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sample_name:"Transcription elongation factor Spt4" AND database:"EMDB" AND resolution:[3 TO 4}
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Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9j0n
Q-score: 0.316

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0o
Q-score: 0.313

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0p
Q-score: 0.311

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 30 bp upstream

Release date: Dec. 18, 2024
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9eh0
Q-score: 0.24

Markert JW, Soffers JH, Farnung L

Science (2025) 387 pp. 528-533 [ DOI: doi:10.1126/science.adn6319 Pubmed: 39666822 ]

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RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream

Release date: Dec. 18, 2024
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9eh1
Q-score: 0.237

Markert JW, Soffers JH, Farnung L

Science (2025) 387 pp. 528-533 [ DOI: doi:10.1126/science.adn6319 Pubmed: 39666822 ]

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RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-FACT nucleosome upstream

Release date: Dec. 18, 2024
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9eh2
Q-score: 0.252

Markert JW, Soffers JH, Farnung L

Science (2025) 387 pp. 528-533 [ DOI: doi:10.1126/science.adn6319 Pubmed: 39666822 ]

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Structure of mammalian Pol II-DSIF-SPT6-PAF1-TFIIS-hexasome elongation complex

Release date: July 31, 2024
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a3y
Q-score: 0.231

Farnung L, Ochmann M, Garg G, Vos SM, Cramer P

Mol Cell (2022) 82 pp. 3126-3134.e7 [ Pubmed: 35858621 DOI: doi:10.1016/j.molcel.2022.06.027 ]

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Cryo-EM structure of Pyrococcus furiosus transcription elongation complex bound to Spt4/5

Release date: April 24, 2024
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8oki
Q-score: 0.48

Tarau D, Grunberger F, Pilsl M, Reichelt R, Heiss F, Konig S, Urlaub H, Hausner W, Engel C, Grohmann D

Nucleic Acids Res (2024) 52 pp. 6017-6035 [ Pubmed: 38709902 DOI: doi:10.1093/nar/gkae282 ]

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Cryo-EM structure of Pyrococcus furiosus apo form RNA polymerase contracted clamp conformation with Spt4/5

Release date: April 24, 2024
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8p2i
Q-score: 0.461

Tarau D, Grunberger F, Pilsl M, Reichelt R, Heiss F, Konig S, Urlaub H, Hausner W, Engel C, Grohmann D

Nucleic Acids Res (2024) 52 pp. 6017-6035 [ Pubmed: 38709902 DOI: doi:10.1093/nar/gkae282 ]

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human co-transcriptional RNA capping enzyme RNGTT

Release date: April 10, 2024
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8w8e
Q-score: 0.265

Li Y, Wang Q, Xu Y, Li Z

Nat Commun (2024) 15 pp. 4622-4622 [ Pubmed: 38816438 DOI: doi:10.1038/s41467-024-48963-1 ]

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