Cryo-EM Structure of K63R Ubiquitin Mutant Ribosome under Oxidative Stress
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6xiq
Q-score: 0.33
Zhou Y, Kastritis PL, Dougherty SE, Bouvette J, Hsu AL, Burbaum L, Mosalaganti S, Pfeffer S, Hagen WJH, Forster F, Borgnia MJ, Vogel C, Beck M, Bartesaghi A, Silva GM
PNAS (2020) 117 pp. 22157-22166 [ Pubmed: 32855298 DOI: doi:10.1073/pnas.2005301117 ]
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae)
- Rpl8a isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae)
- K63 ubiquitinated ribosome (3 MDa, Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae)
- Rpl32 isoform 1 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- Rpl29 isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Rpl12a isoform 1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae)
- Rpl9a isoform 1 (21 kDa, Protein from Saccharomyces cerevisiae)
- Rps3 isoform 1 (26 kDa, Protein from Saccharomyces cerevisiae)
- Rps20 isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- Rps26b isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Rps25a isoform 1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Rps5p (25 kDa, Protein from Saccharomyces cerevisiae)
- Rps22a isoform 1 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Rpl38 isoform 1 (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
- Rps15 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Rpl11b isoform 1 (19 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (2 kDa, RNA from Saccharomyces cerevisiae)
- Rpl24a isoform 1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae)
- Rps2 isoform 1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Rpl4a isoform 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Rpl10 isoform 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- Transfer rna (24 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- Rpl3 isoform 1 (43 kDa, Protein from Saccharomyces cerevisiae)
- 35s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- Rps8a isoform 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- Rpl5 isoform 1 (33 kDa, Protein from Saccharomyces cerevisiae)
- Rpp0 isoform 1 (33 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- Rpl41a isoform 1 (3 kDa, Protein from Saccharomyces cerevisiae)
- Rpl1a isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Rps1a isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Rps28a isoform 1 (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6fti
Q-score: 0.207
Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon EC, Becker T, Forster F, Beckmann R
Science (2018) 360 pp. 215-219 [ Pubmed: 29519914 DOI: doi:10.1126/science.aar7899 ]
- Ul13 (23 kDa, Protein from Rabbit)
- Ribosomal protein l11 (19 kDa, Protein from Rabbit)
- 60s ribosomal protein l27 (15 kDa, Protein from Rabbit)
- Ribosomal protein l37a (10 kDa, Protein from Rabbit)
- 60s ribosomal protein l6 (28 kDa, Protein from Rabbit)
- Ul29 (14 kDa, Protein from Rabbit)
- Ul15 (16 kDa, Protein from Rabbit)
- Ul2 (26 kDa, Protein from Rabbit)
- Ul22 (17 kDa, Protein from Rabbit)
- Ribosomal protein l14 (16 kDa, Protein from Rabbit)
- Ul14 (21 kDa, Protein from Rabbit)
- Ul23 (13 kDa, Protein from Rabbit)
- El31 (12 kDa, Protein from Rabbit)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (80 kDa, Protein from Dog)
- Oligosaccharyltransferase (Complex from Canis lupus familiaris)
- El33 (12 kDa, Protein from Rabbit)
- El40 (6 kDa, Protein from Rabbit)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 (3 kDa, Protein from Dog)
- El8 (27 kDa, Protein from Rabbit)
- 60s ribosomal protein l41 (3 kDa, Protein from Rabbit)
- El32 (15 kDa, Protein from Rabbit)
- Ost48 (2 kDa, Protein from Dog)
- Unidentified tm (2 kDa, Protein from Dog)
- El13 (24 kDa, Protein from Rabbit)
- Ribosomal protein l24 (7 kDa, Protein from Rabbit)
- Ribosomal protein l4 (41 kDa, Protein from Rabbit)
- Ul30 (26 kDa, Protein from Rabbit)
- Ul3 (45 kDa, Protein from Rabbit)
- El38 (8 kDa, Protein from Rabbit)
- [(2~{s},3~{r},4~{r},5~{s},6~{r})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid (651 Da, Ligand)
- El34 (12 kDa, Protein from Rabbit)
- 5s ribosomal rna (38 kDa, RNA from Rabbit)
- Ribosomal protein l26 (15 kDa, Protein from Rabbit)
- 5.8s ribosomal rna (50 kDa, RNA from Rabbit)
- El20 (20 kDa, Protein from Rabbit)
- Rpn1 (6 kDa, Protein from Dog)
- El21 (18 kDa, Protein from Rabbit)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Rabbit)
- El28 (15 kDa, Protein from Rabbit)
- Oligosaccharyltransferase complex subunit ostc (13 kDa, Protein from Dog)
- El39 (6 kDa, Protein from Rabbit)
- Ribosomal protein l15 (24 kDa, Protein from Rabbit)
- El30 (10 kDa, Protein from Rabbit)
- Ul6 (21 kDa, Protein from Rabbit)
- 60s ribosomal protein l5 (34 kDa, Protein from Rabbit)
- Tmem258 (4 kDa, Protein from Dog)
- Magnesium ion (24 Da, Ligand)
- Ribosomal protein l10 (predicted) (24 kDa, Protein from Rabbit)
- 60s ribosomal protein l29 (8 kDa, Protein from Rabbit)
- Ribosomal protein l37 (10 kDa, Protein from Rabbit)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1,rpn1 (12 kDa, Protein from Dog)
- El22 (11 kDa, Protein from Rabbit)
- Protein transport protein sec61 subunit beta (3 kDa, Protein from Dog)
- Protein transport protein sec61 subunit alpha isoform 1 (50 kDa, Protein from Dog)
- 60s ribosomal protein l36 (11 kDa, Protein from Rabbit)
- Zinc ion (65 Da, Ligand)
- Mammalian oligosaccharyltransferase in complex with sec61 and the 80s ribosome (Complex)
- El19 (21 kDa, Protein from Rabbit)
- Ul14 (13 kDa, Protein from Rabbit)
- El42 (12 kDa, Protein from Rabbit)
- P-site trna (24 kDa, RNA from Rabbit)
- Dad1 (9 kDa, Protein from Dog)
- Ribosomal protein l12 (17 kDa, Protein from Rabbit)
- 28s rrna (1 MDa, RNA from Rabbit)
- Sec61 protein conducting channel (Complex from Canis lupus familiaris)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Dog)
- 60s ribosomal subunit (Complex from Oryctolagus cuniculus)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6ftj
Q-score: 0.174
Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon EC, Becker T, Forster F, Beckmann R
Science (2018) 360 pp. 215-219 [ Pubmed: 29519914 DOI: doi:10.1126/science.aar7899 ]
- Ul14 (13 kDa, Protein from Oryctolagus cuniculus)
- Ul13 (23 kDa, Protein from Oryctolagus cuniculus)
- Ul15 (16 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l10 (predicted) (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l12 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l24 (7 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l36 (11 kDa, Protein from Oryctolagus cuniculus)
- El13 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l5 (34 kDa, Protein from Oryctolagus cuniculus)
- Oligosaccharyltransferase (Complex from Canis lupus familiaris)
- El40 (6 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- Tmem258 (5 kDa, Protein from Canis lupus familiaris)
- Ul6 (21 kDa, Protein from Oryctolagus cuniculus)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1,rpn1 (17 kDa, Protein from Canis lupus familiaris)
- Ul30 (26 kDa, Protein from Oryctolagus cuniculus)
- Ost4 (3 kDa, Protein from Canis lupus familiaris)
- Ribosomal protein l4 (41 kDa, Protein from Oryctolagus cuniculus)
- Ul3 (45 kDa, Protein from Oryctolagus cuniculus)
- Mammalian oligosaccharyltransferase in complex with sec61 and the non-programmed 80s ribosome (Complex)
- Ribosomal protein l14 (16 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l6 (28 kDa, Protein from Oryctolagus cuniculus)
- 5s ribosomal rna (38 kDa, RNA from Oryctolagus cuniculus)
- El38 (8 kDa, Protein from Oryctolagus cuniculus)
- [(2~{s},3~{r},4~{r},5~{s},6~{r})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid (651 Da, Ligand)
- Ribosomal protein l37 (10 kDa, Protein from Oryctolagus cuniculus)
- El32 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- El39 (6 kDa, Protein from Oryctolagus cuniculus)
- Ul14 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Canis lupus familiaris)
- El33 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l37a (10 kDa, Protein from Oryctolagus cuniculus)
- El19 (21 kDa, Protein from Oryctolagus cuniculus)
- Rpn2 (6 kDa, Protein from Canis lupus familiaris)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (79 kDa, Protein from Canis lupus familiaris)
- Ul29 (14 kDa, Protein from Oryctolagus cuniculus)
- Ul22 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (8 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit alpha isoform 1 (47 kDa, Protein from Canis lupus familiaris)
- Ost48 (2 kDa, Protein from Canis lupus familiaris)
- Magnesium ion (24 Da, Ligand)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- El21 (18 kDa, Protein from Oryctolagus cuniculus)
- Ul8 (27 kDa, Protein from Oryctolagus cuniculus)
- El42 (12 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit beta (3 kDa, Protein from Canis lupus familiaris)
- El28 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l11 (19 kDa, Protein from Oryctolagus cuniculus)
- El30 (10 kDa, Protein from Oryctolagus cuniculus)
- Dad1 (8 kDa, Protein from Canis lupus familiaris)
- El31 (12 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Oligosaccharyltransferase complex subunit ostc (13 kDa, Protein from Canis lupus familiaris)
- Ul2 (26 kDa, Protein from Oryctolagus cuniculus)
- Sec61 protein conducting channel (Complex from Canis lupus familiaris)
- 60s ribosomal subunit (Complex from Oryctolagus cuniculus)
- El20 (20 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l22 (11 kDa, Protein from Oryctolagus cuniculus)
- Ul23 (13 kDa, Protein from Oryctolagus cuniculus)
- El34 (12 kDa, Protein from Oryctolagus cuniculus)
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- 5.8s ribosomal rna (50 kDa, RNA from Oryctolagus cuniculus)