Structure of the CNOT3-bound human 80S ribosome with tRNA-ARG in the P-site.
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 9c3h
Q-score: 0.684
Zhu X, Cruz VE, Zhang H, Erzberger JP, Mendell JT
Science (2024) 386 pp. eadq8587-eadq8587 [ DOI: doi:10.1126/science.adq8587 Pubmed: 39571015 ]
- Large ribosomal subunit protein ul24 (17 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us19 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el18 (21 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein es10 (18 kDa, Protein from Homo sapiens)
- Mrna (2 kDa, RNA from Homo sapiens)
- Large ribosomal subunit protein el40 (11 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us7 (22 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el43 (10 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us2 (32 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el6 (32 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es19 (16 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el15 (24 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 80s ribosome in complex with cnot3 and p-site trna arg ccg-1 (4 MDa, Complex from Homo sapiens)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Homo sapiens)
- Receptor of activated protein c kinase 1 (35 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el13 (24 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es26 (13 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul30 (29 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein el27 (15 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein el28 (15 kDa, Protein from Homo sapiens)
- Ccr4-not transcription complex subunit 3 (64 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el29 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el36 (12 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us9 (16 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul6 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul13 (23 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el34 (13 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul14 (14 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us13 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el20 (20 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us11 (16 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul18 (34 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us12 (15 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es1 (30 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es6 (28 kDa, Protein from Homo sapiens)
- 18s rrna (603 kDa, RNA from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Nascent chain (3 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es25 (13 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el21 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul2 (28 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul3 (46 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us17 (18 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el32 (15 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el37 (11 kDa, Protein from Homo sapiens)
- Fau ubiquitin-like and ribosomal protein s30 (14 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es12 (14 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul4 (47 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us4 (22 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el33 (12 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es4, x isoform (29 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es32 (3 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul29 (14 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein us5 (31 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es21 (9 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es7 (22 kDa, Protein from Homo sapiens)
- Trna-arg,ccg-1 (24 kDa, RNA from Homo sapiens)
- Small ribosomal subunit protein us15 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el8 (30 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el30 (12 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul16 (24 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es8 (24 kDa, Protein from Homo sapiens)
- Isoform 3 of small ribosomal subunit protein es24 (15 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el14 (23 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es27 (9 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el22 (14 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es28 (7 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- Large ribosomal subunit protein el24 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul23 (17 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Homo sapiens)
- Small ribosomal subunit protein es17 (15 kDa, Protein from Homo sapiens)
- 2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol (282 Da, Ligand)
- Large ribosomal subunit protein el38 (8 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el19 (23 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el31 (14 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein el42 (12 kDa, Protein from Homo sapiens)