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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"S4" AND xref_links:"pfam" AND author_name:"Yusupova G" AND author_name:"E"

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EMD-25100

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Unmethylated Mtb Ribosome 50S with SEQ-9

Release date: Oct. 12, 2022
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7sfr
Q-score: 0.468

Zhang J, Lair C, Roubert C, Amaning K, Barrio MB, Benedetti Y, Cui Z, Xing Z, Li X, Franzblau SG, Baurin N, Bordon-Pallier F, Cantalloube C, Sans S, Silve S, Blanc I, Fraisse L, Rak A, Jenner LB, Yusupova G, Yusupov M, Zhang J, Kaneko T, Yang TJ, Fotouhi N, Nuermberger E, Tyagi S, Betoudji F, Upton A, Sacchettini JC, Lagrange S

Cell (2023) 186 pp. 1013- [ Pubmed: 36827973 DOI: doi:10.1016/j.cell.2023.01.043 ]

Sample:

50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium tuberculosis)
16s rrna (498 kDa, RNA from Mycobacterium tuberculosis)
30s ribosomal protein s14 type z (6 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s17 (14 kDa, Protein from Mycobacterium tuberculosis)
Unmethylated mth ribosome with seq-9 (Complex from Mycobacterium tuberculosis)
50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s8 (14 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s20 (9 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s15 (10 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l19 (13 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l3 (22 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s3 (30 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s16 (17 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l5 (20 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s10 (11 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s18 (9 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l27 (8 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s4 (23 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s9 (16 kDa, Protein from Mycobacterium tuberculosis)
23s rrna (1 MDa, RNA from Mycobacterium tuberculosis)
50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium tuberculosis)
Peptide (2 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium tuberculosis)
Sequanamycin 9 (1 kDa, Ligand)
5s rrna (37 kDa, RNA from Mycobacterium tuberculosis)
30s ribosomal protein s13 (14 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s7 (17 kDa, Protein from Mycobacterium tuberculosis)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s19 (10 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l13 (21 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s6 (10 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium tuberculosis)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s12 (13 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s5 (22 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l9 (16 kDa, Protein from Mycobacterium tuberculosis)
30s ribosomal protein s11 (14 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l17 (19 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l4 (22 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l23 (10 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l22 (20 kDa, Protein from Mycobacterium tuberculosis)
50s ribosomal protein l33 (6 kDa, Protein from Mycobacterium tuberculosis)

[Show more]

EMD-22865

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CryoEM structure of A2296-methylated Mycobacterium tuberculosis ribosome bound with SEQ-9

Release date: Jan. 19, 2022
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7kgb
Q-score: 0.576

Zhang J, Lair C, Roubert C, Amaning K, Barrio MB, Benedetti Y, Cui Z, Xing Z, Li X, Franzblau SG, Baurin N, Bordon-Pallier F, Cantalloube C, Sans S, Silve S, Blanc I, Fraisse L, Rak A, Jenner LB, Yusupova G, Yusupov M, Zhang J, Kaneko T, Yang TJ, Fotouhi N, Nuermberger E, Tyagi S, Betoudji F, Upton A, Sacchettini JC, Lagrange S

Cell (2023) [ Pubmed: 36827973 DOI: doi:10.1016/j.cell.2023.01.043 ]

Sample:

30s ribosomal protein s17 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s14 type z (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s13 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l22 (20 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s9 (16 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s12 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
16s rrna (498 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l5 (21 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l19 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s8 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l37 (2 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s10 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s6 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
A2296-methylated mycobacterium tuberculosis ribosome bound with seq-9 (Complex from Mycobacterium tuberculosis H37Rv)
50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s16 (17 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l4 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Trna-initiator met(cau) (24 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
23s rrna (1 MDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l9 (16 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l13 (21 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s11 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s18 1 (9 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l3 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s19 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s4 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Sequanamycin 9 (1 kDa, Ligand)
30s ribosomal protein s15 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l33 2 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s3 (30 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s20 (9 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
5s rrna (37 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Zinc ion (65 Da, Ligand)
50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l17 (19 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l27 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s7 (17 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
30s ribosomal protein s5 (22 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
50s ribosomal protein l23 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))

[Show more]

EMD-0047

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Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP/sordarin)

Release date: July 11, 2018
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6gq1
Q-score: 0.357

Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y

J Mol Biol (2018) 430 pp. 2677-2687 [ DOI: doi:10.1016/j.jmb.2018.06.006 Pubmed: 29886014 ]

Sample:

60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
18s ribosomal rna (578 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
80s ribosome in complex with mrna, trna and eef2 in presence of non-hydrolizable gtp (gmppcp) and anti-fungal drug (sordarin) (3 MDa, Complex)
40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s15 (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
Ubiquitin-40s ribosomal protein s31 (4 kDa, Protein from Baker's yeast)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
60s ribosomal protein l14-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
5s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Messenger rna (2 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s5 (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s17-b (14 kDa, Protein from Baker's yeast)
5.8s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
40s ribosomal protein s18-a (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s26-b (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s3 (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
60s acidic ribosomal protein p0 (20 kDa, Protein from Baker's yeast)
40s ribosomal protein s6-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
60s ribosomal protein l42-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l43-a (9 kDa, Protein from Baker's yeast)
60s ribosomal protein l41-b (3 kDa, Protein from Baker's yeast)
40s ribosomal protein s1-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s23-a (15 kDa, Protein from Baker's yeast)
80s ribosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l34-a (12 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae s288c 35s pre-ribosomal rna (rdn37-1), miscrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l8-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l7-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-a (19 kDa, Protein from Baker's yeast)
60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
Rna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
40s ribosomal protein s9-a (21 kDa, Protein from Baker's yeast)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
60s ribosomal protein l12-a (10 kDa, Protein from Baker's yeast)
40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
Elongation factor 2 (93 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (10 kDa, Protein from Baker's yeast)
60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
40s ribosomal protein s30-a (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
Transfer rna - phe (24 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l11-b (19 kDa, Protein from Baker's yeast)
40s ribosomal protein s0-a (22 kDa, Protein from Baker's yeast)
40s ribosomal protein s12 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l25 (13 kDa, Protein from Baker's yeast)
[1r-(1.alpha.,3a.beta.,4.beta.,4a.beta.,7.beta.,7a.alpha.,8a.beta.)]8a-[(6-deoxy-4-o-methyl-beta-d-altropyranosyloxy)methyl]-4-formyl-4,4a,5,6,7,7a,8,8a-octahydro-7-methyl-3-(1-methylethyl)-1,4-methano-s-indacene-3a(1h)-carboxylic acid (494 Da, Ligand)
40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)
40s ribosomal protein s20 (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s14-b (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l24-a (7 kDa, Protein from Baker's yeast)
40s ribosomal protein s25-a (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s13 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s2 (23 kDa, Protein from Baker's yeast)
60s ribosomal protein l13-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)

[Show more]

EMD-0048

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Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GDP+AlF4/sordarin)

Release date: July 11, 2018
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6gqb
Q-score: 0.386

Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y

J Mol Biol (2018) 430 pp. 2677-2687 [ DOI: doi:10.1016/j.jmb.2018.06.006 Pubmed: 29886014 ]

Sample:

60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (11 kDa, Protein from Baker's yeast)
Elongation factor 2 (93 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
Mrna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ubiquitin-40s ribosomal protein s31 (4 kDa, Protein from Baker's yeast)
[1r-(1.alpha.,3a.beta.,4.beta.,4a.beta.,7.beta.,7a.alpha.,8a.beta.)]8a-[(6-deoxy-4-o-methyl-beta-d-altropyranosyloxy)methyl]-4-formyl-4,4a,5,6,7,7a,8,8a-octahydro-7-methyl-3-(1-methylethyl)-1,4-methano-s-indacene-3a(1h)-carboxylic acid (494 Da, Ligand)
60s ribosomal protein l14-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-a (19 kDa, Protein from Baker's yeast)
40s ribosomal protein s15 (14 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
40s ribosomal protein s5 (22 kDa, Protein from Baker's yeast)
Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Baker's yeast)
80s ribosome in complex with mrna, trna and eef2 in presence of gdp, alf4 and anti-fungal drug (sordarin) (3 MDa, Complex)
60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
Tetrafluoroaluminate ion (102 Da, Ligand)
40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
Messenger rna (2 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s18-a (16 kDa, Protein from Baker's yeast)
60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s17-b (14 kDa, Protein from Baker's yeast)
18s ribosomal rna (578 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l42-a (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
60s acidic ribosomal protein p0 (20 kDa, Protein from Baker's yeast)
40s ribosomal protein s6-a (25 kDa, Protein from Baker's yeast)
40s ribosomal protein s9-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l43-a (9 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l41-b (3 kDa, Protein from Baker's yeast)
40s ribosomal protein s1-a (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (10 kDa, Protein from Baker's yeast)
60s ribosomal protein l11-b (19 kDa, Protein from Baker's yeast)
40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
Saccharomyces cerevisiae s288c 35s pre-ribosomal rna (rdn37-1), miscrna (1 MDa, RNA from Baker's yeast)
60s ribosomal protein l24-a (7 kDa, Protein from Baker's yeast)
Guanosine-5'-diphosphate (443 Da, Ligand)
40s ribosomal protein s23-a (15 kDa, Protein from Baker's yeast)
80s ribosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l34-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l8-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l1-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s3 (24 kDa, Protein from Baker's yeast)
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s26-b (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s30-a (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
Transfer rna - phe (24 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s0-a (22 kDa, Protein from Baker's yeast)
5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l12-a (10 kDa, Protein from Baker's yeast)
40s ribosomal protein s12 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l25 (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l7-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)
40s ribosomal protein s20 (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s2 (23 kDa, Protein from Baker's yeast)
40s ribosomal protein s14-b (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s25-a (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s13 (16 kDa, Protein from Baker's yeast)
60s ribosomal protein l13-a (21 kDa, Protein from Baker's yeast)
5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)

[Show more]

EMD-0049

Download Map 3D View

Cryo-EM recosntruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP)

Release date: July 11, 2018
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6gqv
Q-score: 0.378

Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y

J Mol Biol (2018) 430 pp. 2677-2687 [ DOI: doi:10.1016/j.jmb.2018.06.006 Pubmed: 29886014 ]

Sample:

60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
40s ribosomal protein s3 (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s23-a (15 kDa, Protein from Baker's yeast)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
60s ribosomal protein l11-b (19 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
18s ribosomal rna (578 kDa, RNA from Saccharomyces cerevisiae)
Ubiquitin-40s ribosomal protein s31 (4 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
60s ribosomal protein l13-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l42-a (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
60s ribosomal protein l41-b (3 kDa, Protein from Baker's yeast)
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s15 (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
40s ribosomal protein s26-b (11 kDa, Protein from Baker's yeast)
40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l14-a (14 kDa, Protein from Baker's yeast)
60s acidic ribosomal protein p0 (20 kDa, Protein from Baker's yeast)
40s ribosomal protein s13 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s1-a (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s9-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s2 (23 kDa, Protein from Baker's yeast)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
Elongation factor 2 (92 kDa, Protein from Baker's yeast)
5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
60s ribosomal protein l7-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
Mrna (Complex from Saccharomyces cerevisiae)
40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (10 kDa, Protein from Baker's yeast)
40s ribosomal protein s6-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l12-a (10 kDa, Protein from Baker's yeast)
60s ribosomal protein l8-a (25 kDa, Protein from Baker's yeast)
40s ribosomal protein s30-a (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
40s ribosomal protein s14-b (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l25 (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s18-a (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s17-b (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s5 (22 kDa, Protein from Baker's yeast)
40s ribosomal protein s0-a (22 kDa, Protein from Baker's yeast)
40s ribosomal protein s12 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l1-a (22 kDa, Protein from Baker's yeast)
Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
80s ribosome (Complex from Saccharomyces cerevisiae)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s25-a (8 kDa, Protein from Baker's yeast)
Transfer rna - phe (24 kDa, RNA from Saccharomyces cerevisiae)
60s ribosomal protein l43-a (9 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-a (19 kDa, Protein from Baker's yeast)
80s ribosome in complex with mrna, trna and eef2 in presence of non-hydrolyzable gtp (gmppcp) (Complex)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l34-a (12 kDa, Protein from Baker's yeast)
Messenger rna (2 kDa, RNA from Saccharomyces cerevisiae)
40s ribosomal protein s20 (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l24-a (7 kDa, Protein from Baker's yeast)
60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)

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