Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7tdd
Q-score: 0.293
Dickinson MS, Lu J, Gupta M, Marten I, Hedrich R, Stroud RM
PNAS (2022) 119 [ Pubmed: 35210362 DOI: doi:10.1073/pnas.2110936119 ]
- Two pore calcium channel protein 1 (78 kDa, Protein from Arabidopsis thaliana)
- Attpc1 d454n-edta state ii (168 kDa, Complex from Arabidopsis thaliana)
AtTPC1 DDE mutant with 1 mM Ca2+
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7tde
Q-score: 0.358
Dickinson MS, Lu J, Gupta M, Marten I, Hedrich R, Stroud RM
PNAS (2022) 119 [ Pubmed: 35210362 DOI: doi:10.1073/pnas.2110936119 ]
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Attpc1 dde with 1 mm ca2+ (168 kDa, Complex from Arabidopsis thaliana)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7tbg
Q-score: 0.547
Dickinson MS, Lu J, Gupta M, Marten I, Hedrich R, Stroud RM
PNAS (2022) 119 [ Pubmed: 35210362 DOI: doi:10.1073/pnas.2110936119 ]
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Water (18 Da, Ligand)
- Two-pore channel 1 dimer (168 kDa, Complex from Arabidopsis thaliana)
- Sodium ion (22 Da, Ligand)
- Calcium ion (40 Da, Ligand)
AtTPC1 D454N with 1 mM EDTA state I
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7tdf
Q-score: 0.522
Dickinson MS, Lu J, Gupta M, Marten I, Hedrich R, Stroud RM
PNAS (2022) 119 [ Pubmed: 35210362 DOI: doi:10.1073/pnas.2110936119 ]
- Attpc1 d454n with 1 mm edta state i (168 kDa, Complex from Arabidopsis thaliana)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
Structure of AtTPC1(DDE) reconstituted in saposin A with cat06 Fab
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6e1k
Q-score: 0.408
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
PNAS (2018) 115 pp. E9095-E9104 [ DOI: doi:10.1073/pnas.1805651115 Pubmed: 30190435 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Cat06 light chain (23 kDa, Protein from Homo sapiens)
- Cat06 heavy chain (24 kDa, Protein from Homo sapiens)
- Attpc1(dde) reconstituted in saposin a (Complex from Arabidopsis thaliana)
- Calcium ion (40 Da, Ligand)
Structure of AtTPC1(DDE) reconstituted in saposin A
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6e1m
Q-score: 0.462
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
PNAS (2018) 115 pp. E9095-E9104 [ DOI: doi:10.1073/pnas.1805651115 Pubmed: 30190435 ]
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Attpc1(dde) (Complex from Arabidopsis thaliana)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of AtTPC1(DDE) in state 1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6e1n
Q-score: 0.398
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
PNAS (2018) 115 pp. E9095-E9104 [ DOI: doi:10.1073/pnas.1805651115 Pubmed: 30190435 ]
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Attpc1(dde) (Complex from Arabidopsis thaliana)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of AtTPC1(DDE) in state 2
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6e1p
Q-score: 0.355
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
PNAS (2018) 115 pp. E9095-E9104 [ DOI: doi:10.1073/pnas.1805651115 Pubmed: 30190435 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Attpc1(dde) (Complex from Arabidopsis thaliana)
- Two pore calcium channel protein 1 (84 kDa, Protein from Arabidopsis thaliana)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of mouse TPC1 channel in the apo state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6c96
Q-score: 0.456
She J, Guo J, Chen Q, Zeng W, Jiang Y, Bai XC
Nature (2018) 556 pp. 130-134 [ Pubmed: 29562233 DOI: doi:10.1038/nature26139 ]
- Sodium ion (22 Da, Ligand)
- Apo mouse tpc1 (Cellular component from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Two pore calcium channel protein 1 (95 kDa, Protein from Mus musculus)
Cryo-EM structure of mouse TPC1 channel in the PtdIns(3,5)P2-bound state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6c9a
Q-score: 0.49
She J, Guo J, Chen Q, Zeng W, Jiang Y, Bai XC
Nature (2018) 556 pp. 130-134 [ Pubmed: 29562233 DOI: doi:10.1038/nature26139 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-3-{[(s)-hydroxy{[(1s,2r,3r,4s,5s,6r)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate (746 Da, Ligand)
- Two pore calcium channel protein 1 (95 kDa, Protein from Mus musculus)
- Ptdins(3,5)p2-bound mouse tpc1 (Cellular component from Mus musculus)