Human TRiC-PhLP2A complex in the closed state
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8i1u
Q-score: 0.506
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ Pubmed: 38307855 DOI: doi:10.1038/s41467-024-45242-x ]
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- Complex of tric/cct-phlp2a treated with atp-alfx (1 MDa, Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Phlp2a (pdcl3) (Complex)
The focused refinement of CCT3-PhLP2A from TRiC-PhLP2A complex in the open state
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8i6j
Q-score: 0.404
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ Pubmed: 38307855 DOI: doi:10.1038/s41467-024-45242-x ]
- Tric/cct (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phlp2a (pdcl3) (Complex from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- Complex of tric/cct and phlp2a (1 MDa, Complex from Homo sapiens)
Human TRiC-PhLP2A complex in the open state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8i9u
Q-score: 0.286
Park J, Kim H, Gestaut D, Lim S, Opoku-Nsiah KA, Leitner A, Frydman J, Roh SH
Nat Commun (2024) 15 pp. 1007-1007 [ Pubmed: 38307855 DOI: doi:10.1038/s41467-024-45242-x ]
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Phlp2a (pdcl3) in human tric/cct complex (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Complex of human tric/cct and phlp2a (1 MDa, Complex)
Prefoldin-tubulin-TRiC complex
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 7wu7
Q-score: 0.302
Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J
Cell (2022) 185 pp. 4770-4787.e20 [ Pubmed: 36493755 DOI: doi:10.1016/j.cell.2022.11.014 ]
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Prefoldin subunit 3 (22 kDa, Protein from Homo sapiens)
- Prefoldin subunit 1 (14 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Prefoldin subunit 2 (16 kDa, Protein from Homo sapiens)
- Prefoldin subunit 5 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- Prefoldin subunit 6 (14 kDa, Protein from Homo sapiens)
- Prefoldin subunit 4 (16 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Ternary complex of tric/cct, beta-tubulin, prefoldin complex (1 MDa, Complex from Homo sapiens)
Human TRiC complex in closed state with nanobody bound (Consensus Map)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7nvl
Q-score: 0.631
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ Pubmed: 35449234 DOI: doi:10.1038/s41594-022-00755-1 ]
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Nanobody nb18 (Complex from Lama glama)
- Nanobody nb18 (14 kDa, Protein from Lama glama)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- Human tric complex with nanobody bound (Complex)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
Human TRiC complex in closed state with nanobody Nb18, actin and PhLP2A bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7nvm
Q-score: 0.529
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ Pubmed: 35449234 DOI: doi:10.1038/s41594-022-00755-1 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nanobody (Complex from Lama glama)
- Nanobody nb18 (14 kDa, Protein from Lama glama)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct (Complex from Homo sapiens)
- T-complex protein (Complex from Homo sapiens)
- Actin, cytoplasmic 2 (41 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Phosducin-like protein 3 (27 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Human type ii chaperonin tric/cct complex with nanobody nb18, actin and phlp2a bound (Complex)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
Human TRiC complex in closed state with nanobody and tubulin bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7nvn
Q-score: 0.558
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ Pubmed: 35449234 DOI: doi:10.1038/s41594-022-00755-1 ]
- Nanobody (14 kDa, Protein from Lama glama)
- Tubulin beta-2a chain (49 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human type ii chaperonin tric/cct complex with nanobody nb18 and tubulin bound (Complex)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- Nanobody (Complex from Lama glama)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (Complex from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
Human TRiC complex in open state with nanobody bound
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7nvo
Q-score: 0.502
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
Nat Struct Mol Biol (2022) 29 pp. 420-429 [ Pubmed: 35449234 DOI: doi:10.1038/s41594-022-00755-1 ]
- Nanobody (14 kDa, Protein from Lama glama)
- Magnesium ion (24 Da, Ligand)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Nanobody nb18 (Complex from Lama glama)
- Aluminum fluoride (83 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex (Complex from Homo sapiens)
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- Human type ii chaperonin tric/cct complex with nanobody bound (Complex)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)
Human TRiC/CCT complex with reovirus outer capsid protein sigma-3
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 7lup
Q-score: 0.217
Knowlton JJ, Gestaut D, Ma B, Taylor G, Seven AB, Leitner A, Wilson GJ, Shanker S, Yates NA, Prasad BVV, Aebersold R, Chiu W, Frydman J, Dermody TS
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2018127118 Pubmed: 33836586 ]
- Water (18 Da, Ligand)
- T-complex protein 1 subunit beta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit eta (59 kDa, Protein from Homo sapiens)
- Human tric/cct complex with reovirus outer capsid protein sigma-3 (41 MDa, Complex)
- T-complex protein 1 subunit gamma (60 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit alpha (60 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- T-complex protein 1 subunit theta (59 kDa, Protein from Homo sapiens)
- Reovirus outer capsid protein sigma-3 (Complex from Mammalian orthoreovirus 3 Dearing)
- Human tric/cct complex (Complex from Homo sapiens)
- Outer capsid protein sigma-3 (41 kDa, Protein from Reovirus type 3 (strain Dearing))
- T-complex protein 1 subunit delta (57 kDa, Protein from Homo sapiens)
- T-complex protein 1 subunit zeta (58 kDa, Protein from Homo sapiens)