NTP-bound Pol IV transcription elongation complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8xmb
Q-score: 0.43
Fang C, Huang K, Wu X, Zhang H, Gu Z, Wang J, Zhang Y
Sci Adv (2024) 10 pp. eadq3087-eadq3087 [ DOI: doi:10.1126/sciadv.adq3087 Pubmed: 39178250 ]
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Rna (4 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iv subunit 7 (19 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Template_dna (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Ntp-bound pol iv transcription elongation complex (650 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dna-directed rna polymerase iv subunit 1 (168 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 4 (22 kDa, Protein from Arabidopsis thaliana)
- Nontemplate_dna (6 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
Post-translocated Pol IV transcription elongation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8xmc
Q-score: 0.417
Fang C, Huang K, Wu X, Zhang H, Gu Z, Wang J, Zhang Y
Sci Adv (2024) 10 pp. eadq3087-eadq3087 [ DOI: doi:10.1126/sciadv.adq3087 Pubmed: 39178250 ]
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Post-translocated pol iv transcription elongation complex (650 kDa, Complex from Arabidopsis thaliana)
- Rna (4 kDa, RNA from synthetic construct)
- Template dna (9 kDa, DNA from synthetic construct)
- Non-template dna (6 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iv subunit 7 (19 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (168 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 4 (22 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
Pre-translocated Pol IV transcription elongation complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8xmd
Q-score: 0.383
Fang C, Huang K, Wu X, Zhang H, Gu Z, Wang J, Zhang Y
Sci Adv (2024) 10 pp. eadq3087-eadq3087 [ DOI: doi:10.1126/sciadv.adq3087 Pubmed: 39178250 ]
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Template_dna (10 kDa, DNA from synthetic construct)
- Pre-translocated pol iv transcription elongation complex (650 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 7 (19 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (168 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 4 (22 kDa, Protein from Arabidopsis thaliana)
- Nontemplate_dna (6 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna (5 kDa, RNA from synthetic construct)
Backtracked Pol IV transcription elongation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8xme
Q-score: 0.377
Fang C, Huang K, Wu X, Zhang H, Gu Z, Wang J, Zhang Y
Sci Adv (2024) 10 pp. eadq3087-eadq3087 [ DOI: doi:10.1126/sciadv.adq3087 Pubmed: 39178250 ]
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Template_dna (12 kDa, DNA from synthetic construct)
- Backtracked pol iv transcription elongation complex (650 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 7 (19 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (168 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 4 (22 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Nontemplate_dna (12 kDa, DNA from synthetic construct)
- Rna (5 kDa, RNA from synthetic construct)