Cryo-EM structure of the Cas12m2-crRNA-target DNA full R-loop complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8hhl
Q-score: 0.547
Omura SN, Nakagawa R, Sudfeld C, Villegas Warren R, Wu WY, Hirano H, Laffeber C, Kusakizako T, Kise Y, Lebbink JHG, Itoh Y, van der Oost J, Nureki O
Nat Struct Mol Biol (2023) 30 pp. 1172-1182 [ Pubmed: 37460897 DOI: doi:10.1038/s41594-023-01042-3 ]
- Cas12m2 (Complex)
- Crrna (Complex)
- Target dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Cas12m2-crrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Ts (36-mer) (10 kDa, DNA from Mycolicibacterium mucogenicum)
- Cas12m2 (66 kDa, Protein from Mycolicibacterium mucogenicum)
- Crrna-target dna (Complex)
- Zinc ion (65 Da, Ligand)
- Crrna (56-mer) (18 kDa, RNA from Mycolicibacterium mucogenicum)
- Nts (36-mer) (11 kDa, DNA from Mycolicibacterium mucogenicum)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and DNA
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7lys
Q-score: 0.517
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ Pubmed: 34381246 DOI: doi:10.1038/s41594-021-00632-3 ]
- Cryo-em map of casphi bound to crrna and dna (124 kDa, Complex from Biggievirus Mos11)
- Crrna (14 kDa, RNA from Phage #D)
- Casphi-2 (86 kDa, Protein from Biggievirus Mos11)
- Ts-dna (12 kDa, DNA from Phage #D)
- Nts-dna (11 kDa, DNA from Phage #D)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and Phosphorothioate-DNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lyt
Q-score: 0.524
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ Pubmed: 34381246 DOI: doi:10.1038/s41594-021-00632-3 ]
- Magnesium ion (24 Da, Ligand)
- Ts-dna (13 kDa, DNA from Biggievirus Mos11)
- Crrna (14 kDa, RNA from Biggievirus Mos11)
- Nts-dna* (13 kDa, DNA from Biggievirus Mos11)
- Zinc ion (65 Da, Ligand)
- Cryo-em map of casphi bound to crrna and phosphorothioate-dna in the presence of the nuclease magnesium cofactor (128 kDa, Complex from Biggievirus Mos11)
- Casphi (86 kDa, Protein from Biggievirus Mos11)
Cryo-EM structure of Cas12i(E894A)-crRNA-dsDNA complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6w5c
Q-score: 0.526
Zhang H, Li Z, Xiao R, Chang L
Nat Struct Mol Biol (2020) 27 pp. 1069-1076 [ Pubmed: 32895556 DOI: doi:10.1038/s41594-020-0499-0 ]
- Ts (11 kDa, DNA from Lachnospiraceae bacterium ND2006)
- Substrate (2 kDa, DNA from Lachnospiraceae bacterium ND2006)
- Nts (2 kDa, DNA from Lachnospiraceae bacterium ND2006)
- Crispr-cas complex (Complex from Lachnospiraceae bacterium ND2006)
- Crrna (18 kDa, RNA from Escherichia coli)
- Cas12i (125 kDa, Protein from Lachnospiraceae bacterium ND2006)