CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (the consensus NusA-EC)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7py3
Q-score: 0.365
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Tdna (11 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Cryoem structure of e.coli rna polymerase elongation complex bound to nusa (the consensus nusa-ec) (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli MS 115-1)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7py5
Q-score: 0.367
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Nusa and nusg elongation complex (consensus) (Complex from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7py6
Q-score: 0.348
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Tdna (11 kDa, DNA from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Nusa and nusg elongation complex in non-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7py7
Q-score: 0.329
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Nusa and nusg elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli MS 115-1)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7pyj
Q-score: 0.276
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Tdna (11 kDa, DNA from Escherichia coli)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Nusa elongation complex in non-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7pyk
Q-score: 0.295
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Ntdna (12 kDa, DNA from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Nusa elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
CryoEM structure of E.coli RNA polymerase paused elongation complex bound to NusA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6flq
Q-score: -0.011
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol Cell (2018) 69 pp. 816-827.e4 [ Pubmed: 29499136 DOI: doi:10.1016/j.molcel.2018.02.008 ]
- Nucleic acids (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*cp*cp*up*gp*ap*up*cp*ap*gp*gp*cp*gp*ap*up*gp*up*gp*up*gp*cp*u)-3') (6 kDa, RNA from synthetic construct)
- Cryo-em structure of e. coli rna polymerase paused elongation complex bound to nusa (Complex)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase, nusa (Complex from Escherichia coli K-12)
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)