CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 9ar6
Q-score: 0.501
Roth M, Shu Y, Trasanidou D, Zhao Y, van der Oost J, Li H
To Be Published
- Target-strand dna (12 kDa, DNA from Geobacillus thermodenitrificans)
- Sodium ion (22 Da, Ligand)
- Ternary complex of thermocas9 with single guide rna and target dna (Complex from Geobacillus thermodenitrificans)
- Non-target dna (3 kDa, DNA from Geobacillus thermodenitrificans)
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
- Water (18 Da, Ligand)
Cascade complex from type I-A CRISPR-Cas system
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7tr9
Q-score: 0.225
Hu C, Ni D, Nam KH, Majumdar S, McLean J, Stahlberg H, Terns MP, Ke A
Mol Cell (2022) 82 pp. 2754-2768.e5 [ Pubmed: 35835111 DOI: doi:10.1016/j.molcel.2022.06.007 ]
- Nickel (ii) ion (58 Da, Ligand)
- Cas5a (29 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Target strand dna (6 kDa, DNA from Escherichia coli)
- Non-target strand dna (2 kDa, DNA from Escherichia coli)
- Cas11a (12 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Crispr-associated helicase cas3 (61 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Crrna (45-mer) (14 kDa, RNA from Escherichia coli)
- Crispr-associated endonuclease cas3-hd (26 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Cas8a (38 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Cas7a (36 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Partial r-loop state of cas3-cascade complex from pyrococcus furiosus type i-a crispr/cas system (450 kDa, Complex from Geobacter sulfurreducens)
Cascade complex from type I-A CRISPR-Cas system
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7tra
Q-score: 0.324
Hu C, Ni D, Nam KH, Majumdar S, McLean J, Stahlberg H, Terns MP, Ke A
Mol Cell (2022) 82 pp. 2754-2768.e5 [ Pubmed: 35835111 DOI: doi:10.1016/j.molcel.2022.06.007 ]
- Full r-loop state of cas3-cascade complex from pyrococcus furiosus type i-a crispr/cas system (450 kDa, Complex from Geobacter sulfurreducens)
- Nickel (ii) ion (58 Da, Ligand)
- Cas5a (29 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Cas11a (12 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Crispr-associated helicase cas3 (61 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Crrna (44-mer) (14 kDa, RNA from Escherichia coli)
- Cas8a (38 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Crispr-associated endonuclease cas3-hd (24 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Cas7a (36 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Non-target strand dna (25-mer) (7 kDa, DNA from Escherichia coli)
- Target strand dna (44-mer) (13 kDa, DNA from Escherichia coli)