Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 8ygs
Q-score: 0.444
Huang Y, Song F, Zeng Y, Sun H, Sheng R, Wang X, Liu L, Luo G, Jiang Y, Chen Y, Zhang M, Zhang S, Gu Y, Yu H, Li S, Li T, Zheng Q, Ge S, Zhang J, Xia N
Nat Commun (2025) 16 pp. 838-838 [ Pubmed: 39833145 DOI: doi:10.1038/s41467-025-56114-3 ]
- Immune complex of rotavirus with 7h13 (Complex from Rotavirus A)
- Antibody 7h13 light chain (11 kDa, Protein from Mus musculus)
- Outer capsid protein vp4 (64 kDa, Protein from Rotavirus A)
- Antibody 7h13 heavy chain (12 kDa, Protein from Mus musculus)
- Fab 7h13 (Complex from Mus musculus)
- Outer capsid protein vp4 (64 kDa, Protein from Rotavirus A)
- Rotavirus vp4 (Complex from Rotavirus A)
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13 at Low concentration
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13-I54G mutant (left side)
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8ygt
Q-score: 0.559
Huang Y, Song F, Zeng Y, Sun H, Sheng R, Wang X, Liu L, Luo G, Jiang Y, Chen Y, Zhang M, Zhang S, Gu Y, Yu H, Li S, Li T, Zheng Q, Ge S, Zhang J, Xia N
Nat Commun (2025) 16 pp. 838-838 [ Pubmed: 39833145 DOI: doi:10.1038/s41467-025-56114-3 ]
- Immune complex of rotavirus with 7h13 (Complex from Rotavirus A)
- Antibody 7h13-i54g mutant heavy chain (12 kDa, Protein from Mus musculus)
- Fab 7h13 (Complex from Mus musculus)
- Antibody 7h13-i54g mutant light chain (11 kDa, Protein from Mus musculus)
- Outer capsid protein vp4 (64 kDa, Protein from Rotavirus A)
- Rotavirus vp4 (Complex from Rotavirus A)
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13-I54G mutant (right side)
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8ygu
Q-score: 0.526
Huang Y, Song F, Zeng Y, Sun H, Sheng R, Wang X, Liu L, Luo G, Jiang Y, Chen Y, Zhang M, Zhang S, Gu Y, Yu H, Li S, Li T, Zheng Q, Ge S, Zhang J, Xia N
Nat Commun (2025) 16 pp. 838-838 [ Pubmed: 39833145 DOI: doi:10.1038/s41467-025-56114-3 ]
- Outer capsid protein vp4 (87 kDa, Protein from Rotavirus A)
- Immune complex of rotavirus with 7h13-i54g mutant (Complex from Rotavirus A)
- Fab 7h13 (Complex from Rotavirus A)
- Antibody 7h13-i54g mutant heavy chain (12 kDa, Protein from Mus musculus)
- Antibody 7h13-i54g mutant light chain (11 kDa, Protein from Mus musculus)
- Outer capsid protein vp4 (64 kDa, Protein from Rotavirus A)
- Rotavirus vp4 (Complex from Rotavirus A)
Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Upright conformation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6wxe
Q-score: 0.472
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- Rotavirus vp4, vp6, vp7 assembly in the spike conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxf
Q-score: 0.306
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Rotavirus vp4, vp6, vp7 assembly in an intermediate confromattion (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Reversed conformation
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxg
Q-score: 0.509
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Rotavirus vp4, vp6, vp7 assembly in the foldback conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))