Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8dy7
Q-score: 0.482
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ Pubmed: 36888660 DOI: doi:10.1073/pnas.2220785120 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae)
- Dna (71-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Streptomyces venezuelae rnap transcription open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Dna (78-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Probable cell division protein whia (37 kDa, Protein from Streptomyces venezuelae)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8dy9
Q-score: 0.492
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ Pubmed: 36888660 DOI: doi:10.1073/pnas.2220785120 ]
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Probable cell division protein whia (34 kDa, Protein from Streptomyces venezuelae)
- Streptomyces venezuelae rnap unconstrained open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae ATCC 10712)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 7kif
Q-score: 0.49
Lilic M, Darst SA, Campbell EA
Mol Cell (2021) 81 pp. 2875-2886.e5 [ Pubmed: 34171296 DOI: doi:10.1016/j.molcel.2021.05.017 ]
- Rna polymerase-binding transcription factor card (17 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna (63-mer) (30 kDa, DNA from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Probable transcriptional regulator whib7 (10 kDa, Protein from Mycobacterium tuberculosis)
- Dna (55-mer) (30 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis wt rnap transcription open promoter complex with whib7 transcription factor (Complex from Mycobacterium tuberculosis)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis)
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7kim
Q-score: 0.409
Lilic M, Darst SA, Campbell EA
Mol Cell (2021) 81 pp. 2875-2886.e5 [ Pubmed: 34171296 DOI: doi:10.1016/j.molcel.2021.05.017 ]
- Dna (45-mer) (30 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase-binding transcription factor card (17 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Probable transcriptional regulator whib7 (10 kDa, Protein from Mycobacterium tuberculosis)
- Dna (45-mer) (30 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis wt rnap transcription closed promoter complex with whib7 transcription factor (Complex from Mycobacterium tuberculosis)