WT Chicken Scap L1-L7 / Fab 4G10 complex focused refinement
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lkf
Q-score: 0.586
Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM
Cell (2021) 184 pp. 3689- [ DOI: doi:10.1016/j.cell.2021.05.019 Pubmed: 34139175 ]
- 4g10 light chain (23 kDa, Protein from Mus musculus)
- 4g10 fab, 4g10 light chain (Complex from Mus musculus)
- Scap l1-l7 domain (50 kDa, Complex from Gallus gallus)
- Sterol regulatory element-binding protein cleavage-activating protein (148 kDa, Protein from Gallus gallus)
- Ternary complex of scap with fab fragment (172 kDa, Complex)
- 4g10 heavy chain (24 kDa, Protein from Mus musculus)
Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM
Cell (2021) 184 pp. 3689-3701.e22 [ DOI: doi:10.1016/j.cell.2021.05.019 Pubmed: 34139175 ]
- Sterol regulatory element-binding protein cleavage-activating protein (Protein from Gallus gallus)
- 4g10 fab light chain (Protein from Mouse)
- Ternary complex of scap with fab fragment (50 kDa, Complex)
- 4g10 fab (Complex from Mus musculus)
- 4g10 fab heavy chain (Protein from Mouse)
- Scap (Complex from Gallus gallus)
Map of full mutant cScap/Fab complex
Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM
Cell (2021) 184 pp. 3689-3701.e22 [ DOI: doi:10.1016/j.cell.2021.05.019 Pubmed: 34139175 ]
- Insig-1 (Protein from Gallus gallus)
- Sterol regulatory element-binding protein cleavage-activating protein (Protein from Gallus gallus)
- 4g10 fab light chain (Protein from Mouse)
- Ternary complex of scap with fab fragment (50 kDa, Complex)
- 4g10 fab (Complex from Mus musculus)
- 4g10 fab heavy chain (Protein from Mouse)
- Scap (Complex from Gallus gallus)
Chicken Scap D435V L1-L7 domain / Fab complex focused map
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7lkh
Q-score: 0.497
Kober DL, Radhakrishnan A, Goldstein JL, Brown MS, Clark LD, Bai XC, Rosenbaum DM
Cell (2021) 184 pp. 3689- [ DOI: doi:10.1016/j.cell.2021.05.019 Pubmed: 34139175 ]
- 4g10 fab kappa chain (23 kDa, Protein from Mus musculus)
- Scap l1-l7 domain (50 kDa, Complex from Gallus gallus)
- 4g10 fab heavy chain (24 kDa, Protein from Mus musculus)
- Ternary complex of scap with fab fragment (172 kDa, Complex)
- 4g10 fab (Complex from Mus musculus)
- Sterol regulatory element-binding protein cleavage-activating protein (148 kDa, Protein from Gallus gallus)
Cryo-EM structure of Scap/Insig complex in the present of digitonin.
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7etw
Q-score: 0.351
Yan R, Cao P, Song W, Li Y, Wang T, Qian H, Yan C, Yan N
Cell Rep (2021) 35 pp. 109299- [ DOI: doi:10.1016/j.celrep.2021.109299 ]
- Sterol regulatory element-binding protein cleavage-activating protein (81 kDa, Protein from Homo sapiens)
- Scap and insig complex (Complex from Homo sapiens)
- Digitonin (1 kDa, Ligand)
- Insulin-induced gene 2 protein (24 kDa, Protein from Homo sapiens)
cryo-EM structure of Scap/Insig complex in the present of 25-hydroxyl cholesterol.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6m49
Q-score: 0.335
Yan R, Cao P, Song W, Qian H, Du X, Coates HW, Zhao X, Li Y, Gao S, Gong X, Liu X, Sui J, Lei J, Yang H, Brown AJ, Zhou Q, Yan C, Yan N
Science (2021) 371 [ Pubmed: 33446483 DOI: doi:10.1126/science.abb2224 ]
- 25-hydroxycholesterol (402 Da, Ligand)
- Scap and insig complex (Complex from Homo sapiens)
- Sterol regulatory element-binding protein cleavage-activating protein,sterol regulatory element-binding protein cleavage-activating protein (63 kDa, Protein from Homo sapiens)
- Insulin-induced gene 2 protein (24 kDa, Protein from Homo sapiens)
Complex structure of the fission yeast SREBP-SCAP binding domains
Resolution: 5.4 Å
EM Method: Single-particle
Fitted PDBs: 5grs
Q-score: 0.202
Gong X, Qian HW, Shao W, Li J, Wu JP, Liu JJ, Li W, Wang HW, Espenshade P, Yan N
Cell Res (2016) 26 pp. 1197-1211 [ Pubmed: 27811944 DOI: doi:10.1038/cr.2016.123 ]
- Complex structure of the fission yeast srebp-scap binding domains (Complex from Schizosaccharomyces pombe 972h-)
- Sterol regulatory element-binding protein cleavage-activating protein (44 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Sterol regulatory element-binding protein 1 (30 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Sterol regulatory element-binding protein cleavage-activating protein (11 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))