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sample_name:"Transcription elongation factor Spt4" AND author_name:"Sekine S"
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Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9j0n
Q-score: 0.316

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0o
Q-score: 0.313

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0p
Q-score: 0.311

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

Release date: Nov. 29, 2023
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 8jh2
Q-score: 0.145

Akatsu M, Ehara H, Kujirai T, Fujita R, Ito T, Osumi K, Ogasawara M, Takizawa Y, Sekine SI, Kurumizaka H

J Biol Chem (2023) 299 pp. 105477-105477 [ Pubmed: 37981206 DOI: doi:10.1016/j.jbc.2023.105477 ]

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RNA polymerase II elongation complex transcribing a nucleosome (EC49B)

Release date: Oct. 12, 2022
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7xt7
Q-score: 0.207

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ Pubmed: 35981082 DOI: doi:10.1126/science.abp9466 ]

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RNA polymerase II elongation complex transcribing a nucleosome (EC58hex)

Release date: Oct. 12, 2022
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7xti
Q-score: 0.223

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

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RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C

Release date: Sept. 7, 2022
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xn7
Q-score: 0.338

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

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RNA polymerase II elongation complex transcribing a nucleosome (EC42)

Release date: Sept. 7, 2022
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7xse
Q-score: 0.238

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

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RNA polymerase II elongation complex transcribing a nucleosome (EC49)

Release date: Sept. 7, 2022
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7xsx
Q-score: 0.227

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

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RNA polymerase II elongation complex transcribing a nucleosome (EC115)

Release date: Sept. 7, 2022
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7xsz
Q-score: 0.248

Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI

Science (2022) 377 pp. eabp9466-eabp9466 [ DOI: doi:10.1126/science.abp9466 Pubmed: 35981082 ]

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