Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 9f1c
Q-score: 0.371
Lentzsch AM, Yudin D, Gamerdinger M, Chandrasekar S, Rabl L, Scaiola A, Deuerling E, Ban N, Shan SO
Nature (2024) 633 pp. 718-724 [ DOI: doi:10.1038/s41586-024-07846-7 Pubmed: 39169182 ]
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- Human methionine aminopeptidase 1 (Complex from Homo sapiens)
- Small ribosomal subunit protein us13 (17 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein us5 (31 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein us11 (16 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein rack1 (35 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s19 (16 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el40 (14 kDa, Protein from Oryctolagus cuniculus)
- Human nata/e complex (Complex)
- 40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l36 (12 kDa, Protein from Oryctolagus cuniculus)
- S10_plectin domain-containing protein (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l22 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l37a (10 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l21 (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l9 (21 kDa, Protein from Oryctolagus cuniculus)
- Rabbit ribosome-nascent chain complex (Complex from Oryctolagus cuniculus)
- Ribosomal protein ul30 (29 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein ul2 (28 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3a (30 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l23en domain-containing protein (17 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein es21 (9 kDa, Protein from Oryctolagus cuniculus)
- Mrna (543 Da, RNA from Oryctolagus cuniculus)
- Unknown atom or ion (Ligand)
- 60s ribosomal protein l6 (33 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el28 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s8 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l31 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein es17 (15 kDa, Protein from Oryctolagus cuniculus)
- Spermidine (145 Da, Ligand)
- 40s ribosomal protein s29 (6 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
- Inositol hexakisphosphate (660 Da, Ligand)
- 40s ribosomal protein s9 (22 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l23 (14 kDa, Protein from Oryctolagus cuniculus)
- Spermine (202 Da, Ligand)
- Small ribosomal subunit protein us2 (32 kDa, Protein from Oryctolagus cuniculus)
- Nascent chain (2 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (26 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l32 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s7 (47 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l24 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s13 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l30 (12 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Oryctolagus cuniculus)
- Methionine aminopeptidase 1 (43 kDa, Protein from Homo sapiens)
- 40s ribosomal protein us19 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s27a (18 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein ul13 (23 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el13 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l7a (30 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l34 (13 kDa, Protein from Oryctolagus cuniculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 5.8s rrna (50 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el20 (20 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l19 (23 kDa, Protein from Oryctolagus cuniculus)
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el18 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l11 (20 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35a (12 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein us9 (19 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l39-like (6 kDa, Protein from Oryctolagus cuniculus)
- Nascent polypeptide-associated complex subunit alpha (25 kDa, Protein from Homo sapiens)
- Ribosomal protein l37 (11 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein el42 (12 kDa, Protein from Oryctolagus cuniculus)
- P-site trna (15 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l14 (23 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l38 (8 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
- 18s rrna (603 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s6 (28 kDa, Protein from Oryctolagus cuniculus)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l3 (46 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l10 (24 kDa, Protein from Oryctolagus cuniculus)
- N-alpha-acetyltransferase 15, nata auxiliary subunit (106 kDa, Protein from Homo sapiens)
- Ribosomal protein s5 (22 kDa, Protein from Oryctolagus cuniculus)
- N-alpha-acetyltransferase 10 (26 kDa, Protein from Homo sapiens)
- Human nata complex (Complex from Homo sapiens)
- 60s ribosomal protein l12 (17 kDa, Protein from Oryctolagus cuniculus)
- Transcription factor btf3 (17 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Nac heterodimer (Complex from Homo sapiens)
- 40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s acidic ribosomal protein p0 (34 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3 (31 kDa, Protein from Oryctolagus cuniculus)
- Small ribosomal subunit protein es26 (13 kDa, Protein from Oryctolagus cuniculus)
- Quaternary complex of a translating 80s ribosome, nac, metap1 and nata/e (3 MDa, Complex)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- Glutathione s-transferase class-mu 26 kda isozyme,n-alpha-acetyltransferase 50 (46 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul4 (46 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- 5s rrna (38 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
- Human nate with a gst tag (Complex from Homo sapiens)
- Large ribosomal subunit protein ul18 (34 kDa, Protein from Oryctolagus cuniculus)
Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1)
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5wyj
Q-score: 0.16
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (dhr1-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- Essential nuclear protein 1 (55 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- Utp20 (212 kDa, Protein from Baker's yeast)
- Rrna biogenesis protein rrp5 (193 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 5ets rna (225 kDa, RNA from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 10,utp10 (173 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)
Cryo-EM structure of the 90S small subunit pre-ribosome (Mtr4-depleted, Enp1-TAP)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5wyk
Q-score: 0.267
Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K
eLife (2017) 6 [ Pubmed: 28244370 DOI: doi:10.7554/eLife.22086 ]
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Baker's yeast)
- Utp5 (54 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (583 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- Helical domain protein (137 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 10 (200 kDa, Protein from Baker's yeast)
- Utp11 (21 kDa, Protein from Baker's yeast)
- Utp17 (76 kDa, Protein from Baker's yeast)
- Enp2 (60 kDa, Protein from Baker's yeast)
- Utp15 (43 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Baker's yeast)
- Periodic tryptophan protein 2 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Baker's yeast)
- Sof1 (41 kDa, Protein from Baker's yeast)
- Rrna-processing protein fcf1 (21 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Helical domain protein (84 kDa, Protein from Baker's yeast)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- Mpp10,u3 small nucleolar rna-associated protein mpp10 (54 kDa, Protein from Baker's yeast)
- 90s small subunit pre-ribosome (mtr4-depleted, enp1-tap) (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Baker's yeast)
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- Unassigned helices (87 kDa, Protein from Baker's yeast)
- Utp8 (60 kDa, Protein from Baker's yeast)
- Krr1 small subunit processome component (37 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- Utp7 (47 kDa, Protein from Baker's yeast)
- 5ets rna (225 kDa, RNA from Baker's yeast)
- Utp9 (48 kDa, Protein from Baker's yeast)
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Baker's yeast)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Baker's yeast)
- Nucleolar protein 58 (57 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- U3 rna (106 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- Nucleolar protein 56 (56 kDa, Protein from Baker's yeast)
- Utp4 (66 kDa, Protein from Baker's yeast)
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1A)
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 5lmn
Q-score: 0.418
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1a) (826 kDa, Complex)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s ribosomal rna (493 kDa, RNA from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5lmo
Q-score: 0.281
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Adenosine-5'-monophosphate (347 Da, Ligand)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Uridine-5'-monophosphate (324 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s-if1-if3-mrna pre-initiation complex (state-1b) (826 kDa, Complex)
- Guanosine-5'-monophosphate (363 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C)
Resolution: 5.35 Å
EM Method: Single-particle
Fitted PDBs: 5lmp
Q-score: 0.213
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1c) (826 kDa, Complex)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5lmq
Q-score: 0.326
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trna (24 kDa, RNA from Escherichia coli)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex, open form (state-2a) (849 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B)
Resolution: 4.45 Å
EM Method: Single-particle
Fitted PDBs: 5lmr
Q-score: 0.294
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2b) (849 kDa, Complex)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C)
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5lms
Q-score: 0.231
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2c) (849 kDa, Complex)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trnai (24 kDa, RNA from Escherichia coli)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- Zinc ion (65 Da, Ligand)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-3)
Resolution: 4.15 Å
EM Method: Single-particle
Fitted PDBs: 5lmt
Q-score: 0.329
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-3) (849 kDa, Complex)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- 16s ribosomal rna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)