Human TRiC complex in closed state with nanobody bound (Consensus Map)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7nvl
Q-score: 0.631
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ DOI: doi:10.1038/s41594-022-00755-1 Pubmed: 35449234 ]
- Water (18 Da, Ligand)
- Nanobody nb18 (14 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nanobody nb18 (Complex from Lama glama)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Human tric complex with nanobody bound (Complex)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
Human TRiC complex in closed state with nanobody Nb18, actin and PhLP2A bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7nvm
Q-score: 0.529
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ DOI: doi:10.1038/s41594-022-00755-1 Pubmed: 35449234 ]
- Human type ii chaperonin tric/cct (Complex from Homo sapiens)
- T-complex protein (Complex from Homo sapiens)
- Nanobody nb18 (14 kDa, Protein from Lama glama)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- Nanobody (Complex from Lama glama)
- Actin, cytoplasmic 2 (41 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human type ii chaperonin tric/cct complex with nanobody nb18, actin and phlp2a bound (Complex)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
Human TRiC complex in closed state with nanobody and tubulin bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7nvn
Q-score: 0.558
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ DOI: doi:10.1038/s41594-022-00755-1 Pubmed: 35449234 ]
- Nanobody (14 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit epsilon (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Human type ii chaperonin tric/cct complex with nanobody nb18 and tubulin bound (Complex)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Nanobody (Complex from Lama glama)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Tubulin beta-2a chain (49 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
Human TRiC complex in open state with nanobody bound
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7nvo
Q-score: 0.502
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ DOI: doi:10.1038/s41594-022-00755-1 Pubmed: 35449234 ]
- Human type ii chaperonin tric/cct complex with nanobody bound (Complex)
- Nanobody (14 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Water (18 Da, Ligand)
- Nanobody nb18 (Complex from Lama glama)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
Human TRiC complex in open state with nanobody bound (map-only)
Kelly JJ, Chi G, Bulawa C, Paavilainen VO, Bountra C, Huiskonen JT, Yue W
To Be Published
- T-complex 1 subunit beta (Protein from Homo sapiens)
- T-complex 1 subunit alpha (Protein from Homo sapiens)
- T-complex protein 1 subunit theta (Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex with nanobody bound (Complex from Homo sapiens)
- T-complex protein 1 subunit eta (Protein from Homo sapiens)
- T-complex 1 subunit delta (Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (Protein from Homo sapiens)