Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the presence of telomeric ssDNA
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8sok
Q-score: 0.283
Cai SW, Takai H, Zaug AJ, Dilgen TC, Cech TR, Walz T, de Lange T
Cell (2024) 187 pp. 3638-3651.e18 [ Pubmed: 38838667 DOI: doi:10.1016/j.cell.2024.05.002 ]
- Ssdna-bound human cst-pot1(esdl)/tpp1 complex (370 kDa, Complex from Homo sapiens)
- Cst complex subunit stn1 (42 kDa, Protein from Homo sapiens)
- Protection of telomeres protein 1 (72 kDa, Protein from Homo sapiens)
- Tpp1 (58 kDa, Protein from Homo sapiens)
- Dna (5'-d(*tp*tp*ap*gp*gp*gp*tp*tp*ap*g)-3') (5 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Cst complex subunit ctc1 (178 kDa, Protein from Homo sapiens)
- Cst complex subunit ten1 (13 kDa, Protein from Homo sapiens)
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 8d0k
Q-score: 0.311
He Q, Lin X, Chavez BL, Agrawal S, Lusk BL, Lim CJ
Nature (2022) 608 pp. 826-832 [ Pubmed: 35830881 DOI: doi:10.1038/s41586-022-05040-1 ]
- Dna polymerase alpha subunit b (67 kDa, Protein from Homo sapiens)
- Dna primase large subunit (60 kDa, Protein from Homo sapiens)
- Human cst-dna polymerase alpha/primase advanced preinitiation complex bound to 4xtel-foldback dna template (540 kDa, Complex from Homo sapiens)
- Dna (5'-d(p*tp*ap*gp*gp*gp*tp*tp*ap*gp*gp*gp*tp*tp*ap*gp*gp*gp*tp*tp*a)-3') (18 kDa, DNA from Homo sapiens)
- Dna primase small subunit (51 kDa, Protein from Homo sapiens)
- Dna polymerase alpha catalytic subunit (172 kDa, Protein from Homo sapiens)
- Cst complex subunit ctc1 (138 kDa, Protein from Homo sapiens)
- Cst complex subunit stn1 (43 kDa, Protein from Homo sapiens)
- Cst complex subunit ten1 (17 kDa, Protein from Homo sapiens)
Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7u5c
Q-score: 0.33
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Dna polymerase alpha subunit b (66 kDa, Protein from Homo sapiens)
- Cst complex subunit stn1 (42 kDa, Protein from Homo sapiens)
- Dna primase small subunit (49 kDa, Protein from Homo sapiens)
- Cst complex subunit ctc1 (135 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (59 kDa, Protein from Homo sapiens)
- Canonical telomeric dna sequence (5 kDa, DNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cst complex subunit ten1 (13 kDa, Protein from Homo sapiens)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Homo sapiens)
- Human cst bound to dna polymerase alpha(pola1 delta n)-primase in a recruitment state (500 kDa, Complex)