Cryo-EM structure of Escherichia coli AcrBZ and DARPin in Saposin A-nanodisc with cardiolipin
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 6sgr
Q-score: 0.54
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump accessory protein acrz (Complex from Shigella boydii 965-58)
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrbz and darpin in saposin a-nanodisc with cardiolipin (Complex)
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Escherichia coli K-12)
- Darpin (Complex from synthetic construct)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K-12)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrBZ and DARPin in Saposin A-nanodisc
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6sgs
Q-score: 0.425
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump accessory protein acrz (Complex from Shigella boydii 965-58)
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Shigella boydii 965-58)
- Ternary complex of acrbz and darpin in saposin a-nanodisc (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc with cardiolipin
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 6sgt
Q-score: 0.483
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrb and darpin in saposina-nanodisc with cardiolipin (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 6sgu
Q-score: 0.477
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure (2020) 28 pp. 625- [ DOI: doi:10.1016/j.str.2020.03.013 Pubmed: 32348749 ]
- Multidrug efflux pump subunit acrb (Complex from Escherichia coli K-12)
- Ternary complex of acrb and darpin in saposina-nanodisc (Complex)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli K12)
- Darpin (Complex from synthetic construct)
- Darpin (18 kDa, Protein from synthetic construct)
Structure of AtTPC1(DDE) reconstituted in saposin A with cat06 Fab
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6e1k
Q-score: 0.408
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
PNAS (2018) 115 pp. E9095-E9104 [ DOI: doi:10.1073/pnas.1805651115 Pubmed: 30190435 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Cat06 light chain (23 kDa, Protein from Homo sapiens)
- Cat06 heavy chain (24 kDa, Protein from Homo sapiens)
- Attpc1(dde) reconstituted in saposin a (Complex from Arabidopsis thaliana)
- Calcium ion (40 Da, Ligand)