Unmethylated Mtb Ribosome 50S with SEQ-9
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7sfr
Q-score: 0.468
Zhang J, Lair C, Roubert C, Amaning K, Barrio MB, Benedetti Y, Cui Z, Xing Z, Li X, Franzblau SG, Baurin N, Bordon-Pallier F, Cantalloube C, Sans S, Silve S, Blanc I, Fraisse L, Rak A, Jenner LB, Yusupova G, Yusupov M, Zhang J, Kaneko T, Yang TJ, Fotouhi N, Nuermberger E, Tyagi S, Betoudji F, Upton A, Sacchettini JC, Lagrange S
Cell (2023) 186 pp. 1013- [ DOI: doi:10.1016/j.cell.2023.01.043 Pubmed: 36827973 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s16 (17 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l19 (13 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l4 (22 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l5 (20 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l3 (22 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s17 (14 kDa, Protein from Mycobacterium tuberculosis)
- Peptide (2 kDa, Protein from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s5 (22 kDa, Protein from Mycobacterium tuberculosis)
- Unmethylated mth ribosome with seq-9 (Complex from Mycobacterium tuberculosis)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l13 (21 kDa, Protein from Mycobacterium tuberculosis)
- Sequanamycin 9 (1 kDa, Ligand)
- 16s rrna (498 kDa, RNA from Mycobacterium tuberculosis)
- 50s ribosomal protein l17 (19 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s11 (14 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s18 (9 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s19 (10 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s20 (9 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s4 (23 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l9 (16 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s15 (10 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s9 (16 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s3 (30 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l33 (6 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l27 (8 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s12 (13 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium tuberculosis)
- 23s rrna (1 MDa, RNA from Mycobacterium tuberculosis)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s7 (17 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s8 (14 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l23 (10 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l22 (20 kDa, Protein from Mycobacterium tuberculosis)
- 30s ribosomal protein s6 (10 kDa, Protein from Mycobacterium tuberculosis)
- 50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium tuberculosis)
- 5s rrna (37 kDa, RNA from Mycobacterium tuberculosis)
- 30s ribosomal protein s13 (14 kDa, Protein from Mycobacterium tuberculosis)
CryoEM structure of A2296-methylated Mycobacterium tuberculosis ribosome bound with SEQ-9
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7kgb
Q-score: 0.576
Zhang J, Lair C, Roubert C, Amaning K, Barrio MB, Benedetti Y, Cui Z, Xing Z, Li X, Franzblau SG, Baurin N, Bordon-Pallier F, Cantalloube C, Sans S, Silve S, Blanc I, Fraisse L, Rak A, Jenner LB, Yusupova G, Yusupov M, Zhang J, Kaneko T, Yang TJ, Fotouhi N, Nuermberger E, Tyagi S, Betoudji F, Upton A, Sacchettini JC, Lagrange S
Cell (2023) [ Pubmed: 36827973 DOI: doi:10.1016/j.cell.2023.01.043 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s13 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l33 2 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s11 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l5 (21 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l3 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s6 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l9 (16 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l22 (20 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s3 (30 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l37 (2 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s10 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s7 (17 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s9 (16 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Sequanamycin 9 (1 kDa, Ligand)
- 50s ribosomal protein l13 (21 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s4 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s8 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- A2296-methylated mycobacterium tuberculosis ribosome bound with seq-9 (Complex from Mycobacterium tuberculosis H37Rv)
- 50s ribosomal protein l19 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- Trna-initiator met(cau) (24 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s20 (9 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s14 type z (6 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s16 (17 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s19 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s18 1 (9 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 23s rrna (1 MDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l23 (10 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l27 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 5s rrna (37 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 16s rrna (498 kDa, RNA from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s12 (13 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l4 (23 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l17 (19 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s5 (22 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 30s ribosomal protein s17 (14 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
- 50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))