Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8qyv
Q-score: 0.324
Jalal ASB, Girvan P, Chua EYD, Liu L, Wang S, McCormack EA, Skehan MT, Knight CL, Rueda DS, Wigley DB
Mol Cell (2024) 84 pp. 3871- [ Pubmed: 39226902 DOI: doi:10.1016/j.molcel.2024.08.015 ]
- Helicase swr1 (174 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-like protein arp6 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Swr1-hexasome complex (Complex from Saccharomyces cerevisiae S288C)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ruvb-like protein 2 (51 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (118-mer) (36 kDa, DNA from synthetic construct)
- Dna (118-mer) (36 kDa, DNA from synthetic construct)
- Vacuolar protein sorting-associated protein 71 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Swr1-complex protein 5 (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ruvb-like protein 1 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Vacuolar protein sorting-associated protein 72 (90 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8qz0
Q-score: 0.293
Jalal ASB, Girvan P, Chua EYD, Liu L, Wang S, McCormack EA, Skehan MT, Knight CL, Rueda DS, Wigley DB
Mol Cell (2024) 84 pp. 3871- [ Pubmed: 39226902 DOI: doi:10.1016/j.molcel.2024.08.015 ]
- Swr1-hexasome-dimer complex (Complex from Saccharomyces cerevisiae S288C)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ruvb-like protein 2 (51 kDa, Protein from Saccharomyces cerevisiae S288C)
- Swr1-complex protein 5 (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ruvb-like protein 1 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Helicase swr1 (174 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (113-mer) (36 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2a.z (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Histone h2a.z-specific chaperone chz1 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Vacuolar protein sorting-associated protein 72 (90 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-like protein arp6 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Vacuolar protein sorting-associated protein 71 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (113-mer) (36 kDa, DNA from synthetic construct)
SWR1 lacking Swc5 subunit in complex with hexasome
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 9fbw
Q-score: 0.193
Jalal ASB, Girvan P, Chua EYD, Liu L, Wang S, McCormack EA, Skehan MT, Knight CL, Rueda DS, Wigley DB
Mol Cell (2024) 84 pp. 3871- [ Pubmed: 39226902 DOI: doi:10.1016/j.molcel.2024.08.015 ]
- Helicase swr1 (174 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Actin-like protein arp6 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ruvb-like protein 2 (51 kDa, Protein from Saccharomyces cerevisiae S288C)
- Vacuolar protein sorting-associated protein 71 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (112-mer) (34 kDa, DNA from synthetic construct)
- Dna (112-mer) (34 kDa, DNA from synthetic construct)
- Ruvb-like protein 1 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Zinc ion (65 Da, Ligand)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Vacuolar protein sorting-associated protein 72 (90 kDa, Protein from Saccharomyces cerevisiae S288C)
- Swr1 lacking swc5 subunit in complex with hexasome (Complex from Saccharomyces cerevisiae S288C)
CryoEM Structure INO80core Hexasome complex composite map state1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8oo7
Q-score: 0.556
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Histones (Complex from Homo sapiens)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (Complex from Synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
INO80 core bound to hexasome composite map of state 2
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8oop
Q-score: 0.554
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (Complex from Synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
CryoEM Structure INO80core Hexasome complex Rvb core refinement state2
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8oor
Q-score: 0.597
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Chromatin remodeller-nucleosome complex at 3.6 A resolution.
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6gej
Q-score: 0.372
Willhoft O, Ghoneim M, Lin CL, Chua EYD, Wilkinson M, Chaban Y, Ayala R, McCormack EA, Ocloo L, Rueda DS, Wigley DB
Science (2018) 362 [ DOI: doi:10.1126/science.aat7716 Pubmed: 30309918 ]
- Ruvb-like protein 1 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ruvb-like protein 2 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from synthetic construct)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar protein sorting-associated protein 72 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (154-mer) (47 kDa, DNA from synthetic construct)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Actin-like protein arp6 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (154-mer) (47 kDa, DNA from synthetic construct)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Histone (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar protein sorting-associated protein 71 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Swr1-nucleosome complex (1 MDa, Complex)
- Zinc ion (65 Da, Ligand)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Remodeller (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Helicase swr1 (174 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Chromatin remodeller-nucleosome complex at 4.5 A resolution.
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6gen
Q-score: 0.195
Willhoft O, Ghoneim M, Lin CL, Chua EYD, Wilkinson M, Chaban Y, Ayala R, McCormack EA, Ocloo L, Rueda DS, Wigley DB
Science (2018) 362 [ DOI: doi:10.1126/science.aat7716 Pubmed: 30309918 ]
- Ruvb-like protein 1 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ruvb-like protein 2 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from synthetic construct)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Vacuolar protein sorting-associated protein 72 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Actin-like protein arp6 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histones (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (173-mer) (53 kDa, DNA from synthetic construct)
- Vacuolar protein sorting-associated protein 71 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Swr1-nucleosome complex (1 MDa, Complex)
- Zinc ion (65 Da, Ligand)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Remodeller (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (173-mer) (53 kDa, DNA from synthetic construct)
- Helicase swr1 (174 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))