Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8gda
Q-score: 0.506
Huang SM, Xiong MY, Liu L, Mu J, Wang MW, Jia YL, Cai K, Tie L, Zhang C, Cao S, Wen X, Wang JL, Guo SC, Li Y, Qu CX, He QT, Cai BY, Xue C, Gan S, Xie Y, Cong X, Yang Z, Kong W, Li S, Li Z, Xiao P, Yang F, Yu X, Guan YF, Zhang X, Liu Z, Yang BX, Du Y, Sun JP
PNAS (2023) 120 pp. e2216329120-e2216329120 [ DOI: doi:10.1073/pnas.2216329120 Pubmed: 37478163 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Prostaglandin e receptor ep4 coupled to g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- (2s,5r)-5-[(1e,3s)-4,4-difluoro-3-hydroxy-4-phenylbut-1-en-1-yl]-1-[6-(1h-tetrazol-5-yl)hexyl]pyrrolidin-2-ol (421 Da, Ligand)
- Nb35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein subunit gamma (12 kDa, Protein from Homo sapiens)
- Prostaglandin e2 receptor ep4 subtype (53 kDa, Protein from Homo sapiens)
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8htu
Q-score: 0.55
Nat Plants (2023) 9 pp. 1347-1358 [ DOI: doi:10.1038/s41477-023-01463-4 Pubmed: 37474782 ]
- Phylloquinone (450 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit vi, chloroplastic (14 kDa, Protein from Physcomitrium patens)
- Photosystem i reaction center subunit iii (26 kDa, Protein from Physcomitrium patens)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Physcomitrium patens)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit viii (4 kDa, Protein from Physcomitrium patens)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Physcomitrium patens)
- Photosystem i iron-sulfur center (8 kDa, Protein from Physcomitrium patens)
- Photosystem i from physcomitrium patens (Complex from Physcomitrium patens)
- Chlorophyll a-b binding protein, chloroplastic (29 kDa, Protein from Physcomitrium patens)
- Chlorophyll b (907 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem i subunit x (13 kDa, Protein from Physcomitrium patens)
- Chlorophyll a-b binding protein, chloroplastic (33 kDa, Protein from Physcomitrium patens)
- Water (18 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (34 kDa, Protein from Physcomitrium patens)
- Photosystem i reaction center subunit v, chloroplastic (16 kDa, Protein from Physcomitrium patens)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Physcomitrium patens)
- Photosystem i reaction center subunit ii, chloroplastic (22 kDa, Protein from Physcomitrium patens)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Physcomitrium patens)
- Chlorophyll a-b binding protein, chloroplastic (28 kDa, Protein from Physcomitrium patens)
- Chlorophyll a-b binding protein, chloroplastic (28 kDa, Protein from Physcomitrium patens)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Physcomitrium patens)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Photosystem i subunit o (15 kDa, Protein from Physcomitrium patens)
- Photosystem i reaction center subunit iv, chloroplastic (13 kDa, Protein from Physcomitrium patens)
- Iron/sulfur cluster (351 Da, Ligand)
- Psi subunit v (23 kDa, Protein from Physcomitrium patens)
- Chlorophyll a (893 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)