Cryo-EM structure of the tetrameric SPARSA gRNA-ssDNA-NAD+ complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8y82
Q-score: 0.412
Cui N, Zhang JT, Li Z, Wei XY, Wang J, Jia N
Nat Commun (2024) 15 pp. 8610-8610 [ Pubmed: 39366953 DOI: doi:10.1038/s41467-024-52910-5 ]
- Rna (21-mer) (6 kDa, RNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Tetrameric sparsagrna-ssdna-nad+ complex (Complex from Geobacter sulfurreducens)
- Magnesium ion (24 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
The Cryo-EM structure of DSR2-Tail tube-NAD+ complex
Wang R, Xu Q, Wu Z, Li J, Guo H, Liao T, Shi Y, Yuan L, Gao H, Yang R, Shi Z, Li F
Nat Commun (2024) 15 pp. 6185-6185 [ DOI: doi:10.1038/s41467-024-50410-0 Pubmed: 39039073 ]
- Tail tube protein (29 kDa, Protein from Bacillus subtilis)
- Sir2-like domain-containing protein (118 kDa, Protein from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Dsr2-tail tube protein-nad+ complex (Complex from Bacillus subtilis)
The structure of DSR2 and NAD+ complex
Int J Biol Macromol (2024) 277 pp. 134145-134145 [ Pubmed: 39059542 DOI: doi:10.1016/j.ijbiomac.2024.134145 ]
Cryo-EM structure of DSR2 (H171A)-tube-NAD+ complex
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 8wyb
Q-score: 0.334
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Bacillus phage spr tube protein (29 kDa, Protein from Bacillus phage SPR)
- Sir2-like domain-containing protein (118 kDa, Protein from Bacillus subtilis)
- Cryoem structure of dsr2 (h171a)-tube-nad+ complex (Complex from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Cryo-EM structure of DSR2 (H171A)-tube-NAD+ (partial) complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8wyc
Q-score: 0.547
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Cryoem structure of dsr2 (h171a)-tube nad+ (half) complex (Complex from Bacillus subtilis)
- Bacillus phage spr tube protein (29 kDa, Protein from Bacillus phage SPR)
- Sir2-like domain-containing protein (118 kDa, Protein from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
Cryo-EM structure of DSR2-DSAD1-NAD+ (partial) complex
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8wyf
Q-score: 0.546
Zhang JT, Liu XY, Li Z, Wei XY, Song XY, Cui N, Zhong J, Li H, Jia N
Nat Commun (2024) 15 pp. 2797-2797 [ DOI: doi:10.1038/s41467-024-47177-9 Pubmed: 38555355 ]
- Sir2 family protein (118 kDa, Protein from Bacillus subtilis)
- Cryoem structure of dsr2 -dsad1-nad+ (half) complex (Complex from Bacillus subtilis)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Bacillus phage spbeta dsad1 protein (16 kDa, Protein from Bacillus phage SPBc2)
Cryo-EM structure of the prokaryotic SPARSA system complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8jkz
Q-score: 0.475
Zhen X, Xu X, Ye L, Xie S, Huang Z, Yang S, Wang Y, Li J, Long F, Ouyang S
Nat Commun (2024) 15 pp. 450-450 [ Pubmed: 38200015 DOI: doi:10.1038/s41467-023-44660-7 ]
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- Sir2/ago (Complex from Geobacter sulfurreducens PCA)
- Ago (Complex)
- Sir2 (Complex from Geobacter sulfurreducens PCA)
Cryo-EM structure of the prokaryotic SPARSA system complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8jl0
Q-score: 0.547
Zhen X, Xu X, Ye L, Xie S, Huang Z, Yang S, Wang Y, Li J, Long F, Ouyang S
Nat Commun (2024) 15 pp. 450-450 [ Pubmed: 38200015 DOI: doi:10.1038/s41467-023-44660-7 ]
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Piwi domain protein (53 kDa, Protein from Geobacter sulfurreducens)
- The sir2/ago/guide rna/recognition dna complex (Complex from Geobacter sulfurreducens PCA)
- Dna/guide rna duplex (Complex)
- Rna (5'-r(p*ap*up*ap*ap*up*gp*gp*up*up*up*cp*up*up*ap*gp*ap*cp*gp*up*cp*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Ago (Complex)
- Dna (5'-d(p*ap*cp*gp*ap*cp*gp*tp*cp*tp*ap*ap*gp*ap*ap*ap*cp*cp*ap*tp*tp*ap*t)-3') (6 kDa, DNA from synthetic construct)
- Sir2 (Complex from Geobacter sulfurreducens PCA)
- Sir2 superfamily protein (66 kDa, Protein from Geobacter sulfurreducens)