Structure of urate bound human SLC2A9 transporter
Khandelwal NK, Stroud RM, Gupta MG
To Be Published
- Soluble cytochrome b562,solute carrier family 2, facilitated glucose transporter member 9 (69 kDa, Protein from Homo sapiens)
- Uric acid (168 Da, Ligand)
- Codon optimize slc2a9 gene was synthesize and cloned in 83 nu yeast expressing vector from genscript. protein expressed in yeast cells and purified using ni-affinity followed by sizing. (69 kDa, Complex from Homo sapiens (Human))
Structure of human URAT1 bound with urate
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 9irw
Q-score: 0.519
To Be Published
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Uric acid (168 Da, Ligand)
- Human urate transporter 1 (Complex from Homo sapiens)
Human URAT1 bound with Uric acid
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 9jdv
Q-score: 0.344
Wu C, Zhang C, Jin S, Wang JJ, Dai A, Xu J, Zhang H, Yang X, He X, Yuan Q, Hu W, Xu Y, Wang M, Jiang Y, Yang D, Xu HE
bioRxiv (2024) [ DOI: doi:10.1101/2024.09.11.612394 ]
- Human uric acid transporter 1 with uric acid (Complex from Homo sapiens)
- Uric acid (168 Da, Ligand)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
Human URAT1 bound with verinurad
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9jdy
Q-score: 0.386
Wu C, Zhang C, Jin S, Wang JJ, Dai A, Xu J, Zhang H, Yang X, He X, Yuan Q, Hu W, Xu Y, Wang M, Jiang Y, Yang D, Xu HE
bioRxiv (2024) [ DOI: doi:10.1101/2024.09.11.612394 ]
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Human uric acid transportor 1 with verinurad (Complex from Homo sapiens)
- Verinurad (348 Da, Ligand)
Human URAT1 bound to lesinurad
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9jdz
Q-score: 0.361
Wu C, Zhang C, Jin S, Wang JJ, Dai A, Xu J, Zhang H, Yang X, He X, Yuan Q, Hu W, Xu Y, Wang M, Jiang Y, Yang D, Xu HE
bioRxiv (2024) [ DOI: doi:10.1101/2024.09.11.612394 ]
- Human uric acid transportor 1 with lesinurad (Complex from Homo sapiens)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Lesinurad (404 Da, Ligand)
Human URAT1 bound to benzbromarone
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9je0
Q-score: 0.363
Wu C, Zhang C, Jin S, Wang JJ, Dai A, Xu J, Zhang H, Yang X, He X, Yuan Q, Hu W, Xu Y, Wang M, Jiang Y, Yang D, Xu HE
bioRxiv (2024) [ DOI: doi:10.1101/2024.09.11.612394 ]
- Water (18 Da, Ligand)
- [3,5-bis(bromanyl)-4-oxidanyl-phenyl]-(2-ethyl-1-benzofuran-3-yl)methanone (424 Da, Ligand)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Human uric acid transporter 1 with benzbromarone (Complex from Homo sapiens)
Human URAT1 bound to dotinurad
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9je1
Q-score: 0.359
Wu C, Zhang C, Jin S, Wang JJ, Dai A, Xu J, Zhang H, Yang X, He X, Yuan Q, Hu W, Xu Y, Wang M, Jiang Y, Yang D, Xu HE
bioRxiv (2024) [ DOI: doi:10.1101/2024.09.11.612394 ]
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Dotinurad (358 Da, Ligand)
- Human uric acid transporter 1 with dotinurad (Complex from Homo sapiens)
Urate bound human URAT1 in the inward-facing state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9b1j
Q-score: 0.494
Cell Res (2024) 34 pp. 776-787 [ Pubmed: 39245778 DOI: doi:10.1038/s41422-024-01023-1 ]
- Phosphate ion (94 Da, Ligand)
- Uric acid (168 Da, Ligand)
- Urate bound human urat1 in the inward-facing state (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
Urate bound human URAT1 in the occluded state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9b1k
Q-score: 0.448
Cell Res (2024) 34 pp. 776-787 [ Pubmed: 39245778 DOI: doi:10.1038/s41422-024-01023-1 ]
- Uric acid (168 Da, Ligand)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)
- Urate bound human urat1 in the occluded state. (Complex from Homo sapiens)
Urate bound human URAT1 in the outward-facing state.
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9b1l
Q-score: 0.512
Cell Res (2024) 34 pp. 776-787 [ Pubmed: 39245778 DOI: doi:10.1038/s41422-024-01023-1 ]
- Uric acid (168 Da, Ligand)
- Urate bound human urat1 in the outward-facing state. (Complex from Homo sapiens)
- Solute carrier family 22 member 12 (59 kDa, Protein from Homo sapiens)