Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1A)
Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 5lmn
Q-score: 0.418
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s ribosomal rna (493 kDa, RNA from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1a) (826 kDa, Complex)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5lmo
Q-score: 0.281
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Uridine-5'-monophosphate (324 Da, Ligand)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Guanosine-5'-monophosphate (363 Da, Ligand)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Adenosine-5'-monophosphate (347 Da, Ligand)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1b) (826 kDa, Complex)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C)
Resolution: 5.35 Å
EM Method: Single-particle
Fitted PDBs: 5lmp
Q-score: 0.213
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1c) (826 kDa, Complex)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5lmq
Q-score: 0.326
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trna (24 kDa, RNA from Escherichia coli)
- 30s-if1-if3-mrna-trna pre-initiation complex, open form (state-2a) (849 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B)
Resolution: 4.45 Å
EM Method: Single-particle
Fitted PDBs: 5lmr
Q-score: 0.294
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2b) (849 kDa, Complex)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C)
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5lms
Q-score: 0.231
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2c) (849 kDa, Complex)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trnai (24 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-3)
Resolution: 4.15 Å
EM Method: Single-particle
Fitted PDBs: 5lmt
Q-score: 0.329
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-3) (849 kDa, Complex)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Thermus thermophilus HB8)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 16s ribosomal rna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5lmu
Q-score: 0.355
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-4) (841 kDa, Complex)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 16s ribosomal rna (493 kDa, RNA from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
Structure of RelA bound to the 70S ribosome (overall map, class 1)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 5iqr
Q-score: 0.45
Brown A, Fernandez IS, Gordiyenko Y, Ramakrishnan V
Nature (2016) 534 pp. 277-280 [ Pubmed: 27279228 DOI: doi:10.1038/nature17675 ]
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- Lsu rrna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- Ssu rrna (497 kDa, RNA from Escherichia coli K-12)
- P-site fmet-trna(fmet) (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 5s rrna (38 kDa, RNA from Escherichia coli K-12)
- E-site trna(phe) (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- Paromomycin (615 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- Gtp pyrophosphokinase (83 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Mrna (3 kDa, RNA from synthetic construct)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- Methionine (149 Da, Ligand)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- A/t trna(phe) (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (25 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)