E. coli 70S-TEC complex in delivery state
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 9gr1
Q-score: 0.421
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli BL21(DE3))
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 70s ribosome (Complex from Escherichia coli BL21(DE3))
- Zinc ion (65 Da, Ligand)
- Mrna in ribosome channel (5 kDa, RNA from synthetic construct)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Phenylalanine (165 Da, Ligand)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BL21(DE3))
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs6, fully modified isoform (15 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli BL21(DE3))
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BL21(DE3))
- Mrna in sd-asd duplex (3 kDa, RNA from synthetic construct)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us7 (20 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 23s ribosomal rna (948 kDa, RNA from Escherichia coli BL21(DE3))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli BL21(DE3))
30S mRNA delivery complex (open head)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9gup
Q-score: 0.516
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
30S PIC (Pre-Initiation complex)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9guq
Q-score: 0.487
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6, non-modified isoform (15 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
30S mRNA delivery complex TEC resolved (30S only)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9gus
Q-score: 0.401
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
30S mRNA delivery complex (bS1 resolved)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9gut
Q-score: 0.536
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (999 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
30S mRNA delivery complex (consensus)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9guu
Q-score: 0.549
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
30S mRNA delivery complex (closed-head)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9guv
Q-score: 0.561
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosome (Complex from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
30S-TEC (TEC in expressome position) Inactive state 2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9guw
Q-score: 0.394
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ Pubmed: 39607923 DOI: doi:10.1126/science.ado8476 ]
- Tec (Complex from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Template dna strand (11 kDa, DNA from synthetic construct)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit bound to tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Non-template dna strand (12 kDa, DNA from synthetic construct)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
30S-TEC (TEC in expressome position) Inactive state 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9gux
Q-score: 0.374
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- Template dna strand (11 kDa, DNA from synthetic construct)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal subunit boud to tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- Tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- Non-template dna strand (11 kDa, DNA from synthetic construct)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)