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Tissue biology and disease modelling

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Epigenetics and neurobiology

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sample_name:"U6 snRNA-associated Sm-like protein LSm3" AND software_ft:"RELION"

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EMD-11695

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Human pre-Bact-1 spliceosome

Release date: Dec. 23, 2020
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 7abg
Q-score: 0.147

Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R

Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]

Sample:

Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
U2 snrna (60 kDa, RNA from Homo sapiens)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
U6 snrna (34 kDa, RNA from Homo sapiens)
Serine/arginine-rich splicing factor 1 (27 kDa, Protein from Homo sapiens)
Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
Guanosine-5'-triphosphate (523 Da, Ligand)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
U5 snrna (36 kDa, RNA from Homo sapiens)
116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
Pre-bact-1 spliceosomal complex (Complex)
Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
Nuclear cap-binding protein subunit 1 (91 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
Ww domain-binding protein 11 (70 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)

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EMD-4658

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Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.

Release date: April 17, 2019
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 6qw6
Q-score: 0.361

Charenton C, Wilkinson ME, Nagai K

Science (2019) 364 pp. 362-367 [ DOI: doi:10.1126/science.aax3289 Pubmed: 30975767 ]

Sample:

U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
Pre-mrna-processing-splicing factor 8 (271 kDa, Protein from Homo sapiens)
116 kda u5 small nuclear ribonucleoprotein component (95 kDa, Protein from Homo sapiens)
U4/u6.u5 tri-snrnp-associated protein 2 (64 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
Human u4/u6.u5 tri-snrnp (1 MDa, Complex)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
U5.u4/u6 tri-snrnp (Complex from Homo sapiens)
Probable atp-dependent rna helicase ddx23 (Complex from Homo sapiens)
U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
Guanosine-5'-triphosphate (523 Da, Ligand)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
U5 snrna (37 kDa, RNA from Homo sapiens)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
Nhp2-like protein 1 (14 kDa, Protein from Homo sapiens)
U6 snrna (28 kDa, RNA from Homo sapiens)
U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
Inositol hexakisphosphate (660 Da, Ligand)
U4 snrna (46 kDa, RNA from Homo sapiens)
Zinc ion (65 Da, Ligand)
Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)

[Show more]

EMD-4665

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Structure of a human fully-assembled precatalytic spliceosome (pre-B complex).

Release date: April 17, 2019
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 6qx9
Q-score: 0.207

Charenton C, Wilkinson ME, Nagai K

Science (2019) 364 pp. 362-367 [ DOI: doi:10.1126/science.aax3289 Pubmed: 30975767 ]

Sample:

Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
Pre-mrna-processing-splicing factor 8 (271 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
U4/u6.u5 tri-snrnp-associated protein 2 (64 kDa, Protein from Homo sapiens)
Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
U1 small nuclear ribonucleoprotein c (17 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
Splicing factor 3a subunit 1,splicing factor 3a subunit 1,splicing factor 3a subunit 1 (71 kDa, Protein from Homo sapiens)
116 kda u5 small nuclear ribonucleoprotein component (95 kDa, Protein from Homo sapiens)
U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
Probable atp-dependent rna helicase ddx23 (Complex from Homo sapiens)
U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
U6 snrna (34 kDa, RNA from Homo sapiens)
Guanosine-5'-triphosphate (523 Da, Ligand)
Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
Serine/threonine-protein kinase prp4 homolog (117 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
U2 snrna (60 kDa, RNA from Homo sapiens)
Phd finger-like domain-containing protein 5a (11 kDa, Protein from Homo sapiens)
U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
Splicing factor 3a subunit 2 (24 kDa, Protein from Homo sapiens)
U5 snrna (37 kDa, RNA from Homo sapiens)
Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
U1 small nuclear ribonucleoprotein a (31 kDa, Protein from Homo sapiens)
U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
Adml pre-mrna (15 kDa, RNA from Human adenovirus 2)
U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
Adml pre-mrna (Complex from Human adenovirus 2)
Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
Nhp2-like protein 1 (14 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
Fully-assembled precatalytic spliceosome (pre-b complex). (Complex from Homo sapiens)
U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Homo sapiens)
U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
Pre-mrna-processing factor 6 (97 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Inositol hexakisphosphate (660 Da, Ligand)
U4 snrna (46 kDa, RNA from Homo sapiens)
Zinc ion (65 Da, Ligand)
Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
U1 snrna (52 kDa, RNA from Homo sapiens)
U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
Fully-assembled precatalytic spliceosome (pre-b complex). (3 MDa, Complex)
U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)

[Show more]

EMD-6972

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Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part)

Release date: Aug. 29, 2018
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5zwm
Q-score: 0.162

Bai R, Wan R, Yan C, Lei J, Shi Y

Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]

Sample:

U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-bps (13 kDa, RNA from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast fully assembled spliceosomal pre-b complex before activation (Cell from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)

[Show more]

EMD-6974

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Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom

Release date: Aug. 29, 2018
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5zwo
Q-score: 0.191

Bai R, Wan R, Yan C, Lei J, Shi Y

Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]

Sample:

U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor spp381 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
23 kda u4/u6.u5 small nuclear ribonucleoprotein component (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast fully assembled spliceosomal b complex before activation (Cell from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna (19 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 38 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)

[Show more]

EMD-3683

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Structure of a pre-catalytic spliceosome (B2 map)

Release date: May 31, 2017
Resolution: 7.2 Å
EM Method: Single-particle
Fitted PDBs: 5nrl
Q-score: 0.117

Plaschka C, Lin PC, Nagai K

Nature (2017) 546 pp. 617-621 [ Pubmed: 28530653 DOI: doi:10.1038/nature22799 ]

Sample:

Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor spp381 (33 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Unknown (7 kDa, Protein from Saccharomyces cerevisiae)
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae )
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
Yeast ubc4 gene for ubiquitin-conjugating enzyme (30 kDa, RNA from Saccharomyces cerevisiae)
23 kda u4/u6.u5 small nuclear ribonucleoprotein component (22 kDa, Protein from Saccharomyces cerevisiae)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae)
Guanosine-5'-triphosphate (523 Da, Ligand)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae )
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae)
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 38 (27 kDa, Protein from Saccharomyces cerevisiae)
Pre-catalytic b complex spliceosome (2 MDa, Complex from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae )
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae)

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EMD-8012

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The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom

Release date: Jan. 27, 2016
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 5gan
Q-score: 0.254

Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K

Nature (2016) 530 pp. 298-302 [ DOI: doi:10.1038/nature16940 Pubmed: 26829225 ]

Sample:

Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae)
Guanosine-5'-triphosphate (523 Da, Ligand)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae)
Unknown protein (8 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae)
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae)
Snu66 (28 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae)
The overall structure of the yeast spliceosomal u4/u6.u5 tri-snrnp (1 MDa, Complex from Saccharomyces cerevisiae)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae)
U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae)

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