Cryo-EM structure of CasLambda (Cas12l) bound to crRNA and DNA
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8dc2
Q-score: 0.491
Al-Shayeb B, Skopintsev P, Soczek KM, Stahl EC, Li Z, Groover E, Smock D, Eggers AR, Pausch P, Cress BF, Huang CJ, Staskawicz B, Savage DF, Jacobsen SE, Banfield JF, Doudna JA
Cell (2022) 185 pp. 4574-4586.e16 [ Pubmed: 36423580 DOI: doi:10.1016/j.cell.2022.10.020 ]
- Dna ts (14 kDa, DNA from uncultured virus)
- Caslambda-crrna-dsdna ternary structure (Complex from uncultured virus)
- Dna nts (14 kDa, DNA from uncultured virus)
- Rna (51-mer) (16 kDa, RNA from uncultured virus)
- Caslambda (87 kDa, Protein from uncultured virus)
BG505.MD39TS Env trimer in complex with Fab from antibody C05
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7sq1
Q-score: 0.406
Xu Z, Walker S, Wise MC, Chokkalingam N, Purwar M, Moore A, Tello-Ruiz E, Wu Y, Majumdar S, Konrath KM, Kulkarni A, Tursi NJ, Zaidi FI, Reuschel EL, Patel I, Obeirne A, Du J, Schultheis K, Gites L, Smith T, Mendoza J, Broderick KE, Humeau L, Pallesen J, Weiner DB, Kulp DW
Nat Commun (2022) 13 pp. 695-695 [ DOI: doi:10.1038/s41467-022-28363-z Pubmed: 35121758 ]
- C05 fab light chain (12 kDa, Protein from Mus musculus)
- Envelope glycoprotein gp160 (52 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- C05 fab heavy chain (13 kDa, Protein from Mus musculus)
- Transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Bg505.md39ts env trimer in complex with fab from antibody c05 (410 kDa, Complex)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and DNA
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7lys
Q-score: 0.517
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ Pubmed: 34381246 DOI: doi:10.1038/s41594-021-00632-3 ]
- Crrna (14 kDa, RNA from Phage #D)
- Ts-dna (12 kDa, DNA from Phage #D)
- Nts-dna (11 kDa, DNA from Phage #D)
- Casphi-2 (86 kDa, Protein from Biggievirus Mos11)
- Cryo-em map of casphi bound to crrna and dna (124 kDa, Complex from Biggievirus Mos11)
Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and Phosphorothioate-DNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7lyt
Q-score: 0.524
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA
Nat Struct Mol Biol (2021) 28 pp. 652-661 [ Pubmed: 34381246 DOI: doi:10.1038/s41594-021-00632-3 ]
- Zinc ion (65 Da, Ligand)
- Nts-dna* (13 kDa, DNA from Biggievirus Mos11)
- Ts-dna (13 kDa, DNA from Biggievirus Mos11)
- Cryo-em map of casphi bound to crrna and phosphorothioate-dna in the presence of the nuclease magnesium cofactor (128 kDa, Complex from Biggievirus Mos11)
- Crrna (14 kDa, RNA from Biggievirus Mos11)
- Magnesium ion (24 Da, Ligand)
- Casphi (86 kDa, Protein from Biggievirus Mos11)