Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6qee
Q-score: 0.425
Alam A, Kowal J, Broude E, Roninson I, Locher KP
Science (2019) 363 pp. 753-756 [ DOI: doi:10.1126/science.aav7102 Pubmed: 30765569 ]
- Zosuquidar (527 Da, Ligand)
- Abcb1hm-eq (143 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Uic2 fab (Complex from Mus musculus)
- Uic2 antigen binding fragment light chain (24 kDa, Protein from Mus musculus)
- Nanodisc reconstituted abcb1hm (human mouse chimeric abcb1) eq mutant in complex with uic2 fab and zosuquidar (200 kDa, Complex)
- Cholesterol (386 Da, Ligand)
- Uic2 antigen binding fragment heavy chain (24 kDa, Protein from Mus musculus)
- Abcb1hm (Complex from Homo sapiens)
Zosuquidar and UIC2 Fab complex of human-mouse chimeric ABCB1 (ABCB1HM)
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 6fn1
Q-score: 0.442
Alam A, Kung R, Kowal J, McLeod RA, Tremp N, Broude EV, Roninson IB, Stahlberg H, Locher KP
PNAS (2018) 115 pp. E1973-E1982 [ DOI: doi:10.1073/pnas.1717044115 Pubmed: 29440498 ]
- Zosuquidar (527 Da, Ligand)
- Human-mouse chimeric abcb1 (abcbhm) (137 kDa, Protein from Homo sapiens)
- Human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab and zosuquidar (195 kDa, Complex)
- Human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab and zosuquidar (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Uic2 antigen binding fragment light chain (24 kDa, Protein from Mus musculus)
- Human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab and zosuquidar (Complex from Mus musculus)
- Uic2 antigen binding fragment heavy chain (24 kDa, Protein from Mus musculus)
Apo form of UIC2 Fab complex of human-mouse chimeric ABCB1 (ABCB1HM)
Resolution: 4.14 Å
EM Method: Single-particle
Fitted PDBs: 6fn4
Q-score: 0.34
Alam A, Kung R, Kowal J, McLeod RA, Tremp N, Broude EV, Roninson IB, Stahlberg H, Locher KP
PNAS (2018) 115 pp. E1973-E1982 [ DOI: doi:10.1073/pnas.1717044115 Pubmed: 29440498 ]
- Apo form of human-mouse chimeric abcb1 (abcb1hm) in complex with antigen binding fragment of uic2. (141 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Apo human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab (195 kDa, Complex)
- Uic2 antigen binding fragment light chain (24 kDa, Protein from Mus musculus)
- Apo human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab (142 kDa, Complex from Homo sapiens)
- Apo human-mouse chimeric abcb1 (abcb1hm) complex with uic2 fab (48 kDa, Complex from Mus musculus)
- Uic2 antigen binding fragment heavy chain (24 kDa, Protein from Mus musculus)