Cryo-EM structure of the whole photosynthetic complex from the green sulfur bacteria
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7z6q
Q-score: 0.493
Xie H, Lyratzakis A, Khera R, Koutantou M, Welsch S, Michel H, Tsiotis G
PNAS (2023) 120 pp. e2216734120-e2216734120 [ DOI: doi:10.1073/pnas.2216734120 Pubmed: 36693097 ]
- Bacteriochlorophyll a (911 Da, Ligand)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- [(2~{r})-2-hexadecanoyloxy-3-[(2~{s},3~{s},4~{r},5~{r},6~{s})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-propyl] hexadecanoate (731 Da, Ligand)
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Photosystem p840 reaction center (490 kDa, Complex from Chlorobaculum tepidum TLS)
- Chlorophyll a (893 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8gwa
Q-score: 0.541
Chen JH, Wang W, Wang C, Kuang T, Shen JR, Zhang X
J Integr Plant Biol (2023) 65 pp. 223-234 [ Pubmed: 36125941 DOI: doi:10.1111/jipb.13367 ]
- Chlorophyll a ester (891 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Gsbrc-2fmo complex (Complex from Chlorobaculum tepidum TLS)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Unkown protein (4 kDa, Protein from Chlorobaculum tepidum TLS)
- Ric1 protein (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- Iron/sulfur cluster (351 Da, Ligand)
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO1)
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7uea
Q-score: 0.406
Puskar R, Du Truong C, Swain K, Chowdhury S, Chan KY, Li S, Cheng KW, Wang TY, Poh YP, Mazor Y, Liu H, Chou TF, Nannenga BL, Chiu PL
Nat Commun (2022) 13 pp. 5824-5824 [ DOI: doi:10.1038/s41467-022-33505-4 Pubmed: 36192412 ]
- Chlorophyll a ester (891 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Photosynthetic assembly (rc-fmo1) (370 kDa, Complex from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO2)
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7ueb
Q-score: 0.497
Puskar R, Du Truong C, Swain K, Chowdhury S, Chan KY, Li S, Cheng KW, Wang TY, Poh YP, Mazor Y, Liu H, Chou TF, Nannenga BL, Chiu PL
Nat Commun (2022) 13 pp. 5824-5824 [ DOI: doi:10.1038/s41467-022-33505-4 Pubmed: 36192412 ]
- Chlorophyll a ester (891 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Psce (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Calcium ion (40 Da, Ligand)
- Pscf (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Photosynthetic assembly (rc-fmo2) (504 kDa, Complex from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
Cryo-EM structure of FMO-RC complex from green sulfur bacteria
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6m32
Q-score: 0.525
Chen JH, Wu H, Xu C, Liu XC, Huang Z, Chang S, Wang W, Han G, Kuang T, Shen JR, Zhang X
Science (2020) 370 [ Pubmed: 33214250 DOI: doi:10.1126/science.abb6350 ]
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a ester (891 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- Fmo-rc of green sulfur bacteria (Complex from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)