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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Unassigned" AND current_status:"REL" AND author_name:"Sm"

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EMD-12106

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Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map

Release date: March 10, 2021
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b9v
Q-score: 0.378

Wilkinson ME, Fica SM, Galej WP, Nagai K

Mol Cell (2021) 81 pp. 1439- [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]

Sample:

Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 18 (28 kDa, Protein from Saccharomyces cerevisiae)
5' exon of ubc4 mrna (15 kDa, RNA from Saccharomyces cerevisiae)
Yeast c complex spliceosome with prp18/slu7 bound (2 MDa, Complex from Saccharomyces cerevisiae)
Syf1 isoform 1 (100 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae)
Unassigned structure (20 kDa, Protein from Saccharomyces cerevisiae)
Ntc20 isoform 1 (15 kDa, Protein from Saccharomyces cerevisiae)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
Cwc22 isoform 1 (67 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
Potassium ion (39 Da, Ligand)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Saccharomyces cerevisiae)
Branched intron and 3' exon of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
Bj4_g0054360.mrna.1.cds.1 (50 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
Cdc40 isoform 1 (52 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae)
D-chiro inositol hexakisphosphate (660 Da, Ligand)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor isy1 (27 kDa, Protein from Saccharomyces cerevisiae)
Clf1 isoform 1 (82 kDa, Protein from Saccharomyces cerevisiae)
Bud31 isoform 1 (18 kDa, Protein from Saccharomyces cerevisiae)
Y55_g0042700.mrna.1.cds.1 (67 kDa, Protein from Saccharomyces cerevisiae)
Magnesium ion (24 Da, Ligand)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Saccharomyces cerevisiae)
Snt309 isoform 1 (20 kDa, Protein from Saccharomyces cerevisiae)
Splicing factor yju2 (33 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae)

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EMD-0523

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Yeast Ctf19 complex

Release date: April 3, 2019
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 6nuw
Q-score: 0.342

Hinshaw SM, Harrison SC

eLife (2019) 8 [ Pubmed: 30762520 DOI: doi:10.7554/eLife.44239 ]

Sample:

Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit mcm22 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Ctf19 complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit chl4 (53 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Unknown (unassigned) (1 kDa, Protein from Saccharomyces cerevisiae S288c)
Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit ctf3,inner kinetochore subunit ctf3 (87 kDa, Protein from Saccharomyces cerevisiae S288c)
Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Inner kinetochore subunit mcm16 (6 kDa, Protein from Saccharomyces cerevisiae S288c)
Inner kinetochore subunit ame1 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-3979

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Post-catalytic P complex spliceosome with 3' splice site docked

Release date: Jan. 17, 2018
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6exn
Q-score: 0.296

Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K

Science (2017) 358 pp. 1283-1288 [ DOI: doi:10.1126/science.aar3729 Pubmed: 29146871 ]

Sample:

Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
U6 snrna (35 kDa, RNA from Baker's yeast)
Pre-mrna-splicing factor prp8 (279 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
Unassigned structure (8 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor atp-dependent rna helicase prp22 (130 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
U5 snrna (57 kDa, RNA from Baker's yeast)
3' exon (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor prp18 (28 kDa, Protein from Baker's yeast)
Ligated exons: ubc4 mrna (12 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
Intron lariat: ubc4 rna (30 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
Magnesium ion (24 Da, Ligand)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp19 (56 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
Inositol hexakisphosphate (660 Da, Ligand)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
Pre-mrna-processing factor prp17 (52 kDa, Protein from Baker's yeast)
P complex spliceosome with 3' exon docked (2 MDa, Complex)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
P complex spliceosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
Pre-mrna-splicing factor cwc16 (32 kDa, Protein from Baker's yeast)

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Sort by: Download

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EMDB [3]

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