zakalyukina YV, Buschauer R, Shiriaev DI, Leyn SA, Zlamal JE, Biryukov MV, Skvortsov DA, Tashlitsky VN, Polshakov VI, Cheng J, Polikanov YS, Bogdanov AA, Osterman AL, Dmitriev SE, Beckmann R, Dontsova OA, Wilson DN, Sergiev PV

Nat Chem Biol (2020) 16 pp. 1071-1077 [ Pubmed: 32601485 DOI: doi:10.1038/s41589-020-0578-x ]

Sample:

30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
Ribosome hibernation promoting factor (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
E-site trna (24 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (8 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
Cryo-em structure of the antibiotic tetracenomycinx bound to the escherichia coli 70s ribosome (2 MDa, Complex from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
Tetracenomycin x (486 Da, Ligand)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
16s ribosomal rna (498 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
23s ribosomal rna (941 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))

[Show more]

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EMDB [4]

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