Overall map of a transcribing RNA polymerase II-U1 snRNP complex with 145-nt RNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7b0y
Q-score: 0.344
Zhang S, Aibara S, Vos SM, Agafonov DE, Luhrmann R, Cramer P
Science (2021) 371 pp. 305-309 [ DOI: doi:10.1126/science.abf1870 Pubmed: 33446560 ]
- U1 snrnp (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa domesticus)
- 145-nt rna (46 kDa, RNA from synthetic construct)
- Rna polymerase ii (Complex from Sus scrofa domesticus)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Rpb12 (7 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa domesticus)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Nucleic acids (Complex from synthetic construct)
- Template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa domesticus)
- U1 small nuclear ribonucleoprotein a (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit d (16 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa domesticus)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit f (14 kDa, Protein from Sus scrofa domesticus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from Sus scrofa domesticus)
- U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- The transcribing rna polymerase ii-u1 snrnp complex with 145-nt rna (Complex)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa domesticus)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Sus scrofa domesticus)
- U1 snrna (52 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein-associated protein (23 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa domesticus)
- Non-template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa domesticus)
Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6j6h
Q-score: 0.283
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ Pubmed: 30879786 DOI: doi:10.1016/j.cell.2019.02.006 ]
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Catalytically activated spliceosome b*-a1 complex (Complex from Saccharomyces cerevisiae S288C)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Act1 pre-mrna (217 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Skip (62 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Ring finger protein 113a (38 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Homo sapiens)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Early bact spliceosome (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- Bud13 homolog (70 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5zwm
Q-score: 0.162
Bai R, Wan R, Yan C, Lei J, Shi Y
Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]
- U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
- U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- 66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
- U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-bps (13 kDa, RNA from Saccharomyces cerevisiae S288c)
- U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
- Yeast fully assembled spliceosomal pre-b complex before activation (Cell from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
- Zinc ion (65 Da, Ligand)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6g90
Q-score: 0.152
Plaschka C, Lin PC, Charenton C, Nagai K
Nature (2018) 559 pp. 419-422 [ Pubmed: 29995849 DOI: doi:10.1038/s41586-018-0323-8 ]
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp11,pre-mrna-splicing factor prp11,pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae)
- Yeast ubc4 pre-mrna (mutant) (12 kDa, RNA from Saccharomyces cerevisiae)
- U2 snrna (45 kDa, RNA from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
- Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae)
- U1 snrna,u1 snrna,u1 snrna,u1 snrna,u1 snrna (126 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae)
- U1 small nuclear ribonucleoprotein component prp42 (65 kDa, Protein from Saccharomyces cerevisiae)
- U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-processing factor 39 (74 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae)
- Protein luc7,protein luc7,protein luc7 (28 kDa, Protein from Saccharomyces cerevisiae)
- U1 small nuclear ribonucleoprotein c (27 kDa, Protein from Saccharomyces cerevisiae)
- U1 small nuclear ribonucleoprotein 70 kda homolog,u1 small nuclear ribonucleoprotein 70 kda homolog,u1 small nuclear ribonucleoprotein 70 kda homolog (33 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Prespliceosome a complex (1 MDa, Complex from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
- Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- U1 small nuclear ribonucleoprotein component snu71 (71 kDa, Protein from Saccharomyces cerevisiae)
- Unknown (4 kDa, Protein from Saccharomyces cerevisiae)
- 56 kda u1 small nuclear ribonucleoprotein component (56 kDa, Protein from Saccharomyces cerevisiae)
- Protein nam8 (57 kDa, Protein from Saccharomyces cerevisiae)
- U1 small nuclear ribonucleoprotein a,u1 small nuclear ribonucleoprotein a,u1 small nuclear ribonucleoprotein a,u1 small nuclear ribonucleoprotein a,u1 small nuclear ribonucleoprotein a (33 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5xjc
Q-score: 0.22
Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y
Cell (2017) 169 pp. 918-929.e14 [ DOI: doi:10.1016/j.cell.2017.04.033 Pubmed: 28502770 ]
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Protein casc3 (76 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Prkr-interacting protein 1 (21 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (100 kDa, Protein from Homo sapiens)
- Homo sapiens small nuclear rna (u2) gene (60 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Homo sapiens)
- Protein mago nashi homolog 2 (17 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Homo sapiens)
- Rna-binding protein 8a (19 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
- Pre-mrna (87 kDa, RNA from Human adenovirus 2)
- Human c* spliceosome (Complex from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
Structure of a pre-catalytic spliceosome (B2 map)
Resolution: 7.2 Å
EM Method: Single-particle
Fitted PDBs: 5nrl
Q-score: 0.117
Nature (2017) 546 pp. 617-621 [ Pubmed: 28530653 DOI: doi:10.1038/nature22799 ]
- Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor spp381 (33 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Unknown (7 kDa, Protein from Saccharomyces cerevisiae)
- Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae )
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Yeast ubc4 gene for ubiquitin-conjugating enzyme (30 kDa, RNA from Saccharomyces cerevisiae)
- 23 kda u4/u6.u5 small nuclear ribonucleoprotein component (22 kDa, Protein from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae)
- U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae )
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
- 66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae)
- Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae)
- Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor 38 (27 kDa, Protein from Saccharomyces cerevisiae)
- Pre-catalytic b complex spliceosome (2 MDa, Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae )
- U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae)
- U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae)
- U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae)
- Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 5mqf
Q-score: 0.202
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lu Hrmann R
Nature (2017) 542 pp. 318-323 [ Pubmed: 28076346 DOI: doi:10.1038/nature21079 ]
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Human gene for small nuclear rna u2 (snrna u2) (60 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Human c* spliceosome, masked refinement (Complex from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Homo sapiens rna, u6 small nuclear 1 (rnu6-1), small nuclear rna (34 kDa, RNA from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Minx pre-mrna (intron) (104 kDa, RNA from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Intron-binding protein aquarius (173 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Homo sapiens u5 a small nuclear rna (36 kDa, RNA from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
Structure of the yeast spliceosome immediately after branching. 3D class containing helicase module.
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 5lj5
Q-score: 0.114
Galej WP, Wilkinson ME, Fica SM, Oubridge C, Newman AJ, Nagai K
Nature (2016) 537 pp. 197-201 [ DOI: doi:10.1038/nature19316 Pubmed: 27459055 ]
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae)
- Spliceosome immediately after branching (2 MDa, Complex from Saccharomyces cerevisiae)
- U2 snrna (small nuclear rna) (376 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae)
- Unknown (16 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Saccharomyces cerevisiae)
- Exon 1 (5' exon) of ubc4 pre-mrna (5 kDa, RNA from Saccharomyces cerevisiae)
- U5 snrna (small nuclear rna) (57 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae)
- Intron of ubc4 pre-mrna (24 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae)
- Protein cwc16 (32 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- U6 snrna (small nuclear rna) (35 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae)
- Cwc22 (69 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Cwc15 (19 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5gmk
Q-score: 0.279
Wan R, Yan C, Bai R, Huang G, Shi Y
Science (2016) 353 pp. 895-904 [ DOI: doi:10.1126/science.aag2235 Pubmed: 27445308 ]
- Pre-mrna-splicing factor syf1 (78 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor cef1 (61 kDa, Protein from Saccharomyces cerevisiae S288C)
- Intron_bps (9 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288C)
- Spliceosomal c complex (Complex from Saccharomyces cerevisiae S288c)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae S288C)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae S288C)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5'-exon (4 kDa, RNA from Saccharomyces cerevisiae S288c)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor clf1 (68 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5'-splicing site (4 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- Protein cwc16 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae S288C)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288C)