Cryo-EM density map of Tn4430 TnpA hyperactive mutant (TnpA3X) in complex with IR48 substrate.
Fernandez M, Shkumatov AV, Liu Y, Stulemeijer C, Derclaye S, Efremov RG, Hallet B, Alsteens D
Nucleic Acids Res (2023) 51 pp. 4929-4941 [ DOI: doi:10.1093/nar/gkad241 Pubmed: 37026471 ]
- Ir48 substrate (Complex from Bacillus thuringiensis)
- Ir48 dna substrate, non transferred strand (DNA from Bacillus thuringiensis)
- Tnpa 3x mutant (Complex from Bacillus thuringiensis)
- Transposase for transposon tn4430 (Protein from Bacillus thuringiensis)
- Ir48 transferred strand (DNA from Bacillus thuringiensis)
- Tnpa3x-ir48 complex (Complex)
Medium resolution cryo-EM density map of Tn4430 TnpA transposase from Tn3 family in apo state
Fernandez M, Shkumatov AV, Liu Y, Stulemeijer C, Derclaye S, Efremov RG, Hallet B, Alsteens D
Nucleic Acids Res (2023) 51 pp. 4929-4941 [ DOI: doi:10.1093/nar/gkad241 Pubmed: 37026471 ]
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7qd4
Q-score: 0.446
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ Pubmed: 36257990 DOI: doi:10.1038/s41467-022-33871-z ]
- Ir100 dna substrate, transferred strand (30 kDa, DNA from Bacillus thuringiensis)
- Dna substrate (Complex from Bacillus thuringiensis)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Ir100 dna substrate, none transferred strand (30 kDa, DNA from Bacillus thuringiensis)
- Tnpa-ir100 complex (Complex)
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qd5
Q-score: 0.462
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ Pubmed: 36257990 DOI: doi:10.1038/s41467-022-33871-z ]
- Dna substrate (Complex from Bacillus thuringiensis)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Tnpa-ir48 complex (Complex)
- Ir48 transferred strand (14 kDa, DNA from Bacillus thuringiensis)
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
- Ir48 dna substrate, non transferred strand (14 kDa, DNA from Bacillus thuringiensis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7qd6
Q-score: 0.351
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ Pubmed: 36257990 DOI: doi:10.1038/s41467-022-33871-z ]
- Tnpa-ir71st complex (Complex)
- Dna substrate (Complex from Bacillus thuringiensis)
- Ir71st transferred strand (24 kDa, DNA from Bacillus thuringiensis)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Ir71st transferred strand (7 kDa, DNA from Bacillus thuringiensis)
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
- Ir71st non transferred strand (21 kDa, DNA from Bacillus thuringiensis)
Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in apo state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7qd8
Q-score: 0.352
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ Pubmed: 36257990 DOI: doi:10.1038/s41467-022-33871-z ]